Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38508-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37891-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35145-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18336-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38305-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38071-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94059-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92315-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05019-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92719-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03009-20ul | |||
![Immunocytochemistry/ Immunofluorescence: Centrin 3 Antibody [NBP3-38508] - Immunofluorescence analysis of L929 cells using Centrin 3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38508_rabbit-centrin-3-pab-101220241159286.jpg "Immunocytochemistry/ Immunofluorescence: Centrin 3 Antibody [NBP3-38508] - Immunofluorescence analysis of L929 cells using Centrin 3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: Centrin 3 Antibody [NBP3-38508] - Immunofluorescence analysis of C6 cells using Centrin 3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38508_rabbit-centrin-3-pab-101220241224142.jpg)
![Immunocytochemistry/ Immunofluorescence: Centrin 3 Antibody [NBP3-38508] - Immunofluorescence analysis of U2OS cells using Centrin 3 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38508_rabbit-centrin-3-pab-1012202411592893.jpg)
![Immunohistochemistry: Centrin 3 Antibody [NBP3-38508] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Centrin 3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38508_rabbit-centrin-3-pab-101220241252852.jpg)
![Western Blot: Centrin 3 Antibody [NBP3-38508] - Western blot analysis of various lysates using Centrin 3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38508_rabbit-centrin-3-pab-1012202411592863.jpg)
![Immunohistochemistry: Centrin 3 Antibody [NBP3-38508] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Centrin 3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38508_rabbit-centrin-3-pab-10122024121079.jpg)
![Immunohistochemistry: Centrin 3 Antibody [NBP3-38508] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Centrin 3 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38508_rabbit-centrin-3-pab-101220241214498.jpg)
![Immunocytochemistry/ Immunofluorescence: WDR77 Antibody [NBP3-37891] - Immunofluorescence analysis of HeLa cells using WDR77 Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-37891_rabbit-wdr77-pab-91220241420134.jpg "Immunocytochemistry/ Immunofluorescence: WDR77 Antibody [NBP3-37891] - Immunofluorescence analysis of HeLa cells using WDR77 Rabbit pAb.Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution.")
![Immunoprecipitation: WDR77 Antibody [NBP3-37891] - Immunoprecipitation analysis of 200 ug extracts of 293T cells using 3 ug WDR77 antibody. Western blot was performed from the immunoprecipitate using WDR77 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-37891_rabbit-wdr77-pab-91220241581279.jpg)
![Immunohistochemistry: WDR77 Antibody [NBP3-37891] - Immunohistochemistry analysis of paraffin-embedded Human esophageal using WDR77 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37891_rabbit-wdr77-pab-912202414154345.jpg)
![Immunohistochemistry: WDR77 Antibody [NBP3-37891] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using WDR77 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37891_rabbit-wdr77-pab-912202414163038.jpg)
![Immunohistochemistry: WDR77 Antibody [NBP3-37891] - Immunohistochemistry analysis of paraffin-embedded Human colon using WDR77 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37891_rabbit-wdr77-pab-912202414112845.jpg)
![Immunohistochemistry: WDR77 Antibody [NBP3-37891] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using WDR77 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37891_rabbit-wdr77-pab-912202414123859.jpg)
![Western Blot: WDR77 Antibody [NBP3-37891] - Western blot analysis of lysates from 293T cells, using WDR77 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-37891_rabbit-wdr77-pab-91220241418195.jpg)
![Immunocytochemistry/ Immunofluorescence: AKR1C2 Antibody [NBP3-35145] - Immunofluorescence analysis of L929 cells using AKR1C2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35145_rabbit-akr1c2-pab-1012202411343318.jpg "Immunocytochemistry/ Immunofluorescence: AKR1C2 Antibody [NBP3-35145] - Immunofluorescence analysis of L929 cells using AKR1C2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: AKR1C2 Antibody [NBP3-35145] - Western blot analysis of various lysates, using AKR1C2 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35145_rabbit-akr1c2-pab-1012202411421018.jpg)
![Immunohistochemistry: AKR1C2 Antibody [NBP3-35145] - Immunohistochemistry analysis of paraffin-embedded Human Colon cancer using AKR1C2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35145_rabbit-akr1c2-pab-1012202411334036.jpg)
![Immunocytochemistry/ Immunofluorescence: AKR1C2 Antibody [NBP3-35145] - Immunofluorescence analysis of U-2 OS cells using AKR1C2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35145_rabbit-akr1c2-pab-1012202411334076.jpg)
![Immunohistochemistry: AKR1C2 Antibody [NBP3-35145] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using AKR1C2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35145_rabbit-akr1c2-pab-1012202411421024.jpg)
![Immunohistochemistry: AKR1C2 Antibody [NBP3-35145] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using AKR1C2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35145_rabbit-akr1c2-pab-1012202411381260.jpg)
![Immunohistochemistry: AKR1C2 Antibody [NBP3-35145] - Immunohistochemistry analysis of paraffin-embedded Rat liver using AKR1C2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35145_rabbit-akr1c2-pab-1012202411433424.jpg)
![Western Blot: IFI16 Antibody [NBP3-18336] - Western Blot Validation in Rat Testis. Loading: 15 ug of lysate per lane. Antibodies: IFI16 NBP3-18336, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/IFI16-Antibody-Western-Blot-NBP3-18336-img0003.jpg "Western Blot: IFI16 Antibody [NBP3-18336] - Western Blot Validation in Rat Testis. Loading: 15 ug of lysate per lane. Antibodies: IFI16 NBP3-18336, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.")
![Western Blot: IFI16 Antibody [NBP3-18336] - WB Validation in Human and Rat Cell Lines. Loading: 15 ug of lysate per lane. Antibodies: IFI16 NBP3-18336, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/IFI16-Antibody-Western-Blot-NBP3-18336-img0001.jpg)
![Western Blot: IFI16 Antibody [NBP3-18336] - Western Blot Validation in Mouse Tissues. Loading: 15 ug of lysates per lane. Antibodies: IFI16 NBP3-18336, 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/IFI16-Antibody-Western-Blot-NBP3-18336-img0002.jpg)
![Immunohistochemistry-Paraffin: IFI16 Antibody [NBP3-18336] - Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-IFI16 antibody (NBP3-18336) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/IFI16-Antibody-Immunohistochemistry-Paraffin-NBP3-18336-img0005.jpg)
![Immunohistochemistry-Paraffin: IFI16 Antibody [NBP3-18336] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IFI16 antibody (NBP3-18336) at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/IFI16-Antibody-Immunohistochemistry-Paraffin-NBP3-18336-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: IFI16 Antibody - BSA Free [NBP3-18336] - Validation of IFI16 in Human Molt4 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling IFI16 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-18336_rabbit-ifi16-pab-2711202419531689.jpg)
![Immunohistochemistry: IFI16 Antibody - BSA Free [NBP3-18336] - Validation of IFI16 in Rat Testis. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IFI16 antibody at 1 ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp3-18336_rabbit-ifi16-pab-271120242022951.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of NIH/3T3 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411545558.jpg "Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of NIH/3T3 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of A-549 cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-101220241233811.jpg)
![Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP3-38305] - Immunofluorescence analysis of U2OS cells using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411572633.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411531043.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-10122024120610.jpg)
![Immunohistochemistry: NFkB p105/p50 Antibody [NBP3-38305] - Immunohistochemistry analysis of paraffin-embedded Rat lung using NFkB p105/p50 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-101220241233849.jpg)
![Western Blot: NFkB p105/p50 Antibody [NBP3-38305] - Western blot analysis of lysates from Mouse lung, using NFkB p105/p50 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-38305_rabbit-nfkb-p105-p50-pab-1012202411572669.jpg)
![Western Blot: PSMA7 Antibody [NBP3-38071] - Western blot analysis of various lysates using PSMA7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38071_rabbit-psma7-pab-912202415121340.jpg "Western Blot: PSMA7 Antibody [NBP3-38071] - Western blot analysis of various lysates using PSMA7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunohistochemistry: PSMA7 Antibody [NBP3-38071] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using PSMA7 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38071_rabbit-psma7-pab-912202415175274.jpg)
![Immunocytochemistry/ Immunofluorescence: PSMA7 Antibody [NBP3-38071] - Immunofluorescence analysis of NIH-3T3 cells using PSMA7 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38071_rabbit-psma7-pab-912202414154324.jpg)
![Immunohistochemistry: PSMA7 Antibody [NBP3-38071] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PSMA7 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38071_rabbit-psma7-pab-912202414201345.jpg)
![Immunocytochemistry/ Immunofluorescence: PSMA7 Antibody [NBP3-38071] - Immunofluorescence analysis of U-2 OS cells using PSMA7 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38071_rabbit-psma7-pab-9122024159585.jpg)
![Immunohistochemistry: PSMA7 Antibody [NBP3-38071] - Immunohistochemistry analysis of paraffin-embedded Rat testis using PSMA7 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38071_rabbit-psma7-pab-912202414101812.jpg)
![Western Blot: PSMA7 Antibody [NBP3-38071] - Western blot analysis of lysates from rat liver, using PSMA7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38071_rabbit-psma7-pab-912202415111822.jpg)
![Western Blot: Nephronophthisis Antibody [NBP2-94059] - Analysis of extracts of Mouse pancreas, using NPHP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Nephronophthisis-Antibody-Western-Blot-NBP2-94059-img0003.jpg "Western Blot: Nephronophthisis Antibody [NBP2-94059] - Analysis of extracts of Mouse pancreas, using NPHP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Nephronophthisis Antibody [NBP2-94059] - Rat testis using NPHP1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Nephronophthisis-Antibody-Immunohistochemistry-Paraffin-NBP2-94059-img0005.jpg)
![Western Blot: Nephronophthisis Antibody [NBP2-94059] - Analysis of extracts of Mouse pancreas, using NPHP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Nephronophthisis-Antibody-Western-Blot-NBP2-94059-img0002.jpg)
![Immunohistochemistry-Paraffin: Nephronophthisis Antibody [NBP2-94059] - Rat kidney using NPHP1 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Nephronophthisis-Antibody-Immunohistochemistry-Paraffin-NBP2-94059-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Nephronophthisis Antibody - Azide and BSA Free [Nephronophthisis] - Immunofluorescence analysis of NIH/3T3 cells using Nephronophthisis Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94059_rabbit-polyclonal-nephronophthisis-antibody-52202516345245.jpg)
![Immunocytochemistry/ Immunofluorescence: Nephronophthisis Antibody - Azide and BSA Free [Nephronophthisis] - Immunofluorescence analysis of U2OS cells using Nephronophthisis Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94059_rabbit-polyclonal-nephronophthisis-antibody-5220251639651.jpg)
![Immunocytochemistry/ Immunofluorescence: Nephronophthisis Antibody - Azide and BSA Free [Nephronophthisis] - Immunofluorescence analysis of PC-12 cells using Nephronophthisis Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94059_rabbit-polyclonal-nephronophthisis-antibody-5220251638036.jpg)
![Western Blot: BOK Antibody [NBP2-92315] - Analysis of extracts of various cell lines, using BOK antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/BOK-Antibody-Western-Blot-NBP2-92315-img0001.jpg "Western Blot: BOK Antibody [NBP2-92315] - Analysis of extracts of various cell lines, using BOK antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: BOK Antibody [NBP2-92315] - Rat ovary using BOK Rabbit pAb (NBP2-92315) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BOK-Antibody-Immunohistochemistry-Paraffin-NBP2-92315-img0006.jpg)
![Immunohistochemistry-Paraffin: BOK Antibody [NBP2-92315] - Mouse spleen using BOK antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/BOK-Antibody-Immunohistochemistry-Paraffin-NBP2-92315-img0002.jpg)
![Immunohistochemistry-Paraffin: BOK Antibody [NBP2-92315] - Rat lung using BOK Rabbit pAb (NBP2-92315) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/BOK-Antibody-Immunohistochemistry-Paraffin-NBP2-92315-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: BOK Antibody - Azide and BSA Free [NBP2-92315] - Immunofluorescence analysis of MCF7 cells using BOK Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92315_rabbit-polyclonal-bok-antibody-42202514251868.jpg)
![Immunocytochemistry/ Immunofluorescence: BOK Antibody - Azide and BSA Free [NBP2-92315] - Immunofluorescence analysis of NIH/3T3 cells using BOK Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92315_rabbit-polyclonal-bok-antibody-4220251429014.jpg)
![Western Blot: BOK Antibody - Azide and BSA Free [NBP2-92315] - Western blot analysis of various lysates using BOK Rabbit pAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): U-937Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp2-92315_rabbit-polyclonal-bok-antibody-42202514275259.jpg)
![Western Blot: FRMD6 Antibody [NBP3-05019] - Analysis of extracts of various cell lines, using FRMD6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/FRMD6-Antibody-Western-Blot-NBP3-05019-img0006.jpg "Western Blot: FRMD6 Antibody [NBP3-05019] - Analysis of extracts of various cell lines, using FRMD6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: FRMD6 Antibody [NBP3-05019] - Analysis of U2OS cells using FRMD6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FRMD6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05019-img0002.jpg)
![Immunohistochemistry-Paraffin: FRMD6 Antibody [NBP3-05019] - Mouse heart using FRMD6 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FRMD6-Antibody-Immunohistochemistry-Paraffin-NBP3-05019-img0005.jpg)
![Western Blot: FRMD6 Antibody [NBP3-05019] - Analysis of extracts of various cell lines, using FRMD6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/FRMD6-Antibody-Western-Blot-NBP3-05019-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: FRMD6 Antibody [NBP3-05019] - Analysis of C6 cells using FRMD6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FRMD6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05019-img0001.jpg)
![Immunohistochemistry-Paraffin: FRMD6 Antibody [NBP3-05019] - Rat heart using FRMD6 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FRMD6-Antibody-Immunohistochemistry-Paraffin-NBP3-05019-img0003.jpg)
![Immunohistochemistry-Paraffin: FRMD6 Antibody [NBP3-05019] - Human thyroid cancer using FRMD6 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FRMD6-Antibody-Immunohistochemistry-Paraffin-NBP3-05019-img0004.jpg)
![Western Blot: DNA Ligase III Antibody [NBP2-92719] - Analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Western-Blot-NBP2-92719-img0005.jpg "Western Blot: DNA Ligase III Antibody [NBP2-92719] - Analysis of extracts of various cell lines, using at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: DNA Ligase III Antibody [NBP2-92719] - Analysis of PC-12 cells using LIG3 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92719-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: DNA Ligase III Antibody [NBP2-92719] - Analysis of NIH/3T3 cells using LIG3 Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/DNA-Ligase-III-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92719-img0010.jpg)
![Immunohistochemistry: DNA Ligase III Antibody - Azide and BSA Free [NBP2-92719] - Immunohistochemistry analysis of paraffin-embedded Rat liver using DNA Ligase III Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92719_rabbit-polyclonal-dna-ligase-iii-antibody-4220251429045.jpg)
![Immunohistochemistry: DNA Ligase III Antibody - Azide and BSA Free [NBP2-92719] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using DNA Ligase III Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92719_rabbit-polyclonal-dna-ligase-iii-antibody-4220251426446.jpg)
![Immunohistochemistry: DNA Ligase III Antibody - Azide and BSA Free [NBP2-92719] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DNA Ligase III Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92719_rabbit-polyclonal-dna-ligase-iii-antibody-42202514264477.jpg)
![Immunohistochemistry: DNA Ligase III Antibody - Azide and BSA Free [NBP2-92719] - Immunohistochemistry analysis of paraffin-embedded Rat brain using DNA Ligase III Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92719_rabbit-polyclonal-dna-ligase-iii-antibody-422025143048.jpg)
![Western Blot: ATG7 Antibody (3X1W4) [NBP3-15808] - Analysis of extracts from wild type(WT) and HNRNPF knockout (KD) Human cells, using HNRNPF antibody (1) at dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/ATG7-Antibody-3X1W4-Knockout-Validated-NBP3-15808-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ATG7 Antibody (3X1W4) [NBP3-15808] - Analysis of extracts from wild type(WT) and HNRNPF knockout (KD) Human cells, using HNRNPF antibody (1) at dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: ATG7 Antibody (3X1W4) [ATG7] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] ATG7 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15808_rabbit-atg7-mab-3x1w4-5220251727356.jpg)
![Immunohistochemistry: ATG7 Antibody (3X1W4) [ATG7] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using [KD Validated] ATG7 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15808_rabbit-atg7-mab-3x1w4-52202517192950.jpg)
![Immunohistochemistry: ATG7 Antibody (3X1W4) [ATG7] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KD Validated] ATG7 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15808_rabbit-atg7-mab-3x1w4-52202517213593.jpg)
![Immunohistochemistry: ATG7 Antibody (3X1W4) [ATG7] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KD Validated] ATG7 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15808_rabbit-atg7-mab-3x1w4-5220251726047.jpg)
![Immunohistochemistry: ATG7 Antibody (3X1W4) [ATG7] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] ATG7 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15808_rabbit-atg7-mab-3x1w4-52202517273554.jpg)
![Western Blot: ATG7 Antibody (3X1W4) [ATG7] - Western blot analysis of various lysates using [KD Validated] ATG7 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15808_rabbit-atg7-mab-3x1w4-52202517235726.jpg)
![Western Blot: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Western blot analysis of lysates from Mouse heart using N-Cadherin Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516334171.jpg "Western Blot: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Western blot analysis of lysates from Mouse heart using N-Cadherin Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:30s.")
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunofluorescence analysis of paraffin-embedded mouse heart using N-Cadherin Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516334199.jpg)
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunofluorescence analysis of paraffin-embedded rat heart using N-Cadherin Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-5220251639652.jpg)
![Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516283288.jpg)
![Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516334195.jpg)
![Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516293959.jpg)
![Immunohistochemistry: N-Cadherin Antibody - Azide and BSA Free [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using N-Cadherin Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-03009_rabbit-polyclonal-n-cadherin-antibody-52202516321447.jpg)