Rat anti-Mouse IgG2a Secondary Antibody (344701) [Allophycocyanin]

Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB003, open histogram), followed by APC-conjugated Rat Anti-Mouse IgG2AIsotype-specific Secondary Antibody (Catalog # F0130). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 344701
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

Rat anti-Mouse IgG2a Secondary Antibody (344701) [Allophycocyanin] Summary

• Immunogen: Cocktail of mouse IgG₁ and IgG_2A
• Specificity: The specificity of this antibody was confirmed by direct ELISA on mouse IgG 2A monoclonal antibodies, and it does not cross-react with IgG 1 IgG 2B IgG 3 IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG 2A isotype, and does not detect primary antibodies of mouse IgG 1 IgG 2B or IgG 3 class.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  12 months from date of receipt, 2 to 8 degreesC as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat anti-Mouse IgG2a Secondary Antibody (344701) [Allophycocyanin]

• IgG2A

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG2a Antibody (F0130)(2)

Publications using F0130

• Wu, F;Han, Y;Xiong, Q;Tang, H;Shi, J;Yang, Q;Li, X;Jia, H;Qian, J;Dong, Y;Li, T;Gao, Y;Qian, Z;Wang, H;Wang, T; Cerebral Endothelial CXCR2 Promotes Neutrophil Transmigration into Central Nervous System in LPS-Induced Septic Encephalopathy Biomedicines 2024-07-11 [PMID: 39062109] (Flow Cytometry Control, Mouse, Transgenic Mouse)
• EMMA Andriessen, F Binet, F Fournier, M Hata, A Dejda, G Mawambo, S Crespo-Gar, F Pilon, M Buscarlet, K Beauchemin, V Bougie, G Cumberlidg, AM Wilson, S Bourgault, FA Rezende, N Beaulieu, JS Delisle, P Sapieha Myeloid-resident neuropilin-1 promotes choroidal neovascularization while mitigating inflammation Embo Molecular Medicine, 2021-04-19;0(0):e11754. 2021-04-19 [PMID: 33876574] (Flow Cytometry, Mouse)