Blogs for February 2019

Wet or dry protein transfer: Which one to choose for your western blotting?

Tuesday, February 12, 2019 - 10:50
Novus Biologicals western blot resources page

By Jamshed Arslan, Pharm. D., PhD.

Western blotting involves three steps: 1) separating proteins by size through gel electrophoresis 2) transferring separated proteins to a solid support (typically, a PVDF membrane) and 3) using antibody to mark your protein(s) of interest in the mixture of proteins. The second step of moving negatively charged proteins from the gel to a blotting membrane (PVDF) can be performed in dry or wet conditions.

Dry/semi-dry transfers

Dry conditions are famous for easiness and speed. Reagents, power packs and wiring involved in dry transfers are less messy and therefore, potentially less hazardous. They allow blotting of several...

Animal Models to Study Autophagy

Tuesday, February 5, 2019 - 09:03
Autophagy Signaling Pathway, Novus Biologicals

By Christina Towers, PhD

What is autophagy?

Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. In the beginning... the process of macroautophagy was originally characterized in yeast where the core autophagy proteins or Apgs were identified. In this first system, the multistep process of phagophore initiation and elongation as well as the conjugation machinery that mediate efficient autophagosome completion were determined and cloned to assess function. The mechanisms of starvation induced autophagy as well as many of the selective autophagy processes were also hashed out in...

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