| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93815-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38201-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93680-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37989-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37510-0.025ml | |||
![Western Blot: PRICKLE1 Antibody - BSA Free [NBP2-81771] - Western blot analysis of PRICKLE1 in human bladder tissue lysate with PRICKLE1 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81771_rabbit-polyclonal-prickle1-antibody-271120242013154.gif "Western Blot: PRICKLE1 Antibody - BSA Free [NBP2-81771] - Western blot analysis of PRICKLE1 in human bladder tissue lysate with PRICKLE1 antibody at 1 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: PRICKLE1 Antibody - BSA Free [NBP2-81771] - Immunofluorescence of PRICKLE1 in human bladder cells with PRICKLE1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81771_rabbit-polyclonal-prickle1-antibody-271120242042518.jpg)
![Western Blot: Phospholipase C beta 2 Antibody [NBP2-93815] - Western blot analysis of extracts of Rat lung, using Phospholipase C beta 2 antibody (NBP2-93815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Phospholipase-C-beta-2-Antibody-Western-Blot-NBP2-93815-img0001.jpg "Western Blot: Phospholipase C beta 2 Antibody [NBP2-93815] - Western blot analysis of extracts of Rat lung, using Phospholipase C beta 2 antibody (NBP2-93815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: Phospholipase C beta 2 Antibody [NBP2-93815] - Western blot analysis of extracts of HEL cells, using Phospholipase C beta 2 antibody (NBP2-93815) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Phospholipase C beta 2 Antibody-Western Blot-NBP2-93815-img0002.jpg)
![Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734] - Western blot analysis of extracts of various cell lines, using Skp1 Rabbit mAb (NBP3-16734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Skp1-Antibody-10G5X5-Western-Blot-NBP3-16734-img0002.jpg "Western Blot: Skp1 Antibody (10G5X5) [NBP3-16734] - Western blot analysis of extracts of various cell lines, using Skp1 Rabbit mAb (NBP3-16734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: Skp1 Antibody (10G5X5) [NBP3-16734] - Confocal imaging of NIH/3T3 cells using Skp1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16734_rabbit-skp1-mab-10g5x5-822025201048.jpg)
![Western Blot: PKC beta Antibody [NBP3-38201] - Western blot analysis of various lysates using PKC beta Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38201_rabbit-pkc-beta-pab-912202414112810.jpg "Western Blot: PKC beta Antibody [NBP3-38201] - Western blot analysis of various lysates using PKC beta Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP3-38201] - Immunofluorescence analysis of NIH/3T3 cells using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38201_rabbit-pkc-beta-pab-3112025177029.jpg)
![Western Blot: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-2E2-Western-Blot-NBP1-30165-img0002.jpg "Western Blot: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Cell lysates of HeLa and MCF7 (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Casein Kinase 1 alpha (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Analysis of Casein Kinase 1 alpha in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Casein Kinase 1 alpha antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-2E2-Immunocytochemistry-Immunofluorescence-NBP1-30165-img0006.jpg)
![Western Blot: Casein Kinase 1 alpha Antibody (2E2) [NBP1-30165] - Lane 1.: Jurkat cell lysate. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Caseine kinase 1 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-2E2-Western-Blot-NBP1-30165-img0003.jpg)
![Western Blot: Casein Kinase 1 alpha Antibody (9O4L9) [NBP3-16807] - Western blot analysis of extracts of various cell lines, using Casein Kinase 1 alpha (Casein Kinase 1 alpha) Rabbit mAb (NBP3-16807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-9O4L9-Western-Blot-NBP3-16807-img0002.jpg "Western Blot: Casein Kinase 1 alpha Antibody (9O4L9) [NBP3-16807] - Western blot analysis of extracts of various cell lines, using Casein Kinase 1 alpha (Casein Kinase 1 alpha) Rabbit mAb (NBP3-16807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Casein Kinase 1 alpha Antibody (9O4L9) [NBP3-16807] - Immunohistochemistry of paraffin-embedded rat liver using Casein Kinase 1 alpha (Casein Kinase 1 alpha) Rabbit mAb (NBP3-16807) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Casein-Kinase-1-alpha-Antibody-9O4L9-Immunohistochemistry-Paraffin-NBP3-16807-img0001.jpg)
![Western Blot: PLC-beta 1 Antibody - BSA Free [PLC-beta 1] - Western blot analysis of lysates from HeLa cells using PLC-beta 1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp2-93680_rabbit-polyclonal-plc-beta-1-antibody-5220251628081.jpg "Western Blot: PLC-beta 1 Antibody - BSA Free [PLC-beta 1] - Western blot analysis of lysates from HeLa cells using PLC-beta 1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Immunohistochemistry-Paraffin: PLC-beta 1 Antibody [NBP2-93680] - Paraffin-embedded rat brain using PLC-beta 1 .](http://images.novusbio.com/fullsize/PLC-beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-93680-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: CTBP2 Antibody [NBP3-37989] - Immunofluorescence analysis of U2OS cells using CTBP2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37989_rabbit-ctbp2-pab-10122024121033.jpg "Immunocytochemistry/ Immunofluorescence: CTBP2 Antibody [NBP3-37989] - Immunofluorescence analysis of U2OS cells using CTBP2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: CTBP2 Antibody [NBP3-37989] - Western blot analysis of various lysates using CTBP2 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-37989_rabbit-ctbp2-pab-101220241233858.jpg)
![Western Blot: LRP-5 Antibody (2B11) [NBP2-37510] - Western blot analysis using LRP5 mAb against human LRP5 (AA: 1422-1615) recombinant protein. (Expected MW is 20.8 kDa)](http://images.novusbio.com/fullsize/LRP-5-Antibody-2B11-Western-Blot-NBP2-37510-img0002.jpg "Western Blot: LRP-5 Antibody (2B11) [NBP2-37510] - Western blot analysis using LRP5 mAb against human LRP5 (AA: 1422-1615) recombinant protein. (Expected MW is 20.8 kDa)")
![Flow Cytometry: LRP-5 Antibody (2B11) [NBP2-37510] - Flow cytometric analysis of HeLa cells using LRP5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/LRP-5-Antibody-2B11-Flow-Cytometry-NBP2-37510-img0003.jpg)
![ELISA: LRP-5 Antibody (2B11) [NBP2-37510] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/LRP-5-Antibody-2B11-ELISA-NBP2-37510-img0001.jpg)
![Western Blot: PPP2R5A Antibody [NBP3-30774] - Legend: Novus Biologicals' sheep anti PP 2 A/B alpha western blot of total rat brain homogenate.](http://images.novusbio.com/fullsize/nbp3-30774_sheep-ppp2r5a-pab-81120242059218.jpg "Western Blot: PPP2R5A Antibody [NBP3-30774] - Legend: Novus Biologicals' sheep anti PP 2 A/B alpha western blot of total rat brain homogenate.")
![Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-28820241246162.jpg "Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Immunocytochemistry analysis of A549 cells labeling SIAH1 with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-29202410234222.jpg)
![Western Blot: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Human brain tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32846) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-288202412442968.jpg "Western Blot: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Human brain tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32846) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Immunocytochemistry analysis of A431 cells labeling PPP2R5D with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-2920241192344.jpg)
![Flow Cytometry: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Flow cytometric analysis of A431 cells labeling PPP2R5D. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32846, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-29202416372164.jpg)