| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94468-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37451-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98877-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06478-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88961-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26414-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26422-50ul | |||
![Western Blot: Wnt-2 Antibody [NBP2-94468] - Rat kidney using WNT2 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-94468_rabbit-polyclonal-wnt-2-antibody-immunohistochemistry-paraffin-145202413540..jpg "Western Blot: Wnt-2 Antibody [NBP2-94468] - Rat kidney using WNT2 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Western Blot: Wnt-2 Antibody [NBP2-94468] - analysis of various lysates, using WNT2 antibody at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/antibody/nbp2-94468_rabbit-polyclonal-wnt-2-antibody-western-blot-14520241375..jpg)
![Western Blot: JNK1/2/3 Antibody (0J5D8) [NBP3-16250] - Western blot analysis of extracts of various cell lines, using JNK1/2/3 Rabbit mAb (NBP3-16250) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/JNK1-2-3-Antibody-0J5D8-Western-Blot-NBP3-16250-img0002.jpg "Western Blot: JNK1/2/3 Antibody (0J5D8) [NBP3-16250] - Western blot analysis of extracts of various cell lines, using JNK1/2/3 Rabbit mAb (NBP3-16250) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry: JNK1/2/3 Antibody (0J5D8) [NBP3-16250] - Immunofluorescence analysis of mouse brain using JNK1/2/3 Rabbit mAb (NBP3-16250) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/JNK1-2-3-Antibody-0J5D8-Immunohistochemistry-NBP3-16250-img0001.jpg)
![Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734] - Western blot analysis of extracts of various cell lines, using Skp1 Rabbit mAb (NBP3-16734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Skp1-Antibody-10G5X5-Western-Blot-NBP3-16734-img0002.jpg "Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734] - Western blot analysis of extracts of various cell lines, using Skp1 Rabbit mAb (NBP3-16734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (10G5X5) [NBP3-16734] - Confocal imaging of NIH/3T3 cells using Skp1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16734_rabbit-skp1-mab-10g5x5-822025201048.jpg)
![Western Blot: ROCK2 Antibody (8G3U2) [NBP3-16123] - Western blot analysis of extracts of various cell lines, using ROCK2 Rabbit mAb (NBP3-16123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/ROCK2-Antibody-8G3U2-Western-Blot-NBP3-16123-img0002.jpg "Western Blot: ROCK2 Antibody (8G3U2) [NBP3-16123] - Western blot analysis of extracts of various cell lines, using ROCK2 Rabbit mAb (NBP3-16123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: ROCK2 Antibody (8G3U2) [NBP3-16123] - Analysis of paraffin-embedded Rat colon tissue using ROCK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16123_rabbit-rock2-mab-8g3u2-immunohistochemistry-paraffin-71202584338..png)
![Immunohistochemistry-Paraffin: ROCK2 Antibody (8G3U2) [NBP3-16123] - Analysis of paraffin-embedded Mouse intestin tissue using ROCK2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16123_rabbit-rock2-mab-8g3u2-immunohistochemistry-paraffin-71202584440..png)
![Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mouse mAb against A549 (1), and PC-3 (2) cell lysate.](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Western-Blot-NBP2-37451-img0003.jpg "Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mouse mAb against A549 (1), and PC-3 (2) cell lysate.")
![Flow Cytometry: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis of HepG2 cells using FZD5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Flow-Cytometry-NBP2-37451-img0004.jpg)
![Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mAb against human FZD5 recombinant protein. (Expected MW is 32.5 kDa).](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Western-Blot-NBP2-37451-img0002.jpg)
![ELISA: Frizzled-5 Antibody (2D12) [NBP2-37451] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-ELISA-NBP2-37451-img0001.jpg)
![Western Blot: WIF-1 Antibody (1G5) [NBP1-51658] - Analysis using WIF1 mouse mAb against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.](http://images.novusbio.com/fullsize/WIF-1-Antibody-1G5-Western-Blot-NBP1-51658-img0006.jpg "Western Blot: WIF-1 Antibody (1G5) [NBP1-51658] - Analysis using WIF1 mouse mAb against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: WIF-1 Antibody (1G5) [NBP1-51658] - Analysis of Hela cells using WIF1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/WIF-1-Antibody-1G5-Immunocytochemistry-Immunofluorescence-NBP1-51658-img0007.jpg)
![Immunohistochemistry-Paraffin: WIF-1 Antibody (1G5) [NBP1-51658] - Analysis of ovary tumour tissues (left) and lung cancer (right) using WIF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/WIF-1-Antibody-1G5-Immunohistochemistry-Paraffin-NBP1-51658-img0005.jpg)
![Western Blot: Cyclin D3 Antibody [NBP2-98877] - Anti-Cyclin D3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa](http://images.novusbio.com/fullsize/Cyclin-D3-Antibody-Western-Blot-NBP2-98877-img0002.jpg "Western Blot: Cyclin D3 Antibody [NBP2-98877] - Anti-Cyclin D3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 33 kDa")
![Immunocytochemistry/Immunofluorescence: Cyclin D3 Antibody [NBP2-98877] - Immunofluorescence staining of Cyclin D3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98877 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to nucleus and cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98877_rabbit-polyclonal-cyclin-d3-antibody-23520251125446.png)
![Western Blot: PPP2R5E Antibody [NBP3-06478] - Lane A: HeLa Whole Cell LysateLane B: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:53 kDa](http://images.novusbio.com/fullsize/PPP2R5E-Antibody-Western-Blot-NBP3-06478-img0001.jpg "Western Blot: PPP2R5E Antibody [NBP3-06478] - Lane A: HeLa Whole Cell LysateLane B: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:53 kDa")
![Immunohistochemistry-Paraffin: PPP2R5E Antibody [NBP3-06478] - Staining of human PPP2R5E in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PPP2R5E-Antibody-Immunohistochemistry-Paraffin-NBP3-06478-img0002.jpg)
![Immunoprecipitation: PPP2R5E Antibody [NBP3-06478] - Lane A:0.5 mg 293 Whole Cell Lysate4 uL anti-PPP2R5E rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PPP2R5E rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :53 kDa](http://images.novusbio.com/fullsize/PPP2R5E-Antibody-Immunoprecipitation-NBP3-06478-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Immunocytochemistry analysis of A431 cells labeling PPP2R5D with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-2920241192344.jpg "Immunocytochemistry/ Immunofluorescence: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Immunocytochemistry analysis of A431 cells labeling PPP2R5D with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Flow cytometric analysis of A431 cells labeling PPP2R5D.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32846, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-29202416372164.jpg)
![Western blot analysis on different lysates with NBP3-32846 at 1/1,000 dilution. Lane 1: A431 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: MCF7 cell lysate (20 µg/Lane) Lane 5: 293T cell lysate (20 µg/Lane) Lane 6: Jurkat cell lysate (20 µg/Lane) Lane 7: Human brain tissue lysate (40 µg/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32846 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32846_-western-blot-639204595240481208.jpg)
![Western blot analysis on different lysates with NBP3-32846 at 1/1,000 dilution. Lane 1: Human PPP2R5C recombinant protein aa (aa1-524) 50ng Lane 2: Human PPP2R5D recombinant protein aa (aa107-602) 50ng Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32846 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32846_-western-blot-639204598084275099.jpg)
![Western Blot: PPP2R5C Antibody [NBP1-88961] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Western-Blot-NBP1-88961-img0005.jpg "Western Blot: PPP2R5C Antibody [NBP1-88961] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: PPP2R5C Antibody [NBP1-88961] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/PPP2R5C-Antibody-Immunohistochemistry-Paraffin-NBP1-88961-img0002.jpg)
![Immunohistochemistry: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Image of c-jun [p Ser63] Antibody (4A11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-2722024850463.jpg "Immunohistochemistry: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Image of c-jun [p Ser63] Antibody (4A11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![<b>Biological Strategies Validation. </b>Western Blot: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate (treated with Calyculin A or EGF). All lanes: c-jun [p Ser63] Antibody at 0.95 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48 KDa Observed band size: 48 KDa](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-282202411472127.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Staining of A549 cells with c-jun [p Ser63] Antibody (4A11) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-28220241312248.jpg)
![Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-26220241525330.jpg "Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![<b>Biological Strategies Validation. </b>Western Blot: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate (treated with Calyculin A or not). All lanes: GSK-3 beta [p Ser9] Antibody at 0.77ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46 KDa Observed band size: 46 KDa](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241136300.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Staining of Hela cells(treated with 50mM Calyculin A for 30min) with GSK-3 beta [p Ser9] Antibody (3A6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241312216.jpg)