| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35336-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03913-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04538-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15528-20ul | |||
![Western Blot: Laminin beta 1 Antibody [NBP3-35336] - Western blot analysis of various lysates using Laminin beta 1 Rabbit pAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35336_rabbit-laminin-beta-1-pab-101220241233876.jpg "Western Blot: Laminin beta 1 Antibody [NBP3-35336] - Western blot analysis of various lysates using Laminin beta 1 Rabbit pAb at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: Laminin beta 1 Antibody [NBP3-35336] - Immunofluorescence analysis of L929 cells using Laminin beta 1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35336_rabbit-laminin-beta-1-pab-1012202411552810.jpg)
![Western Blot: LATS2 Antibody [NBP3-03913] - Analysis of extracts of various cell lines, using LATS2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/LATS2-Antibody-Western-Blot-NBP3-03913-img0004.jpg "Western Blot: LATS2 Antibody [NBP3-03913] - Analysis of extracts of various cell lines, using LATS2 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: ING5 Antibody [NBP3-04538] - Western blot analysis of extracts of 293T cells, using ING5 antibody (NBP3-04538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ING5 Antibody-Western Blot-NBP3-04538-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ING5 Antibody [NBP3-04538] - Western blot analysis of extracts of 293T cells, using ING5 antibody (NBP3-04538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ING5 Antibody [NBP3-04538] - Analysis of MCF-7 cells using ING5 antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ING5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-04538-img0001.jpg)
![Western Blot: TSC2 Antibody (7X2C6) [NBP3-15763] - Western blot analysis of extracts of various cell lines, using TSC2 antibody (NBP3-15763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/TSC2-Antibody-7X2C6-Western-Blot-NBP3-15763-img0001.jpg "Western Blot: TSC2 Antibody (7X2C6) [NBP3-15763] - Western blot analysis of extracts of various cell lines, using TSC2 antibody (NBP3-15763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunoprecipitation: TSC2 Antibody (7X2C6) [NBP3-15763] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug TSC2 antibody (NBP3-15763). Western blot was performed from the immunoprecipitate using TSC2 antibody (NBP3-15763) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/TSC2-Antibody-7X2C6-Immunoprecipitation-NBP3-15763-img0002.jpg)
![Western Blot: Bim Antibody (3R0T2) [NBP3-15867] - Western blot analysis of extracts of various cell lines, using Bim antibody (NBP3-15867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Bim-Antibody-3R0T2-Western-Blot-NBP3-15867-img0001.jpg "Western Blot: Bim Antibody (3R0T2) [NBP3-15867] - Western blot analysis of extracts of various cell lines, using Bim antibody (NBP3-15867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Bim Antibody (3R0T2) [NBP3-15867] - Western blot analysis of extracts of various cell lines, using Bim antibody (NBP3-15867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Bim-Antibody-3R0T2-Western-Blot-NBP3-15867-img0002.jpg)
![Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aurora-A-[p-Thr288]-Antibody-Western-Blot-NBP3-05434-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Aurora A [p Thr288] Antibody [NBP3-05434] - Western blot analysis of extracts of HeLa and HT-29 cells, using Aurora A antibody (NBP3-05434) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours. Hela cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.")
![<b>Biological Strategies Validation. </b>Simple Western: Aurora A [p Thr288] Antibody - BSA Free [NBP3-05434] - Analysis of protein expression post-drug treatment. (A) CHLA-10 and (B) TC-71 cells treated with drugs were assessed for changes in protein expression 24 h post-treatment via capillary electrophoresis-based Wes analysis. Increased protein levels of KIF11, p-KIF11Thr926 AURKA, and p-AURKAThr288 were observed for the drug combination group, whereas KIF15 levels were noticeably lower. Similarly, enhanced cleaved-PARP expression was observed with the combination treatment. The uncropped blots are shown in Figures S8 and S9. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/37894278 ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp3-05434_rabbit-polyclonal-aurora-a-p-thr288-antibody-308202410445511.jpg)
![SDS-Page: Recombinant Human MMP-2 Protein [NBP2-53037] - 15% SDS-PAGE (3ug)](http://images.novusbio.com/fullsize/MMP-2-Recombinant-Protein-SDS-Page-NBP2-53037-img0001.jpg "SDS-Page: Recombinant Human MMP-2 Protein [NBP2-53037] - 15% SDS-PAGE (3ug)")
![SDS-Page: Recombinant Human ERK2 Protein [NBP1-30309] - 15% SDS-Page (3ug)](http://images.novusbio.com/fullsize/Recombinant-Human-ERK2-Protein-SDS-Page-NBP1-30309-img0001.jpg "SDS-Page: Recombinant Human ERK2 Protein [NBP1-30309] - 15% SDS-Page (3ug)")
![Western Blot: KLF13 Antibody [NBP3-15528] - Western blot analysis of extracts of various cell lines, using KLF13 antibody (NBP3-15528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/KLF13-Antibody-Western-Blot-NBP3-15528-img0001.jpg "Western Blot: KLF13 Antibody [NBP3-15528] - Western blot analysis of extracts of various cell lines, using KLF13 antibody (NBP3-15528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot:DCC Antibody (OTI5D10)[NBP3-44889]](http://images.novusbio.com/fullsize/nbp3-44889_mouse-dcc-mab-oti5d10-western-blot-18120261617598.jpg "Western Blot:DCC Antibody (OTI5D10)[NBP3-44889]")
![Western Blot: Menin Antibody (5M7L9) [NBP3-16180] - Western blot analysis of extracts of various cell lines, using Menin Rabbit mAb (NBP3-16180) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Menin-Antibody-5M7L9-Western-Blot-NBP3-16180-img0001.jpg "Western Blot: Menin Antibody (5M7L9) [NBP3-16180] - Western blot analysis of extracts of various cell lines, using Menin Rabbit mAb (NBP3-16180) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Menin Antibody (5M7L9) [NBP3-16180] - Western blot analysis of extracts of various cell lines, using Menin Rabbit mAb (NBP3-16180) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Menin-Antibody-5M7L9-Western-Blot-NBP3-16180-img0002.jpg)