IgG2A Antibodies

MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (Catalog # MAB5718, Mouse IgG₁isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG₃isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG_2A(Catalog # MAB0033) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG_2Aisotype antibody at approximately 18 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) but not Rabbit Anti-Mouse IgG_2AMonoclonal Antibody (Catalog # MAB0033). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (Catalog # MAB5718, Mouse IgG₁isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG₃isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG_2A(Catalog # MAB0033) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG_2Aisotype antibody at approximately 18 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) but not Rabbit Anti-Mouse IgG_2AMonoclonal Antibody (Catalog # MAB0033). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (Catalog # MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (Catalog # MAB5718, Mouse IgG₁isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG₃isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG_2A(Catalog # MAB0033) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG_2Aisotype antibody at approximately 18 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) but not Rabbit Anti-Mouse IgG_2AMonoclonal Antibody (Catalog # MAB0033). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB100, Mouse IgG₁isotype), Mouse anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG_2Aisotype), or Mouse anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG_2Bisotype), followed by Rabbit anti-Mouse IgG_2A-specific secondary antibody (Catalog # MAB0033) then PE-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB100, Mouse IgG₁isotype), Mouse anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG_2Aisotype), or Mouse anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG_2Bisotype), followed by Rabbit anti-Mouse IgG_2A-specific secondary antibody (Catalog # MAB0033) then PE-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8 alpha Monoclonal Antibody(Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB0041, open histogram), followed by Rat Anti-Mouse IgG2A isotype-specific secondary antibody (Catalog # MAB0032) and then Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8 alpha Monoclonal Antibody(Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB0041, open histogram), followed by Rat Anti-Mouse IgG2A isotype-specific secondary antibody (Catalog # MAB0032) and then Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8 alpha Monoclonal Antibody(Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB0041, open histogram), followed by Rat Anti-Mouse IgG2A isotype-specific secondary antibody (Catalog # MAB0032) and then Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />