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![Western Blot: Nucleoporin 107 Antibody (8E6H4) [NBP3-33250] - Western blot analysis of various lysates using Nucleoporin 107 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33250_rabbit-nucleoporin-107-mab-8e6h4-1012202411531034.jpg "Western Blot: Nucleoporin 107 Antibody (8E6H4) [NBP3-33250] - Western blot analysis of various lysates using Nucleoporin 107 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Western Blot: Nucleoporin 107 Antibody (8E6H4) [NBP3-33250] - Western blot analysis of lysates from Rat brain, using Nucleoporin 107 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33250_rabbit-nucleoporin-107-mab-8e6h4-101220241233856.jpg)
![Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] - Western blot analysis of various lysates using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33535_rabbit-ras-gap-mab-6w10g6-10122024140418.jpg "Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] - Western blot analysis of various lysates using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: Ras-GAP Antibody (6W10G6) [NBP3-33535] - Western blot analysis of lysates from mouse brain, using at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33535_rabbit-ras-gap-mab-6w10g6-10122024140449.jpg)
![Western Blot: DNA Primase large subunit Antibody [NBP2-92044] - Analysis of extracts of mouse spleen, using DNA Primase large subunit at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/DNA-Primase-large-subunit-Antibody-Western-Blot-NBP2-92044-img0001.jpg "Western Blot: DNA Primase large subunit Antibody [NBP2-92044] - Analysis of extracts of mouse spleen, using DNA Primase large subunit at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Western Blot: DNA Primase large subunit Antibody [NBP2-92044] - Analysis of extracts of various cell lines, using DNA Primase large subunit at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.](http://images.novusbio.com/fullsize/DNA-Primase-large-subunit-Antibody-Western-Blot-NBP2-92044-img0002.jpg)
![Western Blot: Numb Antibody (9X1Y0) [NBP3-16738] - Western blot analysis of extracts of various cell lines, using Numb antibody (NBP3-16738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Numb-Antibody-9X1Y0-Western-Blot-NBP3-16738-img0001.jpg "Western Blot: Numb Antibody (9X1Y0) [NBP3-16738] - Western blot analysis of extracts of various cell lines, using Numb antibody (NBP3-16738) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunoprecipitation: Numb Antibody (9X1Y0) [NBP3-16738] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Numb antibody (NBP3-16738). Western blot was performed from the immunoprecipitate using Numb (NBP3-16738) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Numb-Antibody-9X1Y0-Immunoprecipitation-NBP3-16738-img0002.jpg)
![Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (2B12) [NBP1-47444] - Analysis using AAT mouse mAb against human plasma (1) and NIH/3T3 cell lysate (2).](http://images.novusbio.com/fullsize/Serpin-A1-alpha-1-Antitrypsin-Antibody-2B12-Western-Blot-NBP1-47444-img0009.jpg "Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody (2B12) [NBP1-47444] - Analysis using AAT mouse mAb against human plasma (1) and NIH/3T3 cell lysate (2).")
![Western Blot: CDT2 Antibody (JE58-01) [NBP3-32176] - Western blot analysis of CDT2 on different lysates with Rabbit anti-CDT2 antibody (NBP3-32176) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32176) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32176_rabbit-cdt2-mab-je58-01-278202415295521.jpg "Western Blot: CDT2 Antibody (JE58-01) [NBP3-32176] - Western blot analysis of CDT2 on different lysates with Rabbit anti-CDT2 antibody (NBP3-32176) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: Mouse spleen tissue lysate (40 ug/Lane) Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32176) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: CDT2 Antibody (JE58-01) [NBP3-32176] - Flow cytometric analysis of HeLa cells labeling CDT2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32176, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32176_rabbit-cdt2-mab-je58-01-29202416372117.jpg)
![Western Blot: Protein Phosphatase 1C gamma Antibody [NBP3-30770] - Western blot using PP 1 gamma 2 antibody (Cat. No. NBP3-30770) on rat testes lysate.](http://images.novusbio.com/fullsize/nbp3-30770_sheep-protein-phosphatase-1c-gamma-pab-811202420592139.jpg "Western Blot: Protein Phosphatase 1C gamma Antibody [NBP3-30770] - Western blot using PP 1 gamma 2 antibody (Cat. No. NBP3-30770) on rat testes lysate.")
![Western Blot: PP2C beta/PPM1B Antibody [NBP3-30782] - Legend: Novus Biologicals' rabbit anti PP 2 A/B alpha/beta western blotting of total rat brain extract. Blocked with specific peptide (+). Note: Novus Biologicals' rabbit anti PP 2 A/B alpha/beta also readily detects recombinant PP 2 C alpha and PP 2 C beta.](http://images.novusbio.com/fullsize/nbp3-30782_rabbit-pp2c-beta-ppm1b-pab-81120242059215.jpg "Western Blot: PP2C beta/PPM1B Antibody [NBP3-30782] - Legend: Novus Biologicals' rabbit anti PP 2 A/B alpha/beta western blotting of total rat brain extract. Blocked with specific peptide (+). Note: Novus Biologicals' rabbit anti PP 2 A/B alpha/beta also readily detects recombinant PP 2 C alpha and PP 2 C beta.")
![Immunohistochemistry: CD20 Antibody (PD00-02) [NBP3-32131] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD20 antibody (NBP3-32131) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32131) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32131_rabbit-cd20-mab-pd00-02-29820241435213.jpg "Immunohistochemistry: CD20 Antibody (PD00-02) [NBP3-32131] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD20 antibody (NBP3-32131) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32131) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Protein Phosphatase 1C gamma Antibody [NBP3-30768] - Legend: Novus Biologicals' rabbit anti PP 1 gamma 1 antibody staining total rat brain homogenate.(+) indicates presence of blocking peptide or (-) control peptide. The darkest mark is located at -37 kDa.](http://images.novusbio.com/fullsize/nbp3-30768_rabbit-protein-phosphatase-1c-gamma-pab-811202420592137.jpg "Western Blot: Protein Phosphatase 1C gamma Antibody [NBP3-30768] - Legend: Novus Biologicals' rabbit anti PP 1 gamma 1 antibody staining total rat brain homogenate.(+) indicates presence of blocking peptide or (-) control peptide. The darkest mark is located at -37 kDa.")