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Blogs for May 2019

Repurposing FDA-approved drugs to combat the rise of antibiotic resistance

Tuesday, May 28, 2019 - 09:03
Actin staining with Phalloidin with VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium, Novus Biologicals

By Beth Melson, MS

Antibiotic resistance is a global threat to public health. Widespread, inappropriate use of antibiotics, such as to treat viral infections or promote growth in livestock, has led to increased incidence of antibiotic resistance1. Resistance is costly to both human health as well as the economy1, and thus the identification of new, effective antibiotics is a public health priority. One solution to this problem is to repurpose commercially available, FDA-approved drugs to target host cell processes that would...

Mechanisms of Neurodegeneration: Mitochondrial Dysfunction and Oxidative Stress

Tuesday, May 21, 2019 - 07:44
3D image of dopaminergic neurons shown in green, Bio-Techne.

By Michalina Hanzel, PhD

In this second installment of our three blog-posts series on major cellular mechanisms responsible for neurodegenerative disorders, we will explore the processes of mitochondrial dysfunction and oxidative stress. Previously, the role of autophagy in the clearance of aggregation-prone proteins in the context of neurodegenerative diseases was discussed in Mechanisms of Neurodegeneration:...

Toll-like receptor 2 activation contributes to oral squamous cell carcinoma development and miRNA-mediated drug resistance

Tuesday, May 14, 2019 - 08:56
Nuclear Factor kappa B (NFkB) signaling pathway poster, Novus Biologicals

By Jamshed Arslan, Pharm. D., PhD.

Squamous cell carcinoma is the most common cancer in the oral cavity.1 The tumor surface biofilms in oral cancers contain high levels of aerobic and anaerobic microorganisms.1,2 Periodontal pathogens can activate epithelial Toll-like receptors (TLRs) on tumor cells to promote oral squamous cell carcinoma (OSCC) progression. In this regard, TLR2 is known to recognize micro-organisms in the oral cavity by forming a heterodimer with TLR1 or TLR6...

Make each cell count: How to assess autophagy using flow cytometry

Tuesday, May 7, 2019 - 09:32
LC3B expression in HeLa cells treated with chloroquine overnight, ICC. Novus Biologicals

Kristy R. Howell, PhD

The cellular recycling process known as autophagy may be induced by a variety of conditions including reduced nutrient availability, serum starvation and pharmacological agents (e.g., Rapamycin (mTOR)). Upon autophagy induction, a process of increased sequestration of dysfunctional cellular components into autophagosomes ensues. The autophagosome fuses...


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