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![Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0002.jpg "Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Untreated (-) and treated (+) Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0001.jpg)
![ELISA: Human S100A9 ELISA Kit (Colorimetric) [NBP3-06955] - Samples were spiked with high concentrations of Human S100A9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06955_human-s100a9-elisa-kit-colorimetric-1322024168147.jpg "ELISA: Human S100A9 ELISA Kit (Colorimetric) [NBP3-06955] - Samples were spiked with high concentrations of Human S100A9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human S100A9 ELISA Kit (Colorimetric) [NBP3-06955] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06955_human-s100a9-elisa-kit-colorimetric-4320248595132.jpg)
![Flow Cytometry: TCR alpha Antibody (F1) [NBP2-22493] - Analysis of FITC conjugate of NBP2-22493. Flow cytometry TCR V alpha 2 (F1) on TCR V alpha 2 positive MOLT16 cells (left panel) or negative control HPB-ALL cells (right panel). Equal numbers of cells were stained with a FITC labeled TCR V alpha 2 (F1) mon](http://images.novusbio.com/fullsize/TCR-alpha-Antibody-F1-Flow-Cytometry-NBP2-22493-img0001.jpg "Flow Cytometry: TCR alpha Antibody (F1) [NBP2-22493] - Analysis of FITC conjugate of NBP2-22493. Flow cytometry TCR V alpha 2 (F1) on TCR V alpha 2 positive MOLT16 cells (left panel) or negative control HPB-ALL cells (right panel). Equal numbers of cells were stained with a FITC labeled TCR V alpha 2 (F1) mon")
![Western Blot: Insulin R/CD220 Antibody [NBP1-74140] - Titration: 1.0 ug/ml Positive Control: Mouse Pancreas.](http://images.novusbio.com/fullsize/Insulin-R-CD220-Antibody-Western-Blot-NBP1-74140-img0003.jpg "Western Blot: Insulin R/CD220 Antibody [NBP1-74140] - Titration: 1.0 ug/ml Positive Control: Mouse Pancreas.")
![Western Blot: YAP1 [p Ser128] Antibody [NBP3-16939] - Western blot analysis of extracts of NIH/3T3 cells, using YAP1 antibody (NBP3-16939) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/YAP1-[p-Ser128]-Antibody-Western-Blot-NBP3-16939-img0001.jpg "Western Blot: YAP1 [p Ser128] Antibody [NBP3-16939] - Western blot analysis of extracts of NIH/3T3 cells, using YAP1 antibody (NBP3-16939) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.")
![Western Blot: YAP1 [p Ser128] Antibody - Azide and BSA Free [YAP1] - Western blot analysis of lysates from HeLa cells using YAP1 Rabbit pAb at 1:2000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16939_rabbit-yap1-p-ser-128-pab-52202517352786.jpg)
![Western Blot: VEGFR1/Flt-1 Antibody (7F1A3) [NBP3-15683] - Western blot analysis of extracts of various cell lines, using VEGFR1/Flt-1 antibody (NBP3-15683) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-7F1A3-Western-Blot-NBP3-15683-img0001.jpg "Western Blot: VEGFR1/Flt-1 Antibody (7F1A3) [NBP3-15683] - Western blot analysis of extracts of various cell lines, using VEGFR1/Flt-1 antibody (NBP3-15683) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: VEGFR1/Flt-1 Antibody (7F1A3) [NBP3-15683] - Western blot analysis of extracts of various cell lines, using VEGFR1/Flt-1 antibody (NBP3-15683) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/VEGFR1-Flt-1-Antibody-7F1A3-Western-Blot-NBP3-15683-img0002.jpg)
![Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.](http://images.novusbio.com/fullsize/MEK1-[p-Ser218-p-Ser222]-Antibody-Western-Blot-NB500-139-img0001.jpg "Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NB500-139] - Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.")
![Western Blot: YAP1 Antibody (S06-5E9) [NBP3-19805] - Western blot detection of YAP1 in Hela cell lysates using NBP3-19805 (1:1000 diluted). Predicted band size: 55kDa. Observed band size: 70-75kDa.](http://images.novusbio.com/fullsize/YAP1-Antibody-S06-5E9-Western-Blot-NBP3-19805-img0001.jpg "Western Blot: YAP1 Antibody (S06-5E9) [NBP3-19805] - Western blot detection of YAP1 in Hela cell lysates using NBP3-19805 (1:1000 diluted). Predicted band size: 55kDa. Observed band size: 70-75kDa.")
![Western Blot: SHP-2/PTPN11 [p Tyr 542] Antibody (SR2126) [NBP3-21539] - Western blot analysis using NBP3-21539 in (1) NIH/3T3 cell lysates; (2) NIH/3T3 cell lysates treated with PDGF.](http://images.novusbio.com/fullsize/nbp3-21539_rabbit-shp-2-ptpn11-p-tyr-542-mab-sr2126-672023106217.jpg "Western Blot: SHP-2/PTPN11 [p Tyr 542] Antibody (SR2126) [NBP3-21539] - Western blot analysis using NBP3-21539 in (1) NIH/3T3 cell lysates; (2) NIH/3T3 cell lysates treated with PDGF.")