Mitochondria Antibody (AE-1) - IHC-Prediluted

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Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282] - Human Tonsil stained with Mitochondria Monoclonal Antibody (AE-1).
Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282] - Human Tonsil stained with Mitochondria Mouse Monoclonal Antibody (AE-1).
Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282] - Human Pancreas stained with Mitochondria Mouse Monoclonal Antibody (AE-1).
Immunohistochemistry-Paraffin: Mitochondria Antibody (AE-1) - IHC-Prediluted [NBP2-48282] - Human Pancreas stained with Mitochondria Monoclonal Antibody (AE-1).

Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications IHC, IHC-P
Clone
AE-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

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Mitochondria Antibody (AE-1) - IHC-Prediluted Summary

Immunogen
Semi-Purified mitochondrial preparation
Localization
Mitochondria in cytoplasm
Marker
Marker for Human Cells
Specificity
This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes) The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Mouse or Rat.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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