Sodium Potassium ATPase alpha 1 (ATP1A1): Maintaining Electrochemical Gradients

Thu, 07/18/2013 - 10:44

Sodium Potassium ATPase alpha 1 (ATP1A1) belongs to the P-type ATPase family and is the catalytic component of the active enzyme that catalyzes the hydrolysis of ATP coupled with exchange of sodium (Na+) and potassium (K+ ) ions across cellular plasma membranes. This creation of an electrochemical gradient comprised of Na+ and K+ ions provides the energy for nutrient active transport. Phosphorylation at Tyrosine 10 decreases pump activity; conversely, dephosphorylation through protein phosphatase 2A (PP2A) increases intracellular Na+ and increases catalytic activity.

Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody

Sodium potassium ATPase is key player because it provides the driving force for active Na+ as well as K+ transport into/out of the cell, with water following isosmotically. This pump consists of two main subunits: the catalytic alpha subunit (contains binding sites for cations, ATP, and cardiotonic steroid inhibitors) and regulatory beta polypeptides (essential for normal enzymatic activity). Sodium Potassium ATPase antibody has been used to demonstrate an anti-apoptotic role of the caspase-cleaved GRB-2 associated binder 1 (GAB1) docking/adaptor protein in hepatocyte growth factor/scatter factor (HGF/SF-MET) signaling (1). Other signaling studies using Sodium Potassium ATPase antibody demonstrated that endosomal endothelin-converting enzyme-1 (ECE1) regulates beta-arrestin-dependent signaling of endocytosed G-protein coupled receptors (GPCRs) through degradation of substance P (SP) in early endosomes in epithelial and neuron cells (2). Desmoulin, et. al. showed that the anti-tumor efficacies of novel antifolate bridge compounds were enhanced in tumors lacking reduced folate carrier (RFC) function in studies with Sodium Potassium ATPase antibody (3). Sodium Potassium ATPase antibody was used by Shao’s group in Pseudomonas aeruginosa lung infections (4). With the Sodium Potassium ATPase antibody they were able to show that the connective tissue ECM proteoglycan lumican plays a novel, protective role in gram-negative bacterial infections.

  1. PMID: 22915589
  2. PMID: 19531493
  3. PMID: 22740639
  4. PMID: 22865855

Novus Biologicals offers various Sodium Potassium ATPase Alpha 1 reagents for your research needs including:

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