| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-82107SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38243-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37329-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37441-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52475-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51664SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22391SS | |||
| Azide and BSA Free | NBP2-80934 | |||
![Western Blot: MTA2 Antibody (9A5Y7) [NBP3-16291] - Western blot analysis of extracts of various cell lines, using MTA2 Rabbit mAb (NBP3-16291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/MTA2-Antibody-9A5Y7-Western-Blot-NBP3-16291-img0003.jpg "Western Blot: MTA2 Antibody (9A5Y7) [NBP3-16291] - Western blot analysis of extracts of various cell lines, using MTA2 Rabbit mAb (NBP3-16291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: MTA2 Antibody (9A5Y7) [NBP3-16291] - Immunohistochemistry of paraffin-embedded human colon using MTA2 Rabbit mAb (NBP3-16291) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MTA2-Antibody-9A5Y7-Immunohistochemistry-Paraffin-NBP3-16291-img0002.jpg)
![Chromatin Immunoprecipitation: MTA2 Antibody (9A5Y7) [NBP3-16291] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MTA2 antibody (NBP3-16291) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/MTA2-Antibody-9A5Y7-Chromatin-Immunoprecipitation-NBP3-16291-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MTA2 Antibody (9A5Y7) [NBP3-16291] - Western blot analysis of extracts of 293T cells, using MTA2 antibody (NBP3-16291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MTA2-Antibody-9A5Y7-Knockout-Validated-NBP3-16291-img0001.jpg)
![Chromatin Immunoprecipitation: MTA2 Antibody (9A5Y7) [NBP3-16291] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MTA2 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16291_rabbit-mta2-mab-9a5y7-5220251265321.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in HepG2 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 ug/mL Lane 2: 0.5 ug/mL Lane 3: 0.25 ug/mL with blocking peptide](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0007.jpg "Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in HepG2 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 ug/mL Lane 2: 0.5 ug/mL Lane 3: 0.25 ug/mL with blocking peptide")
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in Human, Mouse, and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 [NBP2-82107], (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 NBP2-82107 (1 ug/mL), JMJD3 from Company B, (2 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0003.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in HeLa Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107 (0.25 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0004.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in K562 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107 (0.5 ug/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: in the absence and Lane 2: in the presence of blocking peptide.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0005.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in Mouse A-20 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107 (0.5 ug/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: in the absence and Lane 2: in the presence of blocking peptide](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Immunocytochemistry Validation of JMJD3 in K562 Cells. Immunocytochemical analysis of K562 cells using Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 antibody (NBP2-82107) at 2.5 ug/ml. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82107-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP2-82107] - Validation of Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp2-82107_rabbit-lysine-k-specific-demethylase-6b-kdm6b-jmjd3-pab-271120241937784.jpg)
![Immunocytochemistry/ Immunofluorescence: GAS41 Antibody [NBP3-38243] - Immunofluorescence analysis of A-549 cells using GAS41 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38243_rabbit-gas41-pab-101220241233892.jpg "Immunocytochemistry/ Immunofluorescence: GAS41 Antibody [NBP3-38243] - Immunofluorescence analysis of A-549 cells using GAS41 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: GAS41 Antibody [NBP3-38243] - Immunohistochemistry analysis of paraffin-embedded Rat lung using GAS41 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38243_rabbit-gas41-pab-101220241224184.jpg)
![Western Blot: GAS41 Antibody [NBP3-38243] - Western blot analysis of lysates from 293T cells, using GAS41 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-38243_rabbit-gas41-pab-1012202411592883.jpg)
![Immunohistochemistry: GAS41 Antibody [NBP3-38243] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using GAS41 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38243_rabbit-gas41-pab-1012202411531076.jpg)
![Immunocytochemistry/ Immunofluorescence: GAS41 Antibody [NBP3-38243] - Immunofluorescence analysis of HeLa cells using GAS41 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38243_rabbit-gas41-pab-1012202411552825.jpg)
![Immunohistochemistry: GAS41 Antibody [NBP3-38243] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using GAS41 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38243_rabbit-gas41-pab-1012202412063.jpg)
![Immunocytochemistry/ Immunofluorescence: GAS41 Antibody [NBP3-38243] - Immunofluorescence analysis of U2OS cells using GAS41 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38243_rabbit-gas41-pab-1012202411545525.jpg)
![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0003.jpg "Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against HEK293 (1) and ZEB1 (AA: 967-1108)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunofluorescence analysis of Hela cells using ZEB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunocytochemistry-Immunofluorescence-NBP2-37329-img0004.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0007.jpg)
![Flow Cytometry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Flow-Cytometry-NBP2-37329-img0005.jpg)
![Western Blot: ZEB1 Antibody (2A8H3) [NBP2-37329] - Western blot analysis using ZEB1 mAb against human ZEB1 recombinant protein. (Expected MW is 41.7 kDa)](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Western-Blot-NBP2-37329-img0002.jpg)
![Immunohistochemistry: ZEB1 Antibody (2A8H3) [NBP2-37329] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-Immunohistochemistry-NBP2-37329-img0006.jpg)
![ELISA: ZEB1 Antibody (2A8H3) [NBP2-37329] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8H3-ELISA-NBP2-37329-img0001.jpg)
![Western Blot: STAT5a Antibody (6D4) [NBP2-37441] - Western blot analysis using STAT5A mouse mAb against K562 (1), MOLT4 (2), HeLa (3), Jurkat (4), and A431 (5) cell lysate.](http://images.novusbio.com/fullsize/STAT5a-Antibody-6D4-Western-Blot-NBP2-37441-img0003.jpg "Western Blot: STAT5a Antibody (6D4) [NBP2-37441] - Western blot analysis using STAT5A mouse mAb against K562 (1), MOLT4 (2), HeLa (3), Jurkat (4), and A431 (5) cell lysate.")
![Immunocytochemistry/Immunofluorescence: STAT5a Antibody (6D4) [NBP2-37441] - Immunofluorescence analysis of Hela cells using STAT5A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/STAT5a-Antibody-6D4-Immunocytochemistry-Immunofluorescence-NBP2-37441-img0004.jpg)
![Immunohistochemistry: STAT5a Antibody (6D4) [NBP2-37441] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using STAT5A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/STAT5a-Antibody-6D4-Immunohistochemistry-NBP2-37441-img0007.jpg)
![Flow Cytometry: STAT5a Antibody (6D4) [NBP2-37441] - Flow cytometric analysis of K562 cells using STAT5A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/STAT5a-Antibody-6D4-Flow-Cytometry-NBP2-37441-img0005.jpg)
![Western Blot: STAT5a Antibody (6D4) [NBP2-37441] - Western blot analysis using STAT5A mAb against human STAT5A recombinant protein. (Expected MW is 49.3 kDa)](http://images.novusbio.com/fullsize/STAT5a-Antibody-6D4-Western-Blot-NBP2-37441-img0002.jpg)
![Immunohistochemistry: STAT5a Antibody (6D4) [NBP2-37441] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using STAT5A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/STAT5a-Antibody-6D4-Immunohistochemistry-NBP2-37441-img0006.jpg)
![ELISA: STAT5a Antibody (6D4) [NBP2-37441] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/STAT5a-Antibody-6D4-ELISA-NBP2-37441-img0001.jpg)
![Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against human GLI1 (AA: 284-449) recombinant protein. (Expected MW is 43.1 kDa)](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Western-Blot-NBP2-52475-img0005.jpg "Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against human GLI1 (AA: 284-449) recombinant protein. (Expected MW is 43.1 kDa)")
![Immunocytochemistry/Immunofluorescence: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of Hela cells using GLI1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Immunofluorescence-NBP2-52475-img0002.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of esophageal cancer tissues using GLI1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Immunohistochemistry-NBP2-52475-img0004.jpg)
![Flow Cytometry: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of HepG2 cells using GLI1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Flow-Cytometry-NBP2-52475-img0001.jpg)
![Western Blot: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis using GLI1 mAb against HEK293 (1) and GLI1 (AA: 284-449)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Western-Blot-NBP2-52475-img0006.jpg)
![Immunohistochemistry-Paraffin: GLI-1 Antibody (1B9F8) [NBP2-52475] - Analysis of ovarian cancer tissues using GLI1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-Immunohistochemistry-NBP2-52475-img0003.jpg)
![ELISA: GLI-1 Antibody (1B9F8) [NBP2-52475] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/GLI-1-Antibody-1B9F8-ELISA-NBP2-52475-img0008.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOS .](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Western-Blot-NBP2-70764-img0002.jpg "Western Blot: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOS .")
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0007.jpg)
![Western Blot: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of extracts (10ug) from 4 different cell lines by using anti-FOS monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Western-Blot-NBP2-70764-img0001.jpg)
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0003.jpg)
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0004.jpg)
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0005.jpg)
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0006.jpg)
![Immunohistochemistry: c-Fos Antibody (OTI7D6) - Azide and BSA Free [NBP2-70764] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/c-Fos-Antibody-OTI7D6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70764-img0008.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Western-Blot-NBP2-70687-img0001.jpg "Western Blot: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Estrogen Receptor alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant")
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0008.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0002.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0003.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human breast tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0004.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human Kidney tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0005.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human lymphoma tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0006.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human pancreas tissue using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0007.jpg)
![Immunohistochemistry: ER alpha/NR3A1 Antibody (OTI1B1) - Azide and BSA Free [NBP2-70687] - Staining of paraffin-embedded Human tonsil using anti-Estrogen Receptor alpha mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-OTI1B1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70687-img0009.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NFKB2 antibody NBP1-87759 (A) shows similar protein distribution across tissues to independent antibody NBP1-87760 (B).](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: NFkB p100/p52 Antibody [NBP1-87759] - Western blot analysis in human cell lines A-431 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Western-Blot-NBP1-87759-img0009.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human kidney.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0014.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0006.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0007.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human lymph node.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0012.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human testis.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0013.jpg)
![Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human cerebral cortex using Anti-NFKB2 antibody.](http://images.novusbio.com/fullsize/NFkB-p100-p52-Antibody-Immunohistochemistry-Paraffin-NBP1-87759-img0015.jpg)
![Western Blot: OCT4 Antibody (7E7) [NBP1-51664] - Analysis using OCT4 mouse mAb against NTERA-2 cell lysate.](http://images.novusbio.com/fullsize/OCT4-Antibody-7E7-Western-Blot-NBP1-51664-img0007.jpg "Western Blot: OCT4 Antibody (7E7) [NBP1-51664] - Analysis using OCT4 mouse mAb against NTERA-2 cell lysate.")
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody (7E7) [NBP1-51664] - Analysis of NTERA-2 cells using OCT4 mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).](http://images.novusbio.com/fullsize/OCT4-Antibody-7E7-Immunocytochemistry-Immunofluorescence-NBP1-51664-img0008.jpg)
![Flow Cytometry: OCT4 Antibody (7E7) [NBP1-51664] - Flow cytometric analysis of Jurket cells using OCT4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/OCT4-Antibody-7E7-Flow-Cytometry-NBP1-51664-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: OCT4 Antibody (7E7) [NBP1-51664] - Analysis of NTERA-2 cells using OCT4 mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).](http://images.novusbio.com/fullsize/OCT4-Antibody-7E7-Immunocytochemistry-Immunofluorescence-NBP1-51664-img0006.jpg)
![Western Blot: RUNX3/CBFA3 Antibody (2B10E8) [NBP2-22391] - Analysis of a partial recombinant Runx3 protein probed with Runx3 antibody at 0.5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/RUNX3-CBFA3-Antibody-2B10E8-Western-Blot-NBP2-22391-img0008.jpg "Western Blot: RUNX3/CBFA3 Antibody (2B10E8) [NBP2-22391] - Analysis of a partial recombinant Runx3 protein probed with Runx3 antibody at 0.5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody (2B10E8) [NBP2-22391] - Staining of normal human spleen tissue stained with Runx3 antibody (3 ug/ml), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/RUNX3-CBFA3-Antibody-2B10E8-Immunohistochemistry-Paraffin-NBP2-22391-img0007.jpg)
![Flow Cytometry: RUNX3/CBFA3 Antibody (2B10E8) [NBP2-22391] - Intracellular flow analysis of human lymphocytes probed with Runx3 antibody (red) and isotype control (green) at 0.25 ug/10^6 cells.](http://images.novusbio.com/fullsize/RUNX3-CBFA3-Antibody-2B10E8-Flow-Cytometry-NBP2-22391-img0006.jpg)