| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89196-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44080 | |||
| BSA Free | NBP2-61411-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35268-1mg | |||
| Carrier-Free | NBP2-35268-100ug | |||
| Carrier-Free | NBP2-35268-500ug | |||
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0002.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human testis with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0001.jpg)
![Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-26820241525104.jpg "Chromatin Immunoprecipitation: SOX2 Antibody (PO00-28) [NBP3-32972] - Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either SOX2 (NBP3-32972) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.")
![Western Blot: SOX2 Antibody (PO00-28) [NBP3-32972] - Western blot analysis of SOX2 on different lysates with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lane 3: HeLa cell lysate (negative) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32972) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-28820241249344.jpg)
![Immunohistochemistry: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-298202414161844.jpg)
![Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32972_rabbit-sox2-mab-po00-28-29202410334048.jpg)
![SDS-Page: Recombinant Human Pax6 Protein [H00005080-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005080-P01-1.jpg "SDS-Page: Recombinant Human Pax6 Protein [H00005080-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunocytochemistry/Immunofluorescence: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of HEK 293 cells using the Anti-PAX6 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pax6-Antibody-CL5414-Immunocytochemistry-Immunofluorescence-NBP2-61411-img0001.jpg "Immunocytochemistry/Immunofluorescence: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of HEK 293 cells using the Anti-PAX6 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of rat eye retina shows strong nuclear positivity in the inner nuclear layer and ganglion cell layer.](http://images.novusbio.com/fullsize/Pax6-Antibody-CL5414-Immunohistochemistry-Paraffin-NBP2-61411-img0007.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of mouse embryo E11 shows strong nuclear positivity in the developing brain and sensory organs.](http://images.novusbio.com/fullsize/Pax6-Antibody-CL5414-Immunohistochemistry-Paraffin-NBP2-61411-img0005.jpg)
![Immunohistochemistry-Paraffin: Pax6 Antibody (CL5414) [NBP2-61411] - Staining of mouse embryo E14 shows strong nuclear positivity in the developing eye.](http://images.novusbio.com/fullsize/Pax6-Antibody-CL5414-Immunohistochemistry-Paraffin-NBP2-61411-img0006.jpg)
![SDS-Page: Human SOX2 TAT Protein [NBP2-35268]](http://images.novusbio.com/fullsize/Human-SOX2-TAT-Protein-SDS-Page-NBP2-35268-img0001.jpg "SDS-Page: Human SOX2 TAT Protein [NBP2-35268]")
![Western Blot: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Western blot analysis of extracts of various cell lines, using PAWR / PAR4 Rabbit mAb (NBP3-16523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-10Q2G9-Western-Blot-NBP3-16523-img0003.jpg "Western Blot: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Western blot analysis of extracts of various cell lines, using PAWR / PAR4 Rabbit mAb (NBP3-16523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Immunofluorescence analysis of HeLa cells using PAWR / PAR4 Rabbit mAb (NBP3-16523) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-10Q2G9-Immunocytochemistry-Immunofluorescence-NBP3-16523-img0002.jpg)
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Immunohistochemistry of paraffin-embedded human colon carcinoma using PAWR / PAR4 Rabbit mAb (NBP3-16523) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-10Q2G9-Immunohistochemistry-Paraffin-NBP3-16523-img0001.jpg)
![Western Blot: Pax6 Antibody [NB100-61654] - (0.5ug/ml) staining of RAW264.7 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB100-61654-img0002.jpg "Western Blot: Pax6 Antibody [NB100-61654] - (0.5ug/ml) staining of RAW264.7 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Pax6 Antibody [NB100-61654] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Flow-Cytometry-NB100-61654-img0003.jpg)
![Western Blot: Pax6 Antibody [NB100-61654] - (0.3ug/ml) staining of U251 (A), (0.03ug/ml) nuclear K562 (B) and (1ug/ml) nuclear HeLa (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB100-61654-img0001.jpg)
![Western Blot: Pax6 Antibody (GT9412) [NBP3-13636] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/Pax6-Antibody-GT9412-Western-Blot-NBP3-13636-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Pax6 Antibody (GT9412) [NBP3-13636] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Pax6-Antibody-GT9412-Immunohistochemistry-Paraffin-NBP3-13636-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Pax6 Antibody (GT9412) [NBP3-13636] - Pax6 antibody [GT9412] detects Pax6 protein at nucleus by immunofluorescent analysis.Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pax6 stained by Pax6 antibody [GT9412] (NBP3-13636) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-13636_mouse-pax6-mab-gt9412-91020242011450.jpg)
![Immunofluorescence:SOX2 Antibody[NBP3-31961]- staining of NTERA2 cells stained with Novus Biologicals anti-SOX2 antibody (green) showing strong nuclear staining of endogenous SOX2. Actin filaments are stained with phalloidin (red).](http://images.novusbio.com/fullsize/antibody/nbp3-31961_chicken-sox2-pab-immunofluorescence-1911202413196..jpg "Immunofluorescence:SOX2 Antibody[NBP3-31961]- staining of NTERA2 cells stained with Novus Biologicals anti-SOX2 antibody (green) showing strong nuclear staining of endogenous SOX2. Actin filaments are stained with phalloidin (red).")
![Western Blot:SOX2 Antibody[NBP3-31961]- Western blotting of SOX2-FLAG transfected COS-7 cell lysate (10 µg/lane), mock transfected COS-7 cell lysate (10 µg/lane) and NTERA2 cell lysate (10 µg/lane) and stained with Novus Biologicals anti-SOX2 antibody (1µg/mL).Note that SOX2 runs at higher molecular weight in lane 1 due to presence of tandem Myc/FLAG tag on recombinant protein relative to endogenous SOX2 in lane 3.](http://images.novusbio.com/fullsize/antibody/nbp3-31961_chicken-sox2-pab-western-blot-19112024132246..jpg)
![Western Blot: SOX2 Antibody [NBP2-58318] - Analysis in human cell line U-251 MG and human cell line HeLa.](http://images.novusbio.com/fullsize/SOX2-Antibody-Western-Blot-NBP2-58318-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SOX2 Antibody [NBP2-58318] - Analysis in human cell line U-251 MG and human cell line HeLa.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NBP2-58318] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SOX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58318-img0001.jpg)
![Western Blot: PAWR / PAR4 Antibody (3G9H7) [NBP1-28892] - PAWR / PAR4 Antibody (3G9H7) Western blot analysis using PAR4 mouse mAb against full-length Trx-Par4 recombinant protein (1) and Hela cell lysate (2).](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-3G9H7-Western-Blot-NBP1-28892-img0006.jpg "Western Blot: PAWR / PAR4 Antibody (3G9H7) [NBP1-28892] - PAWR / PAR4 Antibody (3G9H7) Western blot analysis using PAR4 mouse mAb against full-length Trx-Par4 recombinant protein (1) and Hela cell lysate (2).")
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody (3G9H7) [NBP1-28892] - Analysis of human brain (left) and breast carcinoma (right), showing cytoplasmic and membrane localization using PAR4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-3G9H7-Immunohistochemistry-Paraffin-NBP1-28892-img0005.jpg)
![Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody (3G9H7) [NBP1-28892] - Analysis of human Small Intestine tissues using PAR4 mouse mAb.](http://images.novusbio.com/fullsize/PAWR-PAR4-Antibody-3G9H7-Immunohistochemistry-Paraffin-NBP1-28892-img0004.jpg)