Mannose 6 Phosphate Receptor: Once you're tagged, there's no going back!

Thu, 08/07/2014 - 13:57

Mannose 6 phosphate (M6P) is a sequence tag that plays a pivotal role in transporting proteins from the Golgi complex and cell surface to the lysosome. The M6P sequence is tagged to the N-linked oligosaccharides of lysosomal hydrolases as they traverse the cis-Golgi apparatus. Upon M6P-tagging, these proteins are targeted to the late endosome via vesicular transport. Low pH conditions there mediate the dissociation of a M6P Receptor from its ligand, allowing recycling of the M6P sequences. Furthermore, M6P is bound by lectin in the immune system, and is converted to fructose 6-phosphate by mannose phosphate isomerase.

Immunocytochemistry/Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody Immunocytochemistry/Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody

Nadimpalli et al1 used an M6P antibody to learn more about the evolutionary implications of different sized M6PR tags. While distinct from each other, both receptor forms are type I transmembrane glycoproteins and the absence of either leads to debilitating lysosomal storage diseases. Another study using an M6P antibody from Dintzis’ group at Stanford Medical School used the M6PR cation-independent antibody to evaluate their chimeric expression constructs used to study the receptor’s extracellular domains2. Their experiments indicate that those domains interfere with receptor rapid recycling between the early endosome and cell surface compartments. A more recent publication used a M6PR antibody to investigate the therapeutic effects that inhibiting M6P tagged transport of lysosomal enzymes in the brain may have on neurodegenerative disease3.

Novus Biologicals offers Mannose 6 Phosphate Receptor reagents for your research needs including:


  1. 20201808
  2. 8163521
  3. 23399673

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