| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27181-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31365SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21666-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11681-50ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89354-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86791-25ul | |||
![Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-278202415295570.jpg "Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29820241426267.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunocytochemistry analysis of HeLa cells labeling Lamin A + C with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29202410484838.jpg)
![Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis of Hela cells using Hyaluronan synthase 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27181_mouse-hyaluronan-synthase-1-mab-5b5b4-bsa-free-1042024917038.jpg%3Fv=1666704794 "Immunocytochemistry/Immunofluorescence: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis of Hela cells using Hyaluronan synthase 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Western Blot: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis using Hyaluronan synthase 1 mAb against human Hyaluronan synthase 1 (AA: 74-399) recombinant protein. (Expected MW is 40.2 kDa)](http://images.novusbio.com/fullsize/nbp3-27181_mouse-hyaluronan-synthase-1-mab-5b5b4-bsa-free-1042024917022.jpg%3Fv=1666704794)
![Flow Cytometry: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis of SK-OV-3 cells using Hyaluronan synthase 1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27181_mouse-hyaluronan-synthase-1-mab-5b5b4-bsa-free-10420249141923.jpg%3Fv=1666704794)
![ELISA: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27181_mouse-hyaluronan-synthase-1-mab-5b5b4-bsa-free-10420249181917.jpg%3Fv=1666704794)
![Western Blot: Hyaluronan synthase 1 Antibody (5B5B4) [NBP3-27181] - Analysis using Hyaluronan synthase 1 mAb against HEK293 (1) and Hyaluronan synthase 1 (AA:74-399)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nbp3-27181_mouse-hyaluronan-synthase-1-mab-5b5b4-bsa-free-1042024920246.jpg%3Fv=1666704794)
![Western Blot: OFD1 Antibody [NBP1-32843] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with OFD1 antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/OFD1-Antibody-Western-Blot-NBP1-32843-img0004.jpg "Western Blot: OFD1 Antibody [NBP1-32843] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with OFD1 antibody diluted at 1:5000.")
![Immunohistochemistry-Paraffin: OFD1 Antibody [NBP1-32843] - Paraffin-embedded gastric cancer. OFD1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/OFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-32843-img0002.jpg)
![Western Blot: OFD1 Antibody [NBP1-32843] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OFD1 antibody (NBP1-32843) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32843_rabbit-polyclonal-ofd1-antibody-91020242041257.jpg)
![Western Blot: 4-1BB/TNFRSF9/CD137 Antibody [NBP2-31365] - Detection of CD137 partial recombinant protein by using CD137 antibody at a concentration of 0.01 ug/ml.](http://images.novusbio.com/fullsize/CD137-Antibody-Western-Blot-NBP2-31365-img0001.jpg "Western Blot: 4-1BB/TNFRSF9/CD137 Antibody [NBP2-31365] - Detection of CD137 partial recombinant protein by using CD137 antibody at a concentration of 0.01 ug/ml.")
![Immunohistochemistry: RUNX1/2/3 Antibody (SR1906) [NBP3-21666] - Immunohistochemical analysis of paraffin-embedded mouse lung, using NBP3-21666.](http://images.novusbio.com/fullsize/nbp3-21666_rabbit-runx1-2-3-mab-sr1906-6720239533744.jpg "Immunohistochemistry: RUNX1/2/3 Antibody (SR1906) [NBP3-21666] - Immunohistochemical analysis of paraffin-embedded mouse lung, using NBP3-21666.")
![Western Blot: RUNX1/2/3 Antibody (SR1906) [NBP3-21666] - Western blot analysis using NBP3-21666 in MOLT4 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21666_rabbit-runx1-2-3-mab-sr1906-6720239533727.jpg)
![Western Blot: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Western blot detection of Lamin A + C in 3T3, Hela cell lysates using NBP3-19963 (1:1000 diluted). Predicted band size: 74kDa. Observed band size: 74, 63kDa.](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-S06-7H5-Western-Blot-NBP3-19963-img0003.jpg "Western Blot: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Western blot detection of Lamin A + C in 3T3, Hela cell lysates using NBP3-19963 (1:1000 diluted). Predicted band size: 74kDa. Observed band size: 74, 63kDa.")
![Immunohistochemistry-Paraffin: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Immunohistochemistry of Lamin A + C in paraffin-embedded Human breast cancer tissue using NBP3-19963 at dilution 1/5](http://images.novusbio.com/fullsize/Lamin-A-+-C-Antibody-S06-7H5-Immunohistochemistry-Paraffin-NBP3-19963-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lamin A + C Antibody (S06-7H5) [NBP3-19963] - Immunofluorescence of Lamin A + C (green) in Hela cells using NBP3-19963 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19963_rabbit-lamin-a-c-mab-s06-7h5-113202515145450.jpg)
![Western Blot: sFRP-5 Antibody [NBP3-12343] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 38 KDa.](http://images.novusbio.com/fullsize/sFRP-5-Antibody-Western-Blot-NBP3-12343-img0005.jpg "Western Blot: sFRP-5 Antibody [NBP3-12343] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 38 KDa.")
![Immunohistochemistry-Paraffin: sFRP-5 Antibody [NBP3-12343] - Mouse Small Intestine. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/sFRP-5-Antibody-Immunohistochemistry-Paraffin-NBP3-12343-img0004.jpg)
![Immunohistochemistry-Paraffin: sFRP-5 Antibody [NBP3-12343] - Mouse Testis. 1:100 dilution IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 20X magnification.](http://images.novusbio.com/fullsize/sFRP-5-Antibody-Immunohistochemistry-Paraffin-NBP3-12343-img0001.jpg)
![Immunohistochemistry-Paraffin: sFRP-5 Antibody [NBP3-12343] - Mouse Testis. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/sFRP-5-Antibody-Immunohistochemistry-Paraffin-NBP3-12343-img0002.jpg)
![Western Blot: IL-33 Antibody (IL33305B) [NBP3-11681] - Western blot analysis of human IL-33 using IL-33 antibody (IL33305B) at 1:2,000 dilution.1. Recombinant human IL-33 (His tagged).2. Recombinant human IL-33 (FLAG(R)-tagged).3. Recombinant mouse IL-33 (FLAG(R)-tagged).4. Other protein (His tagged, negative control).](http://images.novusbio.com/fullsize/IL-33-Antibody-IL33305B-Western-Blot-NBP3-11681-img0003.jpg "Western Blot: IL-33 Antibody (IL33305B) [NBP3-11681] - Western blot analysis of human IL-33 using IL-33 antibody (IL33305B) at 1:2,000 dilution.1. Recombinant human IL-33 (His tagged).2. Recombinant human IL-33 (FLAG(R)-tagged).3. Recombinant mouse IL-33 (FLAG(R)-tagged).4. Other protein (His tagged, negative control).")
![Immunohistochemistry: IL-33 Antibody (IL33305B) [NBP3-11681] - Immunohistochemical staining of human tissue using IL-33 antibody (IL33305B) at 1:100 dilution.A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown colour).B. Isotype control with IgG2a (negative control).](http://images.novusbio.com/fullsize/IL-33-Antibody-IL33305B-Immunohistochemistry-NBP3-11681-img0001.jpg)
![Immunoprecipitation: IL-33 Antibody (IL33305B) [NBP3-11681] - Immunoprecipitation of recombinant hIL-33 proteins using IL-33 antibody (IL33305B). Recombinant hIL-33 Proteins were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot/ECL reaction with anti-FLAG or anti-His.](http://images.novusbio.com/fullsize/IL-33-Antibody-IL33305B-Immunoprecipitation-NBP3-11681-img0002.jpg)
![Western Blot: SOX4 Antibody [NBP3-18643] - Figure 4. IP using the antibody directed against SOX4 IP was performed overnight on protein extracts (600 ug) from CT-2A (A) and GL261 (B) cells using 6 ul of the antibody against SOX4 . The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 2 shows the result of the IP; the input is shown in lane 1.](http://images.novusbio.com/fullsize/SOX4-Antibody-Western-Blot-NBP3-18643-img0003.jpg "Western Blot: SOX4 Antibody [NBP3-18643] - Figure 4. IP using the antibody directed against SOX4 IP was performed overnight on protein extracts (600 ug) from CT-2A (A) and GL261 (B) cells using 6 ul of the antibody against SOX4 . The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 2 shows the result of the IP; the input is shown in lane 1.")
![Western Blot: SOX4 Antibody [NBP3-18643] - Figure 2. Western blot analysis using the antibody directed against SOX4 A. H1299 lung carcinoma cells were transfected with HA-tagged SOX4 (lane 1) or with a HA-tagged truncated SOX4 (395X mutant, lane 2). Whole cell extracts (60 - 100 ug) were analysed by Western blot using the antibody against SOX4 diluted 1:4,000 in TBS-Tween containing 2% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. B. Whole cell extracts of 4 different lung carcinoma cell lines were analysed by Western blot with the antibody against SOX4 as described above. The H522 cells are known to overexpress SOX4. Western blot performed by Montse Sanchez-Cespedes and Sandra Castillo Diez, Catalan Institute of Oncology, Barcelona, Spain.](http://images.novusbio.com/fullsize/SOX4-Antibody-Western-Blot-NBP3-18643-img0001.jpg)
![Western Blot: SOX4 Antibody [NBP3-18643] - Figure 3. Western blot analysis using the antibody directed against SOX4 Western blot was performed on Protein extracts (~40 ug) from mouse B16-OVA (A), CT-2A (B), GL261 (C) or B16A (D) cells using the antibody against SOX4 . The cells were transfected with a SOX4 specific crRNA (lanes 2) or with a negative control crRNA (lanes 1). The antibody was diluted 1:4,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right. The lower panel shows the WB results with a GAPDH antibody used as loading control. Western blot performed by Anze Godicelj, Dana Farber Cancer Institute, Boston, USA.](http://images.novusbio.com/fullsize/SOX4-Antibody-Western-Blot-NBP3-18643-img0002.jpg)
![ELISA: SOX4 Antibody [NBP3-18643] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human SOX4 . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:18,000.](http://images.novusbio.com/fullsize/SOX4 Antibody-ELISA-NBP3-18643-img0004.jpg)
![Western Blot: sFRP-3/FRZB Antibody [H00002487-D01P] - Analysis of FRZB expression in mouse liver.](http://images.novusbio.com/fullsize/sFRP-3-FRZB-Antibody-Western-Blot-H00002487-D01P-img0007.jpg "Western Blot: sFRP-3/FRZB Antibody [H00002487-D01P] - Analysis of FRZB expression in mouse liver.")
![Western Blot: sFRP-3/FRZB Antibody [H00002487-D01P] - Analysis of FRZB expression in PC-12.](http://images.novusbio.com/fullsize/sFRP-3-FRZB-Antibody-Western-Blot-H00002487-D01P-img0004.jpg)
![Western Blot: sFRP-3/FRZB Antibody [H00002487-D01P] - Analysis of FRZB expression in Raw 264.7.](http://images.novusbio.com/fullsize/sFRP-3-FRZB-Antibody-Western-Blot-H00002487-D01P-img0005.jpg)
![Western Blot: sFRP-3/FRZB Antibody [H00002487-D01P] - Analysis of FRZB expression in NIH/3T3.](http://images.novusbio.com/fullsize/sFRP-3-FRZB-Antibody-Western-Blot-H00002487-D01P-img0006.jpg)
![Western Blot: sFRP-3/FRZB Antibody [H00002487-D01P] - Analysis of FRZB expression in transfected 293T cell line by FRZB polyclonal antibody.Lane 1: FRZB transfected lysate(36.30 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/sFRP-3-FRZB-Antibody-Western-Blot-H00002487-D01P-img0008.jpg)
![Immunohistochemistry-Paraffin: OFD1 Antibody [NBP1-89354] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/OFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89354-img0005.jpg "Immunohistochemistry-Paraffin: OFD1 Antibody [NBP1-89354] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry: OFD1 Antibody [NBP1-89354] - Staining of human prostate shows distinct cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/OFD1-Antibody-Immunohistochemistry-NBP1-89354-img0002.jpg)
![Immunohistochemistry-Paraffin: OFD1 Antibody [NBP1-89354] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/OFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89354-img0003.jpg)
![Immunohistochemistry-Paraffin: OFD1 Antibody [NBP1-89354] - Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/OFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89354-img0004.jpg)
![Immunohistochemistry-Paraffin: OFD1 Antibody [NBP1-89354] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/OFD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89354-img0006.jpg)
![Immunohistochemistry-Paraffin: HHATL Antibody [NBP1-86791] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/HHATL-Antibody-Immunohistochemistry-Paraffin-NBP1-86791-img0010.jpg "Immunohistochemistry-Paraffin: HHATL Antibody [NBP1-86791] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.")
![Immunohistochemistry-Paraffin: HHATL Antibody [NBP1-86791] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/HHATL-Antibody-Immunohistochemistry-Paraffin-NBP1-86791-img0006.jpg)
![Immunohistochemistry-Paraffin: HHATL Antibody [NBP1-86791] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/HHATL-Antibody-Immunohistochemistry-Paraffin-NBP1-86791-img0007.jpg)
![Immunohistochemistry-Paraffin: HHATL Antibody [NBP1-86791] - Staining of human skeletal muscle shows weak to moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/HHATL-Antibody-Immunohistochemistry-Paraffin-NBP1-86791-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HHATL Antibody [NBP1-86791] - Staining in human heart muscle and prostate tissues.. Corresponding HHATL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HHATL-Antibody-Immunohistochemistry-Paraffin-NBP1-86791-img0009.jpg)