Weekly Update On Happenings In The Novus Antibody Lab

Thu, 03/04/2010 - 12:10

The antibody lab technicians here at Novus Biologicals are constantly busy conducting purifications, conjugations and QC analysis in order to maintain our antibody catalog of over 100,000 products. Just this past week, the lab team conjugated numerous antibodies and tested several newly purified antibody lots.

A few recent examples of antibody conjugations include conjugating the rabbit polyclonal anti-PINK1 antibody (catalog number NB100-493) and the mouse monoclonal anti-HSD3B1 antibody (clone FDO66Q) (catalog number NB110-78644) to HRP. Horseradish peroxidase (HRP) is the most commonly used enzyme label for antibodies. Its stability, small size and broad specificity make it an ideal label for immunoblotting and immunocytochemistry. The Novus antibody lab also conjugated a large number antibodies to DyLight dyes last week, including rat monoclonal anti-CD105 antibody (clone MJ7/18) (catalog number NB100-77666) and rabbit polyclonal anti-Carbonic Anhydrase IX antibody (catalog number NB100-417).

We at Novus Biologicals adhere to a strict QC analysis process in order to assure our antibodies are validated for the appropriate research applications. Our laboratory technicians conducted numerous Western blots last week. New lots of two GRP78 antibodies (catalog numbers NBP1-06277 and NBP1-06274) were tested on HeLa whole cell lysates where specific bands at 72kDa were visualized for both antibodies. One of our newest antibodies, a LOX Propeptide antibody (catalog number NBP1-30327), was tested in Jurkat lysate and picked up the glycosylated propeptide as well as the proenzyme form. Also tested this week was a SOX2 antibody (catalog number NB110-37235). The new SOX2 antibody lot was tested on mouse brain lysate using a 4-12% MOPS gel. This Western blot analysis produced a clear band at 39kDa.

Check the Novus Biologicals Antibody Blog for frequent updates on our lab and antibody catalog, and to learn more about new antibody developments and recent scientific findings.

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