Blogs for August 2019

Chromatin reader domains of DNMT-targeting protein, UHRF1, are responsible for cancerous DNA hypermethylation

Tuesday, August 20, 2019 - 08:58
Expression of DNMT2 in human colon tissue shows cytoplasmic and nuclear staining in glandular cells, IHC

By Jamshed Arslan, Pharm. D., PhD.

DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA methylation. UHRF1 has several domains, most notably:

  • N-terminal ubiquitin-like (UBL) domain
  • TTD (tandem Tudor domain) and PHD (ubiquitin-like, containing plant homeodomain) domains that...

Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients

Tuesday, August 13, 2019 - 10:52
GFAP-green and Neurofilament-red expression in astrocytes of mouse brain cerebellum, IHC

By Michalina Hanzel, PhD

Alexander disease is a progressive and fatal neurological disease with phenotypes ranging from myelination abnormalities, gait ataxia and megalencephaly to predisposition to seizures. It is an autosomal dominant disease with mutations in the glial fibrillary acidic protein (GFAP) gene that result in astrocytic cytoplasmic inclusions, termed Rosenthal fibers, which contribute to the global neurological...

Considerations for Quantitative Western blotting

Tuesday, August 6, 2019 - 09:11
GAPDH expression in human nucleus pulposus cell lysate, WB

By Jamshed Arslan, Pharm. D., PhD.

Since its inception in 1979, Western blotting has undergone several developments. The use of radioactive probes was common throughout 1980s, but utilizing secondary antibodies labelled with fluorescent/chemiluminescent probes has virtually removed the need for radioactivity. Western blotting has become a routine quantitative method in biological research and immunodiagnostics. The following primer discusses some considerations when quantitating Western blot data.


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