The Space Between: ECM and Collagen I

Fri, 05/31/2013 - 09:44

The extracellular matrix ECM) is the material found in the extracellular environment of all tissues and organs. The composition of the extracellular framework of all vertebrates is dominated by a class of molecules known as collagens, each with unique features suited for a particular function and location.

Immunohistochemistry-Paraffin: Collagen I Antibody

Collagen proteins are composed of three subunit polypeptides that vary in length and interact to form a triple helix due to a unique repeated (Gly-x-y) sequence. Collagen I is one of the most common forms and is found in skin, tendon, vascular ligature, organs, and bone. Because of its basic and fundamental role in ECM architecture, Collagen I antibodies are helpful in identifying and/or verifying tissue-specific origin and sample tissue typing. Collagen I antibodies have been used to assess fibroblast primary sources in pulmonary fibrosis (1). Studies looking at transcriptional regulation of Procollagen Lysyl Hydroxylase 2 (PLOD2) and Hypoxia-Inducible Factor 1 (HIF-1) in breast cancer metastasis relied upon Collagen I antibodies (2,3). Collagen I antibodies helped monitor murine spleen cell differentiation in serum-free culturing conditions (4). Wound healing studies looking at liver regeneration also relied upon Collagen I antibodies (5).

  1. PMID: 14722616
  2. PMID: 23378577
  3. PMID: 23423382
  4. PMID: 20888336
  5. PMID: 23228176

Novus Biologicals is proud to offer a wide selection of Collagen reagents including antibodies, lysates, proteins, RNAi and more!

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