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By Rosa Moreno, PhD.
This new introductory guide to methods and techniques in immunohistochemistry is written by Dr. Alexander E. Kalyuzhny, Bio-Techne, for the Springer series: Techniques in Life Science and Biomedicine for the Non-Expert . This guide provides a comprehensive introduction along with conceptual and methodological basics for new users to ensure a clear understanding upon which researchers may build their expertise in ...
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By Jamshed Arslan, Pharm. D., PhD.
DNA methylation represses transcription of many genes, including tumor suppressor genes. A protein called UHRF1 recruits DNA methyltransferases (DNMTs) to establish and maintain DNA methylation. UHRF1 has several domains, most notably:
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By Michalina Hanzel, PhD
Alexander disease is a progressive and fatal neurological disease with phenotypes ranging from myelination abnormalities, gait ataxia and megalencephaly to predisposition to seizures. It is an autosomal dominant disease with mutations in the glial fibrillary acidic protein (GFAP) gene that result in astrocytic cytoplasmic inclusions, termed Rosenthal fibers, which contribute to the global neurological...
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By Jamshed Arslan, Pharm. D., PhD.
Since its inception in 1979, Western blotting has undergone several developments. The use of radioactive probes was common throughout 1980s, but utilizing secondary antibodies labelled with fluorescent/chemiluminescent probes has virtually removed the need for radioactivity. Western blotting has become a routine quantitative method in biological research and immunodiagnostics. The following primer discusses some considerations when quantitating Western blot data.
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