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Blogs for September 2019

Isolating Immune Cells From Peripheral Blood: How and When to Use Density Gradient Centrifugation

Tuesday, September 24, 2019 - 09:05
Adaptive immunity is mediated by lymphocytes (e.g., T cells and B cells) while innate immunity responses are mediated by several cellular mediators (e.g., Neutrophils, Natural Killer Cells and Eosinophils)

By Victoria Osinski

Lymphocytes, monocytes, and granulocytes, oh my!

There are many different immune cells (leukocytes) that are found in circulation along with other cellular components such as platelets and red blood cells. Red blood cells (RBCs) are the most abundant, followed by neutrophils and lymphocytes. The immune cells can be categorized as either mononuclear cells (monocytes and lymphocytes...

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation

Tuesday, September 17, 2019 - 08:16
Expression of RhoA, B and C detected in the cytoplasm of A431 cells, a model of human epidermoid carcinoma, compared to negative control analyzed in the absence of RhoA Antibody (1A11-4G10) [NBP2-22528]

By Jamshed Arslan, Pharm. D., PhD.

The Ras homologous

Tips to Optimize Your Western Blot for Phosphorylated Protein Detection

Tuesday, September 10, 2019 - 10:48
Western blotting allows identifying and quantifying proteins from cell lysates for determination of protein expression, sub-cellular localization, post-translational modifications, protein processing, and protein-protein interactions.

By Jamshed Arslan, Pharm. D., PhD.

Protein phosphorylation refers to a reversible post-translational modification in which a protein kinase adds a phosphate group to an amino acid residue of a target protein. Protein phosphorylation, especially tyrosine phosphorylation, is one of the early events in signal transduction in all eukaryotic cells.

Once a cell is lysed, proteases and phosphatases are released that can degrade or...

The Ins and Outs of Survivin

Tuesday, September 3, 2019 - 08:40
Exosomes arise from the late endosomal compartment through inward budding of multivesicular bodies and may contain multiple proteins, lipids, DNA, RNAs and biomolecules of viruses/prions.

By Rachel M.A. Linger, Ph.D.

What is survivin?

Survivin is a small (16.5 kDa) protein normally found in human fetal tissue. In contrast, survivin is typically undetectable in most normal adult tissues. Expression of survivin occurs in several subcellular locations including the cytoplasm, nucleus,...


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