| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37393-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61722-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-12168-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11971-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75900SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47744-0.1mg | |||
| IHC-Prediluted | NBP2-48302 | |||
![Western Blot: MRPL42 Antibody (3H6G11) [NBP2-37393] - Western blot analysis using MRPL42 mouse mAb against HL7702 (1), HepG2 (2), SMMC-7721 (3), HEK293 (4), HeLa (5), and Raji (6) cell lysate.](http://images.novusbio.com/fullsize/MRPL42-Antibody-3H6G11-Western-Blot-NBP2-37393-img0004.jpg "Western Blot: MRPL42 Antibody (3H6G11) [NBP2-37393] - Western blot analysis using MRPL42 mouse mAb against HL7702 (1), HepG2 (2), SMMC-7721 (3), HEK293 (4), HeLa (5), and Raji (6) cell lysate.")
![Immunocytochemistry/Immunofluorescence: MRPL42 Antibody (3H6G11) [NBP2-37393] - Immunofluorescence analysis of HeLa cells using MRPL42 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/MRPL42-Antibody-3H6G11-Immunocytochemistry-Immunofluorescence-NBP2-37393-img0005.jpg)
![Immunohistochemistry: MRPL42 Antibody (3H6G11) [NBP2-37393] - Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MRPL42 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MRPL42-Antibody-3H6G11-Immunohistochemistry-NBP2-37393-img0008.jpg)
![Flow Cytometry: MRPL42 Antibody (3H6G11) [NBP2-37393] - Flow cytometric analysis of HepG2 cells using MRPL42 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/MRPL42-Antibody-3H6G11-Flow-Cytometry-NBP2-37393-img0006.jpg)
![Western Blot: MRPL42 Antibody (3H6G11) [NBP2-37393] - Western blot analysis using MRPL42 mAb against human MRPL42 recombinant protein. (Expected MW is 37.9 kDa)](http://images.novusbio.com/fullsize/MRPL42-Antibody-3H6G11-Western-Blot-NBP2-37393-img0002.jpg)
![Western Blot: MRPL42 Antibody (3H6G11) [NBP2-37393] - Western blot analysis using MRPL42 mAb against HEK293 (1) and MRPL42 (AA: 142-203)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/MRPL42-Antibody-3H6G11-Western-Blot-NBP2-37393-img0003.jpg)
![Immunohistochemistry: MRPL42 Antibody (3H6G11) [NBP2-37393] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using MRPL42 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/MRPL42-Antibody-3H6G11-Immunohistochemistry-NBP2-37393-img0007.jpg)
![ELISA: MRPL42 Antibody (3H6G11) [NBP2-37393] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/MRPL42-Antibody-3H6G11-ELISA-NBP2-37393-img0001.jpg)
![Western Blot: Catenin alpha 1 Antibody (8B6C1) [NBP2-61722] - Analysis using CTNNA1 mAb against human CTNNA1 (AA: 371-574) recombinant protein. (Expected MW is 48.8 kDa)](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-8B6C1-Western-Blot-NBP2-61722-img0001.jpg "Western Blot: Catenin alpha 1 Antibody (8B6C1) [NBP2-61722] - Analysis using CTNNA1 mAb against human CTNNA1 (AA: 371-574) recombinant protein. (Expected MW is 48.8 kDa)")
![Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody (8B6C1) [NBP2-61722] - Analysis of paraffin-embedded stomach tissues using CTNNA1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-8B6C1-Immunohistochemistry-Paraffin-NBP2-61722-img0003.jpg)
![Western Blot: Catenin alpha 1 Antibody (8B6C1) [NBP2-61722] - Analysis using CTNNA1 mAb against HEK293 (1) and CTNNA1 (AA: 371-574)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-8B6C1-Western-Blot-NBP2-61722-img0002.jpg)
![ELISA: Catenin alpha 1 Antibody (8B6C1) [NBP2-61722] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/Catenin-alpha-1-Antibody-8B6C1-ELISA-NBP2-61722-img0004.jpg)
![Flow Cytometry: CD8 Antibody (fCD8) [NBP1-28254] - CD8 alpha Antibody (fCD8) [NBP1-28254] - Analysis using the PE conjugate of NBP1-28254. Multiple staining of peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-fCD8-Flow-Cytometry-NBP1-28254-img0003.jpg "Flow Cytometry: CD8 Antibody (fCD8) [NBP1-28254] - CD8 alpha Antibody (fCD8) [NBP1-28254] - Analysis using the PE conjugate of NBP1-28254. Multiple staining of peripheral blood lymphocytes.")
![Flow Cytometry: CD8 Antibody (fCD8) [NBP1-28254] - CD8 alpha Antibody (fCD8) [NBP1-28254] - Flow Cytometry: CD8 alpha Antibody (fCD8) [Alexa Fluor® 405] [NBP1-28254AF405] - T-cell detection in feline whole blood. Image from verified customer review. Image using the Alexa Fluor 405 form of this antibody.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-fCD8-Flow-Cytometry-NBP1-28254-img0005.jpg)
![Flow Cytometry: CD8 alpha Antibody (fCD8) [NBP1-28254] - Analysis using the PE conjugate of NBP1-28254. Double staining of CD8 alpha Antibody (fCD8) [PE] 0.2 ug/10^6 Peripheral blood mononuclear cells from a healthy domestic cat with mouse anti-feline CD5-FITC and mouse anti-feline CD8-R-PE.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-fCD8-Flow-Cytometry-NBP1-28254-img0004.jpg)
![Immunohistochemistry-Paraffin: B220/CD45R Antibody (RM0063-9F14) [NBP2-12168] - Staining of paraffin sections of mouse spleen from LPS exposed animals.](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-RM0063-9F14-Immunohistochemistry-Paraffin-NBP2-12168-img0002.jpg "Immunohistochemistry-Paraffin: B220/CD45R Antibody (RM0063-9F14) [NBP2-12168] - Staining of paraffin sections of mouse spleen from LPS exposed animals.")
![Immunohistochemistry: B220/CD45R Antibody (RM0063-9F14) [NBP2-12168] - Negative control (left) and rat anti mouse CD45R (right).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-RM0063-9F14-Immunohistochemistry-NBP2-12168-img0001.jpg)
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0005.jpg "Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R))488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Flow cytometric analysis of ICAM-1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-6, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a FITC-conjugated Goat anti-Mouse IgG Secondary antibody at 1/200 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Flow-Cytometry-NBP2-66896-img0004.jpg)
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: Raji cell lysateLane 2: Daudi cell lysate](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0001.jpg)
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue)](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0002.jpg)
![Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024158265.jpg "Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds")
![Immunohistochemistry-Frozen: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33094, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024159390.jpg)
![Immunohistochemistry: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-298202414161822.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunocytochemistry analysis of C6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202410234261.jpg)
![Flow Cytometry: Vimentin Antibody (SC60-05) [NBP3-33094] - Flow cytometric analysis of HeLa (positive, red) and MCF7 (negative, green) cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33094) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HeLa black, MCF7 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202416453228.jpg)
![Western Blot: Vimentin Antibody (SC60-05) [NBP3-33094] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Lane 6: C6 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-3920248453527.jpg)
![Western Blot: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Analysis of FABP4 on mouse heart tissue lysates using anti-FABP4 antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-JM10-99-Western-Blot-NBP2-66828-img0005.jpg "Western Blot: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Analysis of FABP4 on mouse heart tissue lysates using anti-FABP4 antibody at 1/500 dilution.")
![Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Staining FABP4 in PMVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-JM10-99-Immunocytochemistry-Immunofluorescence-NBP2-66828-img0006.jpg)
![Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Analysis of paraffin-embedded mouse lung tissue using anti-FABP4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-JM10-99-Immunohistochemistry-Paraffin-NBP2-66828-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Staining FABP4 in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-JM10-99-Immunocytochemistry-Immunofluorescence-NBP2-66828-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Staining FABP4 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-JM10-99-Immunocytochemistry-Immunofluorescence-NBP2-66828-img0002.jpg)
![Immunohistochemistry-Paraffin: FABP4/A-FABP Antibody (JM10-99) [NBP2-66828] - Analysis of paraffin-embedded human breast tissue using anti-FABP4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FABP4-A-FABP-Antibody-JM10-99-Immunohistochemistry-Paraffin-NBP2-66828-img0003.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Western-Blot-NBP2-66756-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Western blot analysis of CD40 Ligand/TNFSF5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse liver tissue lysate Lane 2: rat liver tissue lysate Lane 2: mouse bone marrow tissue lysate")
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0006.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of paraffin-embedded human spleen tissue using anti- CD40L antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunohistochemistry-Paraffin-NBP2-66756-img0004.jpg)
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Analysis of THP-1 cells with CD40L antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Flow-Cytometry-NBP2-66756-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (JM11-34) [NBP2-66756] - Staining CD40L in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-JM11-34-Immunocytochemistry-Immunofluorescence-NBP2-66756-img0003.jpg)
![Flow Cytometry: Podoplanin Antibody (PMab-1) - Chimeric [NBP3-11971] - NIH3T3 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of PMab-1 (NBP3-11971, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Podoplanin-Antibody-PMab-1-Chimeric-Flow-Cytometry-NBP3-11971-img0001.jpg "Flow Cytometry: Podoplanin Antibody (PMab-1) - Chimeric [NBP3-11971] - NIH3T3 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of PMab-1 (NBP3-11971, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Western Blot: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] - Podoplanin (MAP tag) expressing plasmid transfected (A) and non-transfected (B) HEK293 cells lysate (3ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP3-11971 at 0.00001ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-11971_rabbit-monoclonal-podoplanin-antibody-pmab-1-chimeric-74202522113027.png)
![Immunocytochemistry /Immunofluorescence: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells transfected with Podoplanin (MAP tag) expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton stained with NBP3-11971 (1:200 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-11971_rabbit-monoclonal-podoplanin-antibody-pmab-1-chimeric-74202522113019.png)
![Western Blot: Podoplanin Antibody (PMab-1) - Chimeric - Azide and BSA Free [NBP3-11971] - Mouse thymus (A), mouse brain (B), NIH3T3 cells (C) and mouse kidney (D) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with NBP3-11971 at 0.01 ug/ml, 0.1 ug/ml, 0.001 ug/ml and 0.003 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-11971_rabbit-monoclonal-podoplanin-antibody-pmab-1-chimeric-74202522141240.png)
![Immunohistochemistry: CD4 Antibody (YNB46.1.8 (Campath-9H)) [NBP2-75900] - Anti-CD4 staining of formaldehyde fixed paraffin embedded rabbit spleen tissue, at 40x magnification. The rat IgG1-chimeric version of YNB46.1.8 (NBP2-75900) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/CD4-Antibody-YNB46-1-8-Campath-9H-Immunohistochemistry-NBP2-75900-img0001.jpg "Immunohistochemistry: CD4 Antibody (YNB46.1.8 (Campath-9H)) [NBP2-75900] - Anti-CD4 staining of formaldehyde fixed paraffin embedded rabbit spleen tissue, at 40x magnification. The rat IgG1-chimeric version of YNB46.1.8 (NBP2-75900) was used to stain samples at a concentration of 5 ug/ml.")
![Western Blot: CD4 Antibody (YNB46.1.8 (Campath-9H)) - Azide and BSA Free [NBP2-75900] - Mouse thymus (lane 1) and mouse spleen (lane 2) were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75900 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (51 kDa) is seen along with bands corresponding to dimers.](http://images.novusbio.com/fullsize/nbp2-75900_rat-monoclonal-cd4-antibody-ynb46-1-8-campath-9h-7420252211305.png)
![Immunocytochemistry/Immunofluorescence: APRT Antibody [NBP1-89519] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/APRT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89519-img0006.jpg "Immunocytochemistry/Immunofluorescence: APRT Antibody [NBP1-89519] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.")
![Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling IDH1. Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1/1152) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-IDH1-1152-Immunocytochemistry-Immunofluorescence-NBP2-45156-img0004.jpg "Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling IDH1. Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1/1152) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.")
![Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Isocitrate Dehydrogenase 1/IDH1 Mouse Monoclonal Antibody (Isocitrate Dehydrogenase 1/IDH1/1152).](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-IDH1-1152-Immunohistochemistry-Paraffin-NBP2-45156-img0003.jpg)
![Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] - Human Colon Carcinoma stained with IDH1 Monoclonal Antibody (IDH1/1152).](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-IDH1-1152-Immunohistochemistry-Paraffin-NBP2-45156-img0001.jpg)
![Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/1152) [NBP2-45156] - Human Prostate Carcinoma stained with IDH1 Monoclonal Antibody (IDH1/1152).](http://images.novusbio.com/fullsize/nbp2-45156_mouse-monoclonal-isocitrate-dehydrogenase-1-idh1-antibody-idh1-1152-17720241044632.jpg)
