Sheep anti-Mouse IgG2a Secondary Antibody

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Sheep anti-Mouse IgG2a Secondary Antibody Summary

• Description: The lyophilized antiserum is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.
• Immunogen: Highly purified normal homogenous IgG2a(1b) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the subclass IgG2a. It reacts strongly withIg2a of the allelic types Igh-1a and Igh-1b, which include BALB/C, C57BL, CBA/J, SJ/J and SM/J. When used for the identification of IgG2a in other mouse strains the reaction may be weaker. The antiserum contains antibodies to iso and allospecific determinants. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
• Clonality: Polyclonal
• Host: Sheep
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • Immunoelectrophoresis
• Application Notes: Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter center well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells.
  
  Precipitation assays.

Reactivity Notes

The antiserum does not cross-react with any other component of the Mouse Ig system. Inter-species crossreactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail; expected chance of cross-reactivity will be small.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Whole Antiserum
• Reconstitution Instructions: Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Alternate Names for Sheep anti-Mouse IgG2a Secondary Antibody

• IgG2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.