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![Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Western Blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Lane 5: Mouse IgM. Load: 5 ng per lane.Primary antibody: Mouse IgG2a antibody at 1 ug/mL for overnight at 4C.Secondary antibody: Gt-anti-Rabbit HRP at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 50 kDa and25 kDa, 50 kDa and 25 kDa for Mouse IgG2a. Other band(s): Weak reactivity for to Mouse IgG1.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72794-img0010.jpg "Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Western Blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Lane 5: Mouse IgM. Load: 5 ng per lane.Primary antibody: Mouse IgG2a antibody at 1 ug/mL for overnight at 4C.Secondary antibody: Gt-anti-Rabbit HRP at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 50 kDa and25 kDa, 50 kDa and 25 kDa for Mouse IgG2a. Other band(s): Weak reactivity for to Mouse IgG1.")
![Flow Cytometry: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Antibody [DyLight 405] [NBP1-72921]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Flow-Cytometry-NBP1-72794-img0005.jpg)
![Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Lane 1: Mouse IgG2a. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG2a Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: incubated with blocking buffer for 30 min at RT. Reaction visualized with alkaline phosphatase substrate for 30 seconds at RT. Predicted/Observed size: 55kDa for Mouse IgG2a.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP1-72794-img0003.jpg)
![Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Mouse IgG2a Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4 Lane 2.5ng](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72794-img0004.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Rabbit anti-Mouse IgG2a Antibody [DyLight 800] [NBP1-72915]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP1-72794-img0006.jpg)
![Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Secondary Antibody [DyLight 488] [NBP1-72891] Image from the DyLight 488 version of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Fluorophore-linked-immunosorbent-assay-NBP1-72794-img0008.jpg)
![Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG2a-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72794-img0009.jpg)
