| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44121 | |||
| BSA Free | NBP2-59033-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79911 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32381-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61768-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-07075SS | |||
![Western Blot: HEY1 Antibody (3B3) [H00023462-M01] - analysis of HEY1 expression in transfected 293T cell line by HEY1 monoclonal antibody (M01), clone 3B3. Lane 1: HEY1 transfected lysate (32.613 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HEY1-Antibody-3B3-Western-Blot-H00023462-M01-img0001.jpg "Western Blot: HEY1 Antibody (3B3) [H00023462-M01] - analysis of HEY1 expression in transfected 293T cell line by HEY1 monoclonal antibody (M01), clone 3B3. Lane 1: HEY1 transfected lysate (32.613 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HEY1 Antibody (3B3) [H00023462-M01] - Analysis of monoclonal antibody to HEY1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/HEY1-Antibody-3B3-Immunocytochemistry-Immunofluorescence-H00023462-M01-img0004.jpg)
![ELISA: HEY1 Antibody (3B3) [H00023462-M01] - Detection limit for recombinant GST tagged HEY1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HEY1-Antibody-3B3-Sandwich-ELISA-H00023462-M01-img0005.jpg)
![Western Blot: FRA2 Antibody (2B4-1C2) [H00002355-M01] - FOSL2 monoclonal antibody (M01), clone 2B4-1C2 Analysis of FOSL2 expression in MCF-7.](http://images.novusbio.com/fullsize/FRA2-Antibody-2B4-1C2-Western-Blot-H00002355-M01-img0010.jpg "Western Blot: FRA2 Antibody (2B4-1C2) [H00002355-M01] - FOSL2 monoclonal antibody (M01), clone 2B4-1C2 Analysis of FOSL2 expression in MCF-7.")
![Immunocytochemistry/Immunofluorescence: FRA2 Antibody (2B4-1C2) [H00002355-M01] - Analysis of monoclonal antibody to FOSL2 on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/FRA2-Antibody-2B4-1C2-Immunocytochemistry-Immunofluorescence-H00002355-M01-img0007.jpg)
![Western Blot: FRA2 Antibody (2B4-1C2) [H00002355-M01] - Western Blot analysis of FOSL2 expression in Jurkat ( Cat # L017V1 ).](http://images.novusbio.com/fullsize/FRA2-Antibody-2B4-1C2-Western-Blot-H00002355-M01-img0001.jpg)
![Western Blot: FRA2 Antibody (2B4-1C2) [H00002355-M01] - FOSL2 monoclonal antibody (M01), clone 2B4-1C2. Analysis of FOSL2 expression in Jurkat.](http://images.novusbio.com/fullsize/FRA2-Antibody-2B4-1C2-Western-Blot-H00002355-M01-img0009.jpg)
![Sandwich ELISA: FRA2 Antibody (2B4-1C2) [H00002355-M01] - Detection limit for recombinant GST tagged FOSL2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FRA2-Antibody-2B4-1C2-Sandwich-ELISA-H00002355-M01-img0008.jpg)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-278202415212459.jpg "Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32527) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-298202414333615.jpg)
![Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunocytochemistry analysis of Jurkat (positive) and HeLa (negative) labeling LEF1 with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-2920241023422.jpg)
![Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody [NBP2-59033] -Analysis in human fallopian tube and skeletal muscle tissues using NBP2-59033 antibody. Corresponding FOXJ1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-59033_mouse-monoclonal-foxj1-hfh4-antibody-cl3991-immunohistochemistry-paraffin-26220258433..png "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody [NBP2-59033] -Analysis in human fallopian tube and skeletal muscle tissues using NBP2-59033 antibody. Corresponding FOXJ1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody [NBP2-59033] - Staining of human skeletal muscle shows no positivity as expected (negative control).](http://images.novusbio.com/fullsize/FoxJ1-HFH4-Antibody-Immunohistochemistry-Paraffin-NBP2-59033-img0003.jpg)
![Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody [NBP2-59033] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FoxJ1-HFH4-Antibody-Immunohistochemistry-Paraffin-NBP2-59033-img0001.jpg)
![Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody [NBP2-59033] - Staining of human endometrium shows strong nuclear immunoreactivity in some glandular cells.](http://images.novusbio.com/fullsize/FoxJ1-HFH4-Antibody-Immunohistochemistry-Paraffin-NBP2-59033-img0002.jpg)
![Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody [NBP2-59033] -Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-59033_mouse-monoclonal-foxj1-hfh4-antibody-cl3991-immunohistochemistry-paraffin-26220258420..png)
![Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Small Intestine stained with HNF1 Antibody (HNF1A/2087).](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Immunohistochemistry-Paraffin-NBP2-79777-img0003.jpg "Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Small Intestine stained with HNF1 Antibody (HNF1A/2087).")
![Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Immunohistochemistry-Paraffin-NBP2-79777-img0001.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Immunohistochemistry-Paraffin-NBP2-79777-img0002.jpg)
![Protein Array: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HNF1 Antibody (HNF1A/2087). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Protein-Array-NBP2-79777-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Pax3 Antibody [NBP2-32381] - Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pax3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32381-img0014.jpg "Immunocytochemistry/Immunofluorescence: Pax3 Antibody [NBP2-32381] - Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Pax3 Antibody [NBP2-32381] - Staining of human testis shows moderate positivity in nucleoli in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Pax3-Antibody-Immunohistochemistry-Paraffin-NBP2-32381-img0018.jpg)
![Immunohistochemistry-Paraffin: Pax3 Antibody [NBP2-32381] - Staining of human prostate shows no positivity in nucleoli in glandular cells as expected.](http://images.novusbio.com/fullsize/Pax3-Antibody-Immunohistochemistry-Paraffin-NBP2-32381-img0016.jpg)
![Immunohistochemistry-Paraffin: Pax3 Antibody [NBP2-32381] - Staining of human skin shows strong positivity in nucleoli in squamous epithelial cells.](http://images.novusbio.com/fullsize/Pax3-Antibody-Immunohistochemistry-Paraffin-NBP2-32381-img0017.jpg)
![Western Blot: Pax3 Antibody (HL160) [NBP3-13661] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pax3 antibody [HL160] (NBP3-13661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax3-Antibody-HL160-Western-Blot-NBP3-13661-img0003.jpg "Western Blot: Pax3 Antibody (HL160) [NBP3-13661] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Pax3 antibody [HL160] (NBP3-13661) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Pax3 Antibody (HL160) [NBP3-13661] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Pax3 antibody [HL160] (NBP3-13661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Pax3-Antibody-HL160-Western-Blot-NBP3-13661-img0001.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Western Blot: Pax3 Antibody (HL160) [NBP3-13661] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Pax3 antibody [HL160] (NBP3-13661) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13661_rabbit-pax3-mab-hl160-910202420122099.jpg)
![Immunohistochemistry-Paraffin: Pax3 Antibody (HL160) [NBP3-13661] - Pax3 antibody [HL160] detects Pax3 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: Pax3 stained by Pax3 antibody [HL160] (NBP3-13661) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13661_rabbit-pax3-mab-hl160-910202420104438.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0004.jpg "Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against HEK293 (1) and ESRRA (AA: 198-376)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mAb against human ESRRA (AA: 198-376) recombinant protein. (Expected MW is 45.3 kDa)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0003.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis using ESRRA mouse mAb against C6 (1) cell lysate.](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-Western-Blot-NBP2-61768-img0005.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-ELISA-NBP2-61768-img0001.jpg)
![ELISA: ERR alpha/NR3B1 Antibody (6F1E6) [NBP2-61768] - Analysis of Hela cells using ESRRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ERR-alpha-NR3B1-Antibody-6F1E6-ELISA-NBP2-61768-img0002.jpg)
![Western Blot: Pax6 Antibody [NB100-61654] - (0.5ug/ml) staining of RAW264.7 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB100-61654-img0002.jpg "Western Blot: Pax6 Antibody [NB100-61654] - (0.5ug/ml) staining of RAW264.7 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Pax6 Antibody [NB100-61654] - Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Pax6-Antibody-Flow-Cytometry-NB100-61654-img0003.jpg)
![Western Blot: Pax6 Antibody [NB100-61654] - (0.3ug/ml) staining of U251 (A), (0.03ug/ml) nuclear K562 (B) and (1ug/ml) nuclear HeLa (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Pax6-Antibody-Western-Blot-NB100-61654-img0001.jpg)
![Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (2597C) [NBP3-07075] - HIF-2 alpha /EPAS1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Human HIF-2 alpha /EPAS1 Monoclonal Antibody (Catalog # NBP3-07075) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.](http://images.novusbio.com/fullsize/HIF-2-alpha-EPAS1-Antibody-2597C-Immunohistochemistry-Paraffin-NBP3-07075-img0001.jpg "Immunohistochemistry-Paraffin: HIF-2 alpha/EPAS1 Antibody (2597C) [NBP3-07075] - HIF-2 alpha /EPAS1 was detected in immersion fixed paraffin-embedded sections of mouse liver using Rabbit Anti-Human HIF-2 alpha /EPAS1 Monoclonal Antibody (Catalog # NBP3-07075) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.")
![Immunohistochemistry-Frozen: HEY1 Antibody [NBP2-16818] - Hey1 staining on E11.5 mouse Pharyngeal Mesoderm. Fixed 4% PFA overnight. Blocked with 1% BSA. Primary antibody dilution at 1:100. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HEY1-Antibody-Immunohistochemistry-Frozen-NBP2-16818-img0005.jpg "Immunohistochemistry-Frozen: HEY1 Antibody [NBP2-16818] - Hey1 staining on E11.5 mouse Pharyngeal Mesoderm. Fixed 4% PFA overnight. Blocked with 1% BSA. Primary antibody dilution at 1:100. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a verified customer review.")
![HEY1 antibody detects HEY1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HEY1 protein stained by HEY1 antibody (NBP2-16818) diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16818_rabbit-polyclonal-hey1-antibody-immunocytochemistry-immunofluorescence-261120251838400.jpg)