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![Western Blot: NMDAR2A Antibody (S327-95) [NBP2-22405] - Western Blot analysis of Monkey COS cells transfected with GFP-tagged NMDAR2A showing detection of ~170 kDa NMDAR2A protein using Mouse Anti-NMDAR2A Monoclonal Antibody, Clone S327-95 (NBP2-22405). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NMDAR2A. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NMDAR2A Monoclonal Antibody (NBP2-22405) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-S327-95-Western-Blot-NBP2-22405-img0003.jpg "Western Blot: NMDAR2A Antibody (S327-95) [NBP2-22405] - Western Blot analysis of Monkey COS cells transfected with GFP-tagged NMDAR2A showing detection of ~170 kDa NMDAR2A protein using Mouse Anti-NMDAR2A Monoclonal Antibody, Clone S327-95 (NBP2-22405). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NMDAR2A. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NMDAR2A Monoclonal Antibody (NBP2-22405) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.")
![Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327-95 (NBP2-22405). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (NBP2-22405) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2A/NR2A Antibody (D) Composite.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-S327-95-Immunocytochemistry-Immunofluorescence-NBP2-22405-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-S327-95-Immunocytochemistry-Immunofluorescence-NBP2-22405-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327-95 (NBP2-22405). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (NBP2-22405) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN2A/NR2A Antibody. (D) Composite.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-S327-95-Immunocytochemistry-Immunofluorescence-NBP2-22405-img0005.jpg)
![Western Blot: NMDAR2A Antibody (S327A-38) [NBP2-22404] - Western Blot analysis of Monkey COS cells transfected with GFP-tagged NMDAR2A showing detection of ~170 kDa NMDAR2A protein using Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody, Clone S327A-38 (NBP2-22404). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NMDAR2A. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NMDAR2A Monoclonal Antibody (NBP2-22404) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-S327A-38-Western-Blot-NBP2-22404-img0002.jpg "Western Blot: NMDAR2A Antibody (S327A-38) [NBP2-22404] - Western Blot analysis of Monkey COS cells transfected with GFP-tagged NMDAR2A showing detection of ~170 kDa NMDAR2A protein using Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody, Clone S327A-38 (NBP2-22404). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NMDAR2A. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NMDAR2A Monoclonal Antibody (NBP2-22404) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa.")
![Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody, Clone S327A-38 (NBP2-22404). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody (NBP2-22404) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2A/NMDAR2A Antibody (D) Composite.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-S327A-38-Immunocytochemistry-Immunofluorescence-NBP2-22404-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327A-38) [NBP2-22404] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody, Clone S327A-38 (NBP2-22404). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NMDAR2A Monoclonal Antibody (NBP2-22404) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasm. Magnification: 60X. (A) Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. (B) Anti-GluN2A/NMDAR2A Antibody. (C) Composite. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN2A/NMDAR2A Antibody. (D) Composite.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-S327A-38-Immunocytochemistry-Immunofluorescence-NBP2-22404-img0004.jpg)
![Western Blot: NMDAR2A Antibody [NBP3-10351] - WB Suggested Anti-NMDAR2A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle](http://images.novusbio.com/fullsize/NMDAR2A Antibody-Western Blot-NBP3-10351-img0001.jpg "Western Blot: NMDAR2A Antibody [NBP3-10351] - WB Suggested Anti-NMDAR2A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle")