Progesterone Antibody (6-5E-10B)

Immunohistochemistry-Paraffin: Progesterone Antibody (6-5E-10B) [NBP2-45210] - Mouse cervix tissue stained with Progesterone Antibody. IHC-P image submitted by a verified customer review.

N/A: Progesterone Antibody (6-5E-10B) [NBP2-45210] - Molecular Structure of Progesterone

• Reactivity: All-NA
• Applications: ELISA, Func, IHC
• Clone: 6-5E-10B
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Progesterone Antibody (6-5E-10B) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-53103)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Progesterone- 11a-hemisuccinate conjugated to bovine serum albumin (Pr11a-HMS-BSA)
• Localization: Secreted and cytoplasmic
• Specificity: This monoclonal antibody is specific for progesterone. It exhibits minimal cross reactivity with related compounds in ELISA. It reacts with Progesterone-11a-HMS-BSA: 100%; 5-beta-Pregnane-3,20-dione: 48%; 5-alpha-Pregnane-3,20-dione: 26.4%; 17-alpha-Hydroxyprogesterone: 2.5% and 20-alpha-Hydroxyprogesterone: 0.04%. Progesterone is a steroid hormone synthesized from the cholesterol derivative, pregnenolone, in the cortex of the adrenal gland. Progesterone is secreted by the corpus luteum and acts to prepare the endometrium for the implantation of a fertilized egg. During pregnancy, it is secreted by the placenta to prevent spontaneous abortion and to stimulate the development of mammary tissue to produce milk. Thus, progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Luteinized theca cells of normal ovary secrete progesterone. The determination of progesterone concentrations in the body fluids is of great value for endocrinological investigations in women. This monoclonal antibody may prove useful in identification of ovarian tumors.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Radioimmunoassay
• Application Notes: ELISA: For coating, order Ab without BSA.
  Immunohistochemistry (Not assessed). This Progesterone Antibody (6-5E-10B) is validated for IHC-P from a verified customer review.
• Theoretical MW: 314.46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Progesterone Antibody (6-5E-10B)

• 4-Pregnen-3,20-dione
• Progesterone

Background

Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain. Progesterone levels 1. men 30-60 pg/0.1ml 2. women pre ovulatory phase: 20-160 pg/0.1ml; ovulatory phase: 1,000-1,700 pg/0.1ml; post ovulatory phase: 1,000-1,700 pg/0.1ml; Pregnant: 16-18 weeks: 300-800 pg/0.1ml; 28-30 weeks: 6,500-14,700 pg/0.1ml; 38-40 weeks: 12,000-19,000 pg/0.1ml.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Progesterone Antibody (NBP2-45210)(1)

Publication using NBP2-45210

• Sewnarain S, Singh S, Naicker T Placental progesterone and its receptor in HIV-infected pre-eclamptic women Histochemistry and cell biology 2023-11-17 [PMID: 37975897] (IHC-P)

Reviews for Progesterone Antibody (NBP2-45210) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Vertebrate.

reviewed by: Verified Customer IHC-P Mouse 10/13/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse cervix
• Species: Mouse
• Lot: 6-5E-10B

Comments

• Comments: Primary antibody dilution 1:200, incubated at 4C for 10 hours, secondary antibody dilution 1:500

reviewed by: Verified Customer ELISA Vertebrate 12/15/2016

Summary

• Application: ELISA
• Sample Tested: Fecal
• Species: Vertebrate

Comments

• Comments: Elisa assay to test Cortisol in monkey samples