| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF816-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF8161-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-74026-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8161-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26482-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP1-77152AFP488 | |||
| Alexa Fluor Plus 555 | NBP1-77152AFP555 | |||
| Alexa Fluor Plus 594 | NBP1-77152AFP594 | |||
| Alexa Fluor Plus 647 | NBP1-77152AFP647 | |||
| Alexa Fluor Plus 680 | NBP1-77152AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77152SS | |||
![Western Blot: BAK Antibody (AT38E2) [NBP1-74026] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : A549 cell lysateLane 5 : Jurkat cell lysateLane 6 : MCF7 cell lysateLane 7 : PC3 cell lysate](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Western-Blot-NBP1-74026-img0009.jpg "Western Blot: BAK Antibody (AT38E2) [NBP1-74026] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : A549 cell lysateLane 5 : Jurkat cell lysateLane 6 : MCF7 cell lysateLane 7 : PC3 cell lysate")
![Immunocytochemistry/Immunofluorescence: BAK Antibody (AT38E2) [NBP1-74026] - Analysis of BAK1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HeLa antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Immunocytochemistry-Immunofluorescence-NBP1-74026-img0006.jpg)
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on Jurkat cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0011.jpg)
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Analysis of BAK1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0007.jpg)
![Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on RAW 246.7 cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/BAK-Antibody-AT38E2-Flow-Cytometry-NBP1-74026-img0010.jpg)
![Immunoprecipitation: BAK Antibody (8D1) [NBP3-26482] - Immunoprecipitating BAK in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26482 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26482 (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-262202413221514.jpg "Immunoprecipitation: BAK Antibody (8D1) [NBP3-26482] - Immunoprecipitating BAK in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26482 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26482 (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).")
![Immunohistochemistry: BAK Antibody (8D1) [NBP3-26482] - Image of BAK Antibody (8D1) diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-2722024840450.jpg)
![Immunohistochemistry: BAK Antibody (8D1) [NBP3-26482] - Image of BAK Antibody (8D1) diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-27220248573315.jpg)
![Western Blot: BAK Antibody (8D1) [NBP3-26482] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, Colo320 whole cell lysate. All lanes: BAK Antibody at 0.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 24, 17 KDa Observed band size: 24 KDa](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-28220241140912.jpg)
![Flow Cytometry: BAK Antibody (8D1) [NBP3-26482] - Overlay histogram showing Hela cells stained with BAK Antibody (8D1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-28220241418365.jpg)
![Western Blot: BAK Antibody [NBP1-77152] - Analysis of Bak in L1210 cell lysates with Bak antibody using NBP1-77152 at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/BAK-Antibody-Western-Blot-NBP1-77152-img0003.jpg "Western Blot: BAK Antibody [NBP1-77152] - Analysis of Bak in L1210 cell lysates with Bak antibody using NBP1-77152 at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: BAK Antibody - BSA Free [NBP1-77152] - Immunocytochemistry of BAK in L1210 cells with BAK antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77152_rabbit-polyclonal-bak-antibody-2711202419201520.jpg)
![Western Blot: BAK Antibody [NB100-893] - Staining of Human Adrenal Gland lysate (35 ug protein in RIPA buffer). BAK antibody at 2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAK-Antibody-Western-Blot-NB100-893-img0001.jpg "Western Blot: BAK Antibody [NB100-893] - Staining of Human Adrenal Gland lysate (35 ug protein in RIPA buffer). BAK antibody at 2 ug/mL. Detected by chemiluminescence.")
![ELISA: Rat BAK ELISA Kit (Colorimetric) [NBP2-69932] - Samples were spiked with high concentrations of Rat BAK and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69932_rat-bak-elisa-kit-colorimetric-132202416254029.jpg "ELISA: Rat BAK ELISA Kit (Colorimetric) [NBP2-69932] - Samples were spiked with high concentrations of Rat BAK and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat BAK ELISA Kit (Colorimetric) [NBP2-69932] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69932_rat-bak-elisa-kit-colorimetric-432024903549.jpg)
![Western Blot:[NBP3-29663]-Detection of human BAK1 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BAK1 antibody used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29663_rabbit-bak-pab-231202511585531.jpg "Western Blot:[NBP3-29663]-Detection of human BAK1 by western blot. Samples: Whole cell lysate from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BAK1 antibody used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![ELISA: Human BAK ELISA Kit (Colorimetric) [NBP2-69930] - Samples were spiked with high concentrations of Human BAK and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69930_human-bak-elisa-kit-colorimetric-132202416173450.jpg "ELISA: Human BAK ELISA Kit (Colorimetric) [NBP2-69930] - Samples were spiked with high concentrations of Human BAK and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human BAK ELISA Kit (Colorimetric) [NBP2-69930] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69930_human-bak-elisa-kit-colorimetric-4320248595164.jpg)
![ELISA: Mouse BAK ELISA Kit (Colorimetric) [NBP2-69931] - Samples were spiked with high concentrations of Mouse BAK and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69931_mouse-bak-elisa-kit-colorimetric-132202416232350.jpg "ELISA: Mouse BAK ELISA Kit (Colorimetric) [NBP2-69931] - Samples were spiked with high concentrations of Mouse BAK and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse BAK ELISA Kit (Colorimetric) [NBP2-69931] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69931_mouse-bak-elisa-kit-colorimetric-4320249151682.jpg)