| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50169-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50408-0.025ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP2-22494 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16939-20ul | |||
![Western Blot: HGF R/c-MET Antibody (12.1) [NBP2-50169] - Endogenous c-Met expression in a panel of human gastric cell lines. mAb12.1-HRP recognises 190kD pro-s-Met and 50kD mature c-Met alpha chain. mAb 12.1-HRP does not recognise the 145kD mature c-Met beta-chain.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-12-1-Western-Blot-NBP2-50169-img0001.jpg "Western Blot: HGF R/c-MET Antibody (12.1) [NBP2-50169] - Endogenous c-Met expression in a panel of human gastric cell lines. mAb12.1-HRP recognises 190kD pro-s-Met and 50kD mature c-Met alpha chain. mAb 12.1-HRP does not recognise the 145kD mature c-Met beta-chain.")
![Immunohistochemistry: VEGFR1/Flt-1 Antibody (Z9P4F3/H10) [NBP2-50408] - Staining of H10 on whole-mount zebrafish embryo.](http://images.novusbio.com/fullsize/VEGF-R1-Flt-1-Antibody-Z9P4F3H10-Immunofluorescence-NBP2-50408-img0001.jpg "Immunohistochemistry: VEGFR1/Flt-1 Antibody (Z9P4F3/H10) [NBP2-50408] - Staining of H10 on whole-mount zebrafish embryo.")
![Western Blot: ZAP70 [p Tyr352] Antibody (GT364) [NBP3-13621] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT364] (NBP3-13621) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT364-Western-Blot-NBP3-13621-img0002.jpg "Western Blot: ZAP70 [p Tyr352] Antibody (GT364) [NBP3-13621] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT364] (NBP3-13621) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: ZAP70 [p Tyr352] Antibody (GT364) [NBP3-13621] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Zap70 (phospho Tyr319) + Syk (phospho Tyr352) antibody [GT364] (NBP3-13621) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT364-Western-Blot-NBP3-13621-img0001.jpg)
![Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0002.jpg "Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT1 [p Ser129] Antibody [NBP3-13418] - Untreated (-) and treated (+) Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Ser129) antibody (NBP3-13418) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT1-[p-Ser129]-Antibody-Western-Blot-NBP3-13418-img0001.jpg)
![Western Blot: Insulin R/CD220 Antibody [NBP1-74140] - Titration: 1.0 ug/ml Positive Control: Mouse Pancreas.](http://images.novusbio.com/fullsize/Insulin-R-CD220-Antibody-Western-Blot-NBP1-74140-img0003.jpg "Western Blot: Insulin R/CD220 Antibody [NBP1-74140] - Titration: 1.0 ug/ml Positive Control: Mouse Pancreas.")
![Immunoprecipitation: ZAP70 [p Tyr494] Antibody [NBP1-18898] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-ZAP70 antibody NB100-97826 was used at 3 mcg/mg lysate to immunoprecipitate ZAP70. The immunoprecipites were either treated with phosphatase (+) or mock (-) treated](http://images.novusbio.com/fullsize/ZAP70-[p-Tyr494]-Antibody-Immunoprecipitation-NBP1-18898-img0003.jpg "Immunoprecipitation: ZAP70 [p Tyr494] Antibody [NBP1-18898] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-ZAP70 antibody NB100-97826 was used at 3 mcg/mg lysate to immunoprecipitate ZAP70. The immunoprecipites were either treated with phosphatase (+) or mock (-) treated")
![Immunoprecipitation: ZAP70 [p Tyr494] Antibody [NBP1-18898] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Affinity purified rabbit anti-Phospho ZAP70 antibodies NBP1-18896 (for phosphorylation at Y319), NBP1-18897 (for phosphorylation at Y493), and NBP1-18898 (for phosphorylation at Y493/Y494) used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/ZAP70-[p-Tyr494]-Antibody-Immunoprecipitation-NBP1-18898-img0004.jpg)
![Flow Cytometry: TCR alpha Antibody (F1) [NBP2-22493] - Analysis of FITC conjugate of NBP2-22493. Flow cytometry TCR V alpha 2 (F1) on TCR V alpha 2 positive MOLT16 cells (left panel) or negative control HPB-ALL cells (right panel). Equal numbers of cells were stained with a FITC labeled TCR V alpha 2 (F1) mon](http://images.novusbio.com/fullsize/TCR-alpha-Antibody-F1-Flow-Cytometry-NBP2-22493-img0001.jpg "Flow Cytometry: TCR alpha Antibody (F1) [NBP2-22493] - Analysis of FITC conjugate of NBP2-22493. Flow cytometry TCR V alpha 2 (F1) on TCR V alpha 2 positive MOLT16 cells (left panel) or negative control HPB-ALL cells (right panel). Equal numbers of cells were stained with a FITC labeled TCR V alpha 2 (F1) mon")
![Immunoprecipitation: MEK1 Antibody [NBP3-14688] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-MEK1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. MEK1 was alsoimmunoprecipitated by rabbit anti-MEK1 recombinantmonoclonal antibody. For blottingimmunoprecipitated MEK1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MEK1-Antibody-Immunoprecipitation-NBP3-14688-img0001.jpg "Immunoprecipitation: MEK1 Antibody [NBP3-14688] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-MEK1recombinant monoclonal antibody used for IP at 20 ul/mg lysate. MEK1 was alsoimmunoprecipitated by rabbit anti-MEK1 recombinantmonoclonal antibody. For blottingimmunoprecipitated MEK1, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: YAP1 [p Ser128] Antibody [NBP3-16939] - Western blot analysis of extracts of NIH/3T3 cells, using YAP1 antibody (NBP3-16939) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/YAP1-[p-Ser128]-Antibody-Western-Blot-NBP3-16939-img0001.jpg "Western Blot: YAP1 [p Ser128] Antibody [NBP3-16939] - Western blot analysis of extracts of NIH/3T3 cells, using YAP1 antibody (NBP3-16939) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.")
![Western Blot: YAP1 [p Ser128] Antibody - Azide and BSA Free [YAP1] - Western blot analysis of lysates from HeLa cells using YAP1 Rabbit pAb at 1:2000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-16939_rabbit-yap1-p-ser-128-pab-52202517352786.jpg)