Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7550APC | |||
| Biotin | NB7550B | |||
| DyLight 350 | NB7550UV | |||
| DyLight 405 | NB7550V | |||
| DyLight 488 | NB7550G | |||
| DyLight 550 | NB7550R | |||
| DyLight 594 | NB7550DL594 | |||
| DyLight 650 | NB7550C | |||
| DyLight 680 | NB7550FR | |||
| DyLight 755 | NB7550IR | |||
| FITC | NB7551 | |||
| HRP | NB7552 | |||
| Janelia Fluor 525 | NB7550JF525 | |||
| Janelia Fluor 669 | NB7550JF669 | |||
| PE | NB7550PE | |||
| PerCP | NB7550PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54488-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51590SS | |||
| Azide and BSA Free | NBP2-80874 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26564-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26333-50ul | |||
![N/A Atrial Natriuretic Peptide/ANP [Biotin]](http://images.novusbio.com/fullsize2/DATA_Atrial_Natriuretic_Peptide_DY824705_ELISA_2538.jpg "N/A Atrial Natriuretic Peptide/ANP [Biotin]")
![Western Blot: Goat anti-Mouse Lambda Light Chain Secondary Antibody [NB7550] - Western Blot: Goat anti-Mouse Lambda Light Chain Secondary Antibody [HRP] [NB7552] - Goat anti-Mouse lambda Light Chain Antibody [HRP] [NB7552] - This secondary antibody was used for detection of beta-Actin in human pancreatic cancer cell lysates using beta-Actin antibody (Cat# NBP1-47423). Image from verified customer review. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Mouse-Lambda-Light-Chain-Secondary-Antibody-Western-Blot-NB7550-img0001.jpg "Western Blot: Goat anti-Mouse Lambda Light Chain Secondary Antibody [NB7550] - Western Blot: Goat anti-Mouse Lambda Light Chain Secondary Antibody [HRP] [NB7552] - Goat anti-Mouse lambda Light Chain Antibody [HRP] [NB7552] - This secondary antibody was used for detection of beta-Actin in human pancreatic cancer cell lysates using beta-Actin antibody (Cat# NBP1-47423). Image from verified customer review. Image using the HRP form of this antibody.")
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Western Blot Analysis of human HeLa cell lysate using Spectrin beta 3 Antibody (SPTBN2/1582).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Western-Blot-NBP2-53236-img0007.jpg "Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Western Blot Analysis of human HeLa cell lysate using Spectrin beta 3 Antibody (SPTBN2/1582).")
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta 3 Antibody (SPTBN2/1582).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Immunohistochemistry-Paraffin-NBP2-53236-img0005.jpg)
![Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Flow Cytometric Analysis of HeLa cells using Spectrin beta 3 Antibody (SPTBN2/1582). Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Flow-Cytometry-NBP2-53236-img0004.jpg)
![Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Analysis of HeLa and 293 Cell Lysates using Spectrin beta III Monoclonal Antibody (SPTBN2/1582).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Western-Blot-NBP2-53236-img0001.jpg)
![Protein Array: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta 3 Antibody (SPTBN2/1582). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-SPTBN2-1582-Protein-Array-NBP2-53236-img0006.jpg)
![N/A ICOS [Biotin]](http://images.novusbio.com/fullsize2/DATA_ICOS_DY169_ELISA_1698.jpg "N/A ICOS [Biotin]")
![Western Blot: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis using p75NTR/NGF Receptor mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/NGF-R-TNFRSF16-p75NTR-Antibody-2F1C2-Western-Blot-NBP1-51590-img0009.jpg "Western Blot: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis using p75NTR/NGF Receptor mouse mAb against NGFR-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis of EC cells using p75NTR/NGF Receptor mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/NGF-R-TNFRSF16-p75NTR-Antibody-2F1C2-Immunocytochemistry-Immunofluorescence-NBP1-51590-img0008.jpg)
![Flow Cytometry: NGF R/TNFRSF16/p75NTR Antibody (2F1C2) [NBP1-51590] - Analysis of EC cells using p75NTR/NGF Receptor mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NGF-R-TNFRSF16-p75NTR-Antibody-2F1C2-Flow-Cytometry-NBP1-51590-img0007.jpg)
![Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Western blot using affinity purified anti-Fibroblast Activation protein alpha/Fibroblast Activation Protein alpha/FAP antibody shows detection of Fibroblast Activation Protein alpha/FAP protein in whole cell lysates from Fibroblast Activation Protein alpha/FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected Fibroblast Activation Protein alpha/FAP. Primary antibody was used at 1:1,000.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-Western-Blot-NB110-85534-img0006.jpg "Western Blot: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Western blot using affinity purified anti-Fibroblast Activation protein alpha/Fibroblast Activation Protein alpha/FAP antibody shows detection of Fibroblast Activation Protein alpha/FAP protein in whole cell lysates from Fibroblast Activation Protein alpha/FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3). Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively). The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected Fibroblast Activation Protein alpha/FAP. Primary antibody was used at 1:1,000.")
![ELISA: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - ELISA results of purified Fibroblast Activation protein alpha/FAP antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/Fibroblast-Activation-Protein-alpha-FAP-Antibody-ELISA-NB110-85534-img0007.jpg)
![Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [NB110-85534] - Specific co-culture system created to study human macrophage cancer cells' conduction of MMT process, and Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488] (Catalog # NB110-85534AF488) was used as a myo-CAFs marker. Image from a verified customer review.](http://images.novusbio.com/fullsize/nb110-85534_rabbit-polyclonal-fibroblast-activation-protein-alpha-fap-antibody-71020241239278.png)
![Immunoprecipitation: FUBP1 Antibody (7C3) [NBP3-26564] - Immunoprecipitating FUBP1 in Jurkat whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26564 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26564 (2ug) + Jurkat whole cell lysate (500ug). Lane 3: Jurkat whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-2622024132376.jpg "Immunoprecipitation: FUBP1 Antibody (7C3) [NBP3-26564] - Immunoprecipitating FUBP1 in Jurkat whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26564 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26564 (2ug) + Jurkat whole cell lysate (500ug). Lane 3: Jurkat whole cell lysate (10ug).")
![Immunohistochemistry: FUBP1 Antibody (7C3) [NBP3-26564] - Image of FUBP1 Antibody (7C3) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-26220241535570.jpg)
![Immunohistochemistry: FUBP1 Antibody (7C3) [NBP3-26564] - Image of FUBP1 Antibody (7C3) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-262202415355746.jpg)
![Western Blot: FUBP1 Antibody (7C3) [NBP3-26564] - Positive Western Blot detected in: Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate. All lanes: FUBP1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 68, 69 kDa Observed band size: 69 kDa](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-282202411442642.jpg)
![Immunocytochemistry/Immunofluorescence: FUBP1 Antibody (7C3) [NBP3-26564] - Staining of Hela Cells with FUBP1 Antibody (7C3) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-282202412475147.jpg)
![Flow Cytometry: FUBP1 Antibody (7C3) [NBP3-26564] - Overlay histogram showing Jurkat cells stained with FUBP1 Antibody (7C3) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26564_rabbit-fubp1-mab-7c3-282202414312710.jpg)
![N/A TIM-3 [HRP]](http://images.novusbio.com/fullsize2/TIM3_DTIM30_ELISA_1115.jpg "N/A TIM-3 [HRP]")
![N/A TIM-3 [HRP]](http://images.novusbio.com/fullsize2/TIM3_DTIM30_ELISA_1114.jpg)
![Western Blot: E-Tag Epitope Tag Antibody [NB600-518] - Detection of E-Tag-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-E-Tag antibody NB600-518 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/E-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-518-img0004.jpg "Western Blot: E-Tag Epitope Tag Antibody [NB600-518] - Detection of E-Tag-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-E-Tag antibody NB600-518 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0003.jpg "Western Blot: Nrf1 Antibody [NBP1-77822] - Lane: NFR1-HIS recombinant protein. Load: 50ug per lane. Primary antibody - 1: NRF1 antibody at 1:1,000 overnight at 4C. Primary antibody - 2: 6xHIS Epitope tag antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/observed size: 67 kDa, 67 kDa for NRF1-His tagged. Other band(s): None.")
![Immunohistochemistry: Nrf1 Antibody [NBP1-77822] - Used at a 5 ug/ml to detect NRF1 in a variety of tissues. This image shows NRF1 staining of human testis. The antibody shows nuclear staining in lymphocytes. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/Nrf1-Antibody-Immunohistochemistry-NBP1-77822-img0004.jpg)
![Western Blot: Nrf1 Antibody [NBP1-77822] - Shows detection of a 67-kDa band corresponding to human NRF1 in a (lane 1) HeLa nuclear extract and (lane 2) whole cell lysate (molecular weight marker not shown). Approx. 10 ug of each lysate was separated by SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye (TM) 700 labeled Goat-a-Rabbit IgG [H&L] diluted 1:2,500. IRDye (TM) 700](http://images.novusbio.com/fullsize/Nrf1-Antibody-Western-Blot-NBP1-77822-img0002.jpg)
![Western Blot: Syntenin 1 Antibody (19C1) [NBP3-26333] - Positive Western Blot detected in: HT29 whole cell lysate, U87 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate. All lanes: Syntenin 1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa Observed band size: 32 kDa](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-28220241147217.jpg)
![Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody (19C1) [NBP3-26333] - Staining of Hela with Syntenin 1 Antibody (19C1) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 521-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-28220241249364.jpg)
![Flow Cytometry: Syntenin 1 Antibody (19C1) [NBP3-26333] - Overlay Peak curve showing HepG2 cells stained with Syntenin 1 Antibody (19C1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-282202414312716.jpg)