ZEB1: Regulating Organ Development and Metastasis

Thu, 08/29/2013 - 12:05

ZEB1 (Zinc finger E-box-binding homeobox 1) encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2-gene expression through binding to a small negative regulatory domain within the IL2 transcriptional start site (1). Along with its closely related family member ZEB2, ZEB1 regulates adhesion/migration genes such as E-cadherin and microRNA 200b (miR-200b) in complex developmental processes of organogenesis such as palate formation as shown by use of the ZEB1 antibody in localization profiles (2).

Western Blot: ZEB1 Antibody Western Blot: ZEB1 Antibody

Kizuka, et. al. used ZEB1 antibody in their epigenetic studies with glycans in the brain to create a combined, multilayered EMSA and ChIP profile that allowed them to identify two uniquely regulatory proteins, NeuroD1 and CTCF (3). Ryu’s group also has some interesting data with the ZEB1 antibody that suggests that aggregation of data from multiple metrics such as epithelial-mesenchymal transition (EMT; using markers such as Snail, ZEB1, E-cadherin, vimentin, and beta-Catenin) and stem cell-like phenotype (using CD44) has high value as a prognostic indicator in predicting the outcome of gastric cancers (4). Kurasawa, et. al. also used ZEB1 antibody to further investigate EMT by suggesting the role of epithelial-specific gene silencing, in a WNT7A DNA hypermethylation-dependent manner, as a stabilizing mechanism for the phenotypic plasticity of oral squamous carcinoma cells that are experiencing EMT (5).

  1. PMID: 1840704
  2. PMID: 22261924
  3. PMID: 21771782
  4. PMID: 22018628
  5. PMID: 21874048

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