Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-18651-0.02mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB44782U-100UG | |||
| Alexa Fluor 405 | FAB44782V-100UG | |||
| Alexa Fluor 488 | FAB44782G-100UG | |||
| Alexa Fluor 594 | FAB44782T-100UG | |||
| Alexa Fluor 647 | FAB44782R-100UG | |||
| Alexa Fluor 700 | FAB44782N-100UG | |||
| Alexa Fluor 750 | FAB44782S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB44782-SP | |||
| Carrier Free | FAB44782V-100UG | |||
| Carrier Free | FAB44782S-100UG | |||
| Carrier Free | FAB44782N-100UG | |||
| Carrier Free | FAB44782R-100UG | |||
| Carrier Free | FAB44782U-100UG | |||
| Carrier Free | FAB44782G-100UG | |||
| Carrier Free | FAB44782T-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-53103-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB32653-SP | |||
![Immunohistochemistry: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Saphenous vein wall harvested from a patient diagnosed with triple-vessel CAD. Histological sections of a graft wall obtained from a 71-year-old patient who developed early graft failure 11 months after coronary artery bypass grafting. VEGFR-3 (b) is shown to be expressed by immunohistochemical staining in smooth muscle cells of the tunica media (TM) and is strongly present in endothelial cells of the tunica intima (TI). Image collected and cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VEGFR3-Flt-4-Antibody-9D9-Immunohistochemistry-NBP1-18651-img0011.jpg "Immunohistochemistry: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Saphenous vein wall harvested from a patient diagnosed with triple-vessel CAD. Histological sections of a graft wall obtained from a 71-year-old patient who developed early graft failure 11 months after coronary artery bypass grafting. VEGFR-3 (b) is shown to be expressed by immunohistochemical staining in smooth muscle cells of the tunica media (TM) and is strongly present in endothelial cells of the tunica intima (TI). Image collected and cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9) licensed under a CC-BY license.")
![Western Blot: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Analysis using recombinant human soluble VEGF Receptor 3 as target.](http://images.novusbio.com/fullsize/VEGFR3-Flt-4-Antibody-9D9-Western-Blot-NBP1-18651-img0010.jpg)
![Flow Cytometry: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Fig. 1: Flow analysis of VEGFR-3/FLT4 expression in primary human dermal lymphatic endothelial cells (HDLEC).](http://images.novusbio.com/fullsize/VEGFR3-Flt-4-Antibody-9D9-Flow-Cytometry-NBP1-18651-img0007.jpg)
![Flow Cytometry: VEGFR3/Flt-4 Antibody (9D9) [NBP1-18651] - Fig. 1: Flow analysis of VEGFR-3/FLT4 expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells (HUVEC). There is only a very weak expression of VEGFR-3/FLT4 detectable in the HUVECs but a strong one in the HDMVEC.](http://images.novusbio.com/fullsize/VEGFR3-Flt-4-Antibody-9D9-Flow-Cytometry-NBP1-18651-img0006.jpg)
![Immunohistochemistry: VEGFR3/Flt-4 Antibody (9D9) - Azide and BSA Free [NBP1-18651] - Saphenous vein wall harvested from a patient diagnosed with double-vessel CAD showing immunohistochemical staining. VEGF-C (a) & VEGFR-3 (b) reactivity in a graft wall obtained from a 67-year-old patient who developed the early graft restenosis within 7 months after coronary artery bypass grafting (the same patient as mentioned in Fig. 6). VEGF-C is localized exclusively within individual smooth muscle cells of the tunica media (TM). Cells with positive VEGFR-3 expression are situated in endothelial cells of the tunica intima (TI). c Negative control. TA tunica adventitia Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-18651_mouse-monoclonal-vegfr3-flt-4-antibody-9d9-310202415363714.jpg)
![Immunohistochemistry: VEGFR3/Flt-4 Antibody (9D9) - Azide and BSA Free [NBP1-18651] - Immunohistochemical staining of an internal thoracic artery & saphenous vein wall harvested from a patient with no evidence of luminal stenosis 12 months after CABG. Immunohistochemical staining of VEGF-C (a) & CAV2 (b) in an internal thoracic artery (ITA) graft & VEGFR-3 (c) in saphenous vein (SV) transplant harvested from a 65-year-old patient diagnosed with triple-vessel CAD. Note that lack of CAV2 expression within smooth muscle cells (SMC) in ITA wall is accompanied by VEGF-C positive expression in the same area. In SV tissue sample harvested from the same patient, no VEGRR-3 expression was found. d Negative control. TI tunica intima, TM tunica media, TA tunica adventitia Image collected & cropped by CiteAb from the following publication (//link.springer.com/10.1007/s00380-018-1158-9), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-18651_mouse-monoclonal-vegfr3-flt-4-antibody-9d9-310202415394521.jpg)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody directed against H3K36me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Western-Blot-NBP2-59199-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - Histone extracts (15 ug) from HeLa cells were analysed by Western blot using the antibody directed against H3K36me3 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~17 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - ChIP assays were performed using HeLa cells, the antibody against H3K36me3 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. QPCR was performed with primers for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the Sat2 satellite repeat. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-59199-img0003.jpg)
![ELISA: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K36me3 and the crude serum. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:19,300.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-ELISA-NBP2-59199-img0002.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-59199] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K36me3 with peptides containing other H3 and H4 modifications and the unmodified sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Dot-Blot-NBP2-59199-img0005.jpg)
![Western Blot: DNA polymerase theta Antibody (1C11) [H00010721-M09]](http://images.novusbio.com/fullsize/DNA-polymerase-theta-Antibody-1C11-Western-Blot-H00010721-M09-img0002.jpg "Western Blot: DNA polymerase theta Antibody (1C11) [H00010721-M09]")
![ELISA: DNA polymerase theta Antibody (1C11) [H00010721-M09] - Detection limit for recombinant GST tagged POLQ is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DNA-polymerase-theta-Antibody-1C11-ELISA-H00010721-M09-img0001.jpg)
![Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0006.jpg "Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat thymus tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.")
![Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Formalin fixed human thyroid cancer slices were were stained with a NBP2-75896 at 5 ug/ml.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0003.jpg)
![Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat epididymis tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0004.jpg)
![Immunohistochemistry: CD52 Antibody (Campath-1H) [NBP2-75896] - Anti-CD52 staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of Campath-1H (NBP2-75896) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Immunohistochemistry-NBP2-75896-img0005.jpg)
![Immunofluorescence: CD52 Antibody (Campath-1H) [NBP2-75896] - Formaldehyde-fixed rat thymus slices were stained with NBP2-75896 at 5 ug/ml and detected with a FITC-conjugated secondary antibody.](http://images.novusbio.com/fullsize/CD52-Antibody-Campath-1H-Immunofluorescence-NBP2-75896-img0002.jpg)
![Western Blot: Galectin-1 Antibody (GAL1/1831) - Azide and BSA Free [NBP2-54457] - Western Blot Analysis of JEG-3 and K562 cell lysate using Galectin-1 antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Azide-and-BSA-Free-Western-Blot-NBP2-54457-img0006.jpg "Western Blot: Galectin-1 Antibody (GAL1/1831) - Azide and BSA Free [NBP2-54457] - Western Blot Analysis of JEG-3 and K562 cell lysate using Galectin-1 antibody (GAL1/1831).")
![Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody (GAL1/1831) - Azide and BSA Free [NBP2-54457] - Confocal immunofluorescence image of HeLa cells using Galectin-1 Antibody (GAL1/1831). Green (CF488) and Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-54457-img0004.jpg)
![Immunohistochemistry-Paraffin: Galectin-1 Antibody (GAL1/1831) - Azide and BSA Free [NBP2-54457] - Formalin-fixed, paraffin-embedded Human Prostate Carcinoma stained with Galectin-1 Monoclonal Antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54457-img0001.jpg)
![Western Blot: Galectin-1 Antibody (GAL1/1831) - Azide and BSA Free [NBP2-54457] - HeLa, A431, K562 and 293 Cell Lysates using Galectin-1 Monoclonal Antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Azide-and-BSA-Free-Western-Blot-NBP2-54457-img0002.jpg)
![Western Blot: Galectin-1 Antibody (GAL1/1831) - Azide and BSA Free [NBP2-54457] - Western Blot Analysis of human HeLa cell lysate using Galectin-1 Antibody (GAL1/1831).](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Azide-and-BSA-Free-Western-Blot-NBP2-54457-img0005.jpg)
![Protein Array: Galectin-1 Antibody (GAL1/1831) - Azide and BSA Free [NBP2-54457] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Galectin-1 Antibody (GAL1/1831). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-GAL1-1831-Azide-and-BSA-Free-Protein-Array-NBP2-54457-img0003.jpg)
![Western Blot: Ferritin Light Chain Antibody (FTL/1386) - Azide and BSA Free [NBP2-54574] - Western Blot Analysis of human HeLa cell lysate using Ferritin Light Chain Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Azide-and-BSA-Free-Western-Blot-NBP2-54574-img0005.jpg "Western Blot: Ferritin Light Chain Antibody (FTL/1386) - Azide and BSA Free [NBP2-54574] - Western Blot Analysis of human HeLa cell lysate using Ferritin Light Chain Antibody (FTL/1386).")
![Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) - Azide and BSA Free [NBP2-54574] - Formalin-fixed, paraffin-embedded Human Testicular Carcinoma stained with Ferritin Light Chain Mouse Monoclonal Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54574-img0009.jpg)
![Western Blot: Ferritin Light Chain Antibody (FTL/1386) - Azide and BSA Free [NBP2-54574] - A431, HeLa, Liver and Testis Lysate using Ferritin, Light Chain Monoclonal Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Azide-and-BSA-Free-Western-Blot-NBP2-54574-img0003.jpg)
![Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) - Azide and BSA Free [NBP2-54574] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin, Light Chain Monoclonal Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54574-img0001.jpg)
![Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) - Azide and BSA Free [NBP2-54574] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin, Light Chain Monoclonal Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54574-img0002.jpg)
![Immunohistochemistry-Paraffin: Ferritin Light Chain Antibody (FTL/1386) - Azide and BSA Free [NBP2-54574] - Formalin-fixed, paraffin-embedded Human Pancreas stained with Ferritin Light Chain Mouse Monoclonal Antibody (FTL/1386).](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54574-img0008.jpg)
![Protein Array: Ferritin Light Chain Antibody (FTL/1386) - Azide and BSA Free [NBP2-54574] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ferritin Light Chain Antibody (FTL/1386) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Ferritin-Light-Chain-Antibody-FTL-1386-Azide-and-BSA-Free-Protein-Array-NBP2-54574-img0004.jpg)
![N/A CCL15/MIP-1 delta [Biotin]](http://images.novusbio.com/fullsize2/DATA_CCL15_DY363_ELISA_1966.jpg "N/A CCL15/MIP-1 delta [Biotin]")
![Immunohistochemistry-Paraffin: Progesterone Antibody (6-5E-10B) [NBP2-45210] - Mouse cervix tissue stained with Progesterone Antibody. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Progesterone-Antibody-6-5E-10B-Immunohistochemistry-Paraffin-NBP2-45210-img0002.jpg "Immunohistochemistry-Paraffin: Progesterone Antibody (6-5E-10B) [NBP2-45210] - Mouse cervix tissue stained with Progesterone Antibody. IHC-P image submitted by a verified customer review.")
![N/A: Progesterone Antibody (6-5E-10B) [NBP2-45210] - Molecular Structure of Progesterone](http://images.novusbio.com/fullsize/Progesterone-Antibody-6-5E-10B-NBP2-45210-img0001.jpg)
