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![Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] - Image of LEF1 Antibody (13B7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.](http://images.novusbio.com/fullsize/nbp3-26171_rabbit-lef1-mab-13b7-26220241547562.jpg "Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] - Image of LEF1 Antibody (13B7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.")
![Immunohistochemistry: LEF1 Antibody (13B7) [NBP3-26171] - Image of LEF1 Antibody (13B7) diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.](http://images.novusbio.com/fullsize/nbp3-26171_rabbit-lef1-mab-13b7-26220241535579.jpg)
![Immunoprecipitation: NFATC3/NFAT4 Antibody [NBP2-04025] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04025 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04026 and NBP2-04027, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/NFATC3-NFAT4-Antibody-Immunoprecipitation-NBP2-04025-img0002.jpg "Immunoprecipitation: NFATC3/NFAT4 Antibody [NBP2-04025] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04025 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04026 and NBP2-04027, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
![Western Blot: RBX1 Antibody (C5) [NBP3-42105] - Sample: Lane1: Rat Thyroid lysate; Lane2: Porcine Liver lysate; Lane3: Porcine Skin lysate; Lane4: A549 cell lysate Primary Ab: 2ug/ml NBP3-42105; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42105_mouse-rbx1-mab-c5-61220242058857.jpg "Western Blot: RBX1 Antibody (C5) [NBP3-42105] - Sample: Lane1: Rat Thyroid lysate; Lane2: Porcine Liver lysate; Lane3: Porcine Skin lysate; Lane4: A549 cell lysate Primary Ab: 2ug/ml NBP3-42105; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: Wnt-5a Antibody (C3) [NBP3-42192] - Sample: Recombinant Wnt-5a, Mouse.](http://images.novusbio.com/fullsize/nbp3-42192_mouse-wnt-5a-mab-c3-612202421373031.jpg "Western Blot: Wnt-5a Antibody (C3) [NBP3-42192] - Sample: Recombinant Wnt-5a, Mouse.")
![Western Blot: Wnt-5a Antibody (C3) [NBP3-42192] - Sample: Rat Liver lysate Primary Ab: 2ug/ml NBP3-42192; Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42192_mouse-wnt-5a-mab-c3-612202421175922.jpg)