Stem Cell Markers reagents: Novus Biologicals

NBP1-97725

Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free

(1 Review)

(2 Publications)

0.1 mg

NBP1-97725

Immunocytochemistry/Immunofluorescence: Actin Alpha 1 Cardiac Muscle Antibody (22D3) [NBP1-97725] - Immunofluorescence staining of muscle tissue in the tail of 3 days old zebrafish embryo

Reactivity:

Hu, Mu, Rt, Po, Bv, Gt, Rb, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Gt, Rb, Ze

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-97725AF350
Alexa Fluor 405	NBP1-97725AF405
Alexa Fluor 488	NBP1-97725AF488
Alexa Fluor 532	NBP1-97725AF532
Alexa Fluor 594	NBP1-97725AF594
Alexa Fluor 647	NBP1-97725AF647
Alexa Fluor 700	NBP1-97725AF700
Alexa Fluor 750	NBP1-97725AF750
Alexa Fluor Plus 488	NBP1-97725AFP488
Alexa Fluor Plus 555	NBP1-97725AFP555
Alexa Fluor Plus 594	NBP1-97725AFP594
Alexa Fluor Plus 647	NBP1-97725AFP647
Alexa Fluor Plus 680	NBP1-97725AFP680
Allophycocyanin	NBP1-97725APC
Biotin	NBP1-97725B
CoraFluor 1	NBP1-97725CL1
DyLight 350	NBP1-97725UV
DyLight 405	NBP1-97725V
DyLight 488	NBP1-97725G
DyLight 550	NBP1-97725R
DyLight 594	NBP1-97725DL594
DyLight 650	NBP1-97725C
DyLight 680	NBP1-97725FR
DyLight 755	NBP1-97725IR
FITC	NBP1-97725F
HRP	NBP1-97725H
Janelia Fluor 525	NBP1-97725JF525
Janelia Fluor 549	NBP1-97725JF549
Janelia Fluor 646	NBP1-97725JF646
Janelia Fluor 669	NBP1-97725JF669
PE	NBP1-97725PE
PerCP	NBP1-97725PCP
mFluor Violet 500 SE	NBP1-97725MFV500
mFluor Violet 610 SE	NBP1-97725MFV610

compare

(select up to 3 products from this page to compare)

NBP1-42825

Human ESC Marker Antibody Pack

9 Vials

NBP1-42825

Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - Analysis of human embryonic stem cells stained with SSEA-3 antibody detected with Alexa Fluor 488 anti-rat IgM secondary antibody. SSEA-3 Antibody (MC-631) [NB100-1832]

<b>Independent Antibodies Validation. </b>Immunoprecipitation: Human ESC Marker Antibody Pack [NBP1-42825] - Detection of Mouse Nanog on HeLa whole cell lysate using NB100-588. Nanog was also IPed using NB100-587 and another Nanog. IP reactions were controlled using normal rabbit IgG. IPed Nanog was blotted using NB100-587.

Western Blot: Human ESC Marker Antibody Pack [NBP1-42825] - Whole cell protein from human ES cells (lane 1), human NTERA-2 cells (lane 2), mouse embryonic fibroblasts (MEF, lane 3) and mouse V6.5 ES cells (lane 4, NBP1-97433) were separated on a 7.5% gel and transferred to PVDF membrane. The membrane was then incubated with anti-TRA1-60(R) antibody at 2 ug/ml and detected with an anti-mouse IgM secondary antibody using chemiluminescence. Note how this antibody specifically detects the TRA1-60(R) epitope only on human cells. TRA-1-60 (R) Antibody (TRA-1-60(R)) [NB100-730]

Immunocytochemistry/Immunofluorescence: Human ESC Marker Antibody Pack [NBP1-42825] - SSEA1 antibody was tested in Ntera-2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red). SSEA-1 Antibody (MC-480) [NB100-1831]

Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS analysis of Nanog expression in primary mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.

Western Blot: Human ESC Marker Antibody Pack [NBP1-42825] - Detection of OCT4 in mouse brain lysate

Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS staining of NTERA-2 cells using NB100-1831 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-mouse IgM secondary antibody.

Flow Cytometry: Human ESC Marker Antibody Pack [NBP1-42825] - FACS staining of NTERA-2 cells using NB100-2379 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP2-15052

OCT4 Antibody (486) - Azide and BSA Free

(1 Review)

0.1 ml

NBP2-15052

Flow Cytometry: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Oct4 antibody dilution: 1:50

Western Blot: OCT4 Antibody (486) [NBP2-15052] - Sample (10 ug of whole cell lysate) A: Non-transfected HeLa lysates B: HeLa transfected recombinant OCT3/4 protein 10% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Human embryonic stem cell were fixed in 4% Paraformaldehyde for 15 min. Green: Oct4 protein stained by Oct4 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - BT483 xenograft, using OCT3/4 antibody at 1:200 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Imaging of Rabbit stem cels in Bone marrow of tibia. This image was submitted via customer Review.

Immunoprecipitation: OCT4 Antibody (486) [NBP2-15052] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune mouse IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.

Western Blot: OCT4 Antibody (486) [NBP2-15052] - Mouse embryonic stem cell extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] (NBP2-15052) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: OCT4 Antibody (486) [NBP2-15052] - Oct4 antibody [GT486] detects Oct4 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Oct4 stained by Oct4 antibody [GT486] (NBP2-15052) diluted at 1:500.Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.

<b>Genetic Strategies Validation. </b>Western Blot: OCT4 Antibody (486) [NBP2-15052] - Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody [GT486] diluted at 1:120000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Oct4 antibody [GT486] detects Oct4 protein by western blot analysis.A. 30 ug human ESC whole cell lysate/extract10% SDS-PAGE (NBP2-15052) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rb, Pm, RM

Species Glossary

Reactivity: Hu, Mu, Rb, Pm, RM

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP2-19711

Pax6 Antibody - BSA Free

(4 Publications)

0.1 ml

NBP2-19711

Immunohistochemistry-Paraffin: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: Pax6 Antibody [NBP2-19711] - Pax6 antibody detects Pax6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Pax6 stained by Pax6 antibody (NBP2-19711) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: PAX6 stained by PAX6 antibody (NBP2-19711) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Hoechst 33342 staining.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PAX6 antibody detects PAX6 protein at nucleus in embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE12.5). PAX6 antibody (NBP2-19711) diluted at 1:250.

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: PAX6 stained by PAX6 antibody (NBP2-19711) diluted at 1:200.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Samples: Paraffin-Embedded mouse retina.Green: PAX6 protein stained by PAX6 antibody (NBP2-19711) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500.Blue: Fluoroshield with DAPI . br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PAX6 antibody detects PAX6 protein at nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PAX6 protein stained by PAX6 antibody (NBP2-19711) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.Scale bar = 10 um.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (NBP2-19711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Ch, Rb, RM, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Rb, RM, Xp

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP2-21569

Cytochrome c Antibody

(4 Publications)

0.1 ml

NBP2-21569

Western Blot: Cytochrome C Antibody [NBP2-21569] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.

Western Blot: Cytochrome c Antibody [NBP2-21569] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident pico Western HRP Substrate.

Immunohistochemistry-Paraffin: Cytochrome c Antibody [NBP2-21569] - Human cervical carcinoma. Cytochrome C stained by Cytochrome C antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Simple Western: Cytochrome c Antibody [NBP2-21569] - Cytochrome C Antibodies (NBP2-21569), ProteinSimple Western Blot on Jess Instrument; 1 microgram of human brain tissue lysate was tested with the antibodies diluted 10 and 20 times. Image from verified customer review.

Western Blot: Cytochrome c Antibody [NBP2-21569] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome c antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Orthogonal Strategies Validation. </b>Western Blot: Cytochrome c Antibody [NBP2-21569] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody (NBP2-21569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, Ca, Dr, Po, Bv, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Dr, Po, Bv, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

(select up to 3 products from this page to compare)

NBP1-30975

hnRNP K Antibody

(1 Review)

100 ul

NBP1-30975

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.

Western Blot: hnRNP K Antibody [NBP1-30975] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded mouse urinary bladder. hnRNP K antibody dilution: 1:500.

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded mouse colon. hnRNP K antibody dilution: 1:500.

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - Paraffin-embedded rat brain stem. hnRNP K antibody dilution: 1:500.

Immunohistochemistry-Paraffin: hnRNP K Antibody [NBP1-30975] - hnRNP K antibody detects hnRNP K protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.hnRNP K stained by hnRNP K antibody (NBP1-30975) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca, Rb, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Rb, RM

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP2-29455

CD34 Antibody (ICO-115)

(1 Review)

(8 Publications)

0.1 mg

NBP2-29455

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

ABX-114A

Alt Catalog# ABX-114A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-33076AF350
Alexa Fluor 405	NBP2-33076AF405
Alexa Fluor 488	NBP2-33076AF488
Alexa Fluor 532	NBP2-33076AF532
Alexa Fluor 594	NBP2-33076AF594
Alexa Fluor 647	NBP2-33076AF647
Alexa Fluor 700	NBP2-33076AF700
Alexa Fluor 750	NBP2-33076AF750
Alexa Fluor Plus 488	NBP2-33076AFP488
Alexa Fluor Plus 555	NBP2-33076AFP555
Alexa Fluor Plus 594	NBP2-33076AFP594
Alexa Fluor Plus 647	NBP2-33076AFP647
Alexa Fluor Plus 680	NBP2-33076AFP680
Allophycocyanin	NBP2-33076APC
Allophycocyanin/Cy7	NBP2-33076APCCY7
Biotin	NBP2-33076B
CoraFluor 1	NBP2-33076CL1
DyLight 350	NBP2-33076UV
DyLight 405	NBP2-33076V
DyLight 488	NBP2-33076G
DyLight 550	NBP2-33076R
DyLight 594	NBP2-33076DL594
DyLight 650	NBP2-33076C
DyLight 680	NBP2-33076FR
DyLight 755	NBP2-33076IR
FITC	NBP2-33076F
HRP	NBP2-33076H
Janelia Fluor 525	NBP2-33076JF525
Janelia Fluor 549	NBP2-33076JF549
Janelia Fluor 646	NBP2-33076JF646
Janelia Fluor 669	NBP2-33076JF669
PE	NBP2-33076PE
PE/Atto594	NBP2-33076PEATT594
PE/Cy5.5	NBP2-33076PECY55
PE/Cy7	NBP2-33076PECY7
PerCP	NBP2-33076PCP
mFluor Violet 450 SE	NBP2-33076MFV450
mFluor Violet 500 SE	NBP2-33076MFV500
mFluor Violet 610 SE	NBP2-33076MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-33076

compare

(select up to 3 products from this page to compare)

NBP1-85814

Vimentin Antibody - BSA Free

(4 Publications)

0.1 ml

NBP1-85814

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NBP1-85814] - Staining of human cell line ASC TERT1 shows localization to intermediate filaments. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human cervix, uterine shows moderate cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human skeletal muscle shows no positivity in myocytes.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human ovary shows moderate cytoplasmic positivity in stromal cells.

Immunohistochemistry-Paraffin: Vimentin Antibody [NBP1-85814] - Staining of human fallopian tube shows moderate membranous positivity in glandular cells.

Simple Western: Vimentin Antibody [NBP1-85814] - Simple Western lane view shows a specific band for VIM in 0.2 mg/ml of U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Vimentin Antibody [NBP1-85814] - Electropherogram image(s) of corresponding Simple Western lane view. Vimentin antibody was used at 1:20 dilution on NIH-3T3 lysates(s).

Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Analysis in human cell lines U-251MG and MCF-7 using Anti-VIM antibody. Corresponding VIM RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-85814-25ul

compare

(select up to 3 products from this page to compare)

NBP1-82797

MAT2B Antibody - BSA Free

(1 Review)

(3 Publications)

0.1 ml

NBP1-82797

Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797] - Staining of human kidney shows moderate cytoplasmic and nuclear positivity in cells in tubules.

Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797] - Staining of human duodenum shows moderate positivity in luminal membrane in glandular cells.

Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797] - Staining of human liver shows moderate positivity in bile canaliculi in hepatocytes.

Immunohistochemistry-Paraffin: MAT2B Antibody [NBP1-82797] - Staining of human cerebral cortex shows moderate positivity in neuronal processes in astrocytes.

MAT1A, MAT2A, MAT2B and miR-203 expression in human HCC with different prognosis(A) MAT1A, MAT2A, MAT2B, Ki67 and MDK gene expression. The results are expressed as N-fold differences in target gene expression relative to the RNR-18 expression, named N Target (NT). NT = 2– delta CT, delta CT of each sample was calculated by subtracting the Ct of the target gene from the Ct of the RNR-18 gene. (B) Representative Western Blot of MAT alpha 1, MAT alpha 2, and MAT beta 2, in HCCs with different prognosis and correspondent surroundings. Chemiluminescence analysis: optical densities were normalized to beta -actin levels and expressed in arbitrary units. (C) miR-203 expression (NT = 2– delta CT) and Spearman’s correlation analysis of miR-203. A total of 26 cases (13 HCCB and 13 HCCP) were used for the correlation analysis. Data are means +/- standard deviation (SD) of six experiments. Mann-Whitney test: Point, different from NL for P 3 years) and correspondent surrounding (SLB); HCCP, HCC with poorer prognosis (survival < 3 years) and corresponding surrounding (SLP). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31073374), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Rt, Mu

Species Glossary

Reactivity: Hu, Rt, Mu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-82797-25ul

compare

(select up to 3 products from this page to compare)

NBP1-87781

Myelin PLP Antibody - BSA Free

(1 Review)

(6 Publications)

0.1 ml

NBP1-87781

Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human cerebral cortex shows high expression.

Immunohistochemistry-Paraffin: Myelin PLP Antibody [NBP1-87781] - Staining of human pancreas shows low expression as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-87781-25ul

compare

(select up to 3 products from this page to compare)

NBP2-02577

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A is involved in actin & profilin nuclear export processHeLa cells treated with control or RASSF1A siRNA were fractionated into cytoplasmic & nuclear extracts. Lysates from each fraction were probed for actin & profilin alongside GAPDH (as a marker of the cytoplasmic fraction) & Lamin A/C (as a marker of the nuclear fraction). Right: quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from two independent experiments & represent the SEM.Immunofluorescence images of profilin in control & RASSF1A siRNA‐transfected HeLa cells. Right: the profilin localisation was scored as nuclear/cytoplasmic or predominantly cytoplasmic in approximately 100 cells. Error bars derive from three independent experiments & represent the SEM.Confocal images of endogenous monomeric globular actin (G‐actin) in siCTRL & siRASSF1A cells using DNase I staining (Alexa Fluor 488‐conjugated, green). Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with control pcDNA3 or RASSF1A vector. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis. *P < 0.05, **P < 0.01.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

$Immunoprecipitation: Profilin 1 Antibody (OTI1D5) [NBP2-02577] - RASSF1A depletion does not affect nuclear F‐actin levelsWestern blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with truncated RASSF1A mutants.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRASSF1A & siRASSF1A together with XPO6 plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siRNA‐resistant FLAG‐RASSF1A plasmid.Western blot analysis of actin & profilin levels in nuclear & cytoplasmic fractions of cells transfected with siMST2. Quantification of nuclear actin & profilin relative to Lamin A/C is shown. Error bars derive from three independent experiments & represent the SEM.Western blot analysis of exportin‐6 (XPO6) & importin‐9 (IPO9) in the absence of RASSF1A. GAPDH was used as a loading control.Confocal images of endogenous filamentous actin (F‐actin) in siCTRL & siRASSF1A cells using phalloidin staining (Alexa Fluor 568‐conjugated, red). DNA was stained with DAPI. Scale bars = 10 μm.Western blot analysis of actin, profilin, GAPDH & Lamin A/C levels in nuclear & cytoplasmic fractions of MDA‐MB‐231 cells treated with DMSO or 5′‐aza‐dC. The graph shows the nuclear levels of actin & profilin relative to Lamin A/C in MDA‐MB‐231 cells expressing RASSF1A. Error bars derive from two independent experiments & represent the SEM.Data information: Two‐tailed Student's t‐test was used for statistical analysis.*P < 0.05, **P < 0.01, ***P < 0.001.Source data are available online for this figure. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31414556), licensed under a CC-BY license. Not internally tested by Novus Biologicals.$

Reactivity:

Hu, Mu, Rt, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Pm

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73605AF350
Alexa Fluor 405	NBP2-73605AF405
Alexa Fluor 488	NBP2-73605AF488
Alexa Fluor 532	NBP2-73605AF532
Alexa Fluor 594	NBP2-73605AF594
Alexa Fluor 647	NBP2-73605AF647
Alexa Fluor 700	NBP2-73605AF700
Alexa Fluor 750	NBP2-73605AF750
Alexa Fluor Plus 488	NBP2-73605AFP488
Alexa Fluor Plus 555	NBP2-73605AFP555
Alexa Fluor Plus 594	NBP2-73605AFP594
Alexa Fluor Plus 647	NBP2-73605AFP647
Alexa Fluor Plus 680	NBP2-73605AFP680
Allophycocyanin	NBP2-73605APC
Biotin	NBP2-73605B
CoraFluor 1	NBP2-73605CL1
DyLight 350	NBP2-73605UV
DyLight 405	NBP2-73605V
DyLight 488	NBP2-73605G
DyLight 550	NBP2-73605R
DyLight 594	NBP2-73605DL594
DyLight 650	NBP2-73605C
DyLight 680	NBP2-73605FR
DyLight 755	NBP2-73605IR
FITC	NBP2-73605F
HRP	NBP2-73605H
Janelia Fluor 525	NBP2-73605JF525
Janelia Fluor 549	NBP2-73605JF549
Janelia Fluor 646	NBP2-73605JF646
Janelia Fluor 669	NBP2-73605JF669
PE	NBP2-73605PE
PerCP	NBP2-73605PCP
mFluor Violet 450 SE	NBP2-73605MFV450
mFluor Violet 500 SE	NBP2-73605MFV500
mFluor Violet 610 SE	NBP2-73605MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73605

compare

(select up to 3 products from this page to compare)

NBP1-48291

PLK1 Antibody (OTI1D4)

(1 Review)

0.1 ml

NBP1-48291

Flow Cytometry: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells transfected with either PLK1 Human Tagged ORF Clone overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-PLK1 antibody , and then analyzed by flow cytometry (1:100).

Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PLK1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human pancreas tissue using anti-PLK1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human prostate tissue using anti-PLK1 mouse monoclonal antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73472AF350
Alexa Fluor 405	NBP2-73472AF405
Alexa Fluor 488	NBP2-73472AF488
Alexa Fluor 532	NBP2-73472AF532
Alexa Fluor 594	NBP2-73472AF594
Alexa Fluor 647	NBP2-73472AF647
Alexa Fluor 700	NBP2-73472AF700
Alexa Fluor 750	NBP2-73472AF750
Alexa Fluor Plus 488	NBP2-73472AFP488
Alexa Fluor Plus 555	NBP2-73472AFP555
Alexa Fluor Plus 594	NBP2-73472AFP594
Alexa Fluor Plus 647	NBP2-73472AFP647
Alexa Fluor Plus 680	NBP2-73472AFP680
Allophycocyanin	NBP2-73472APC
Biotin	NBP2-73472B
CoraFluor 1	NBP2-73472CL1
DyLight 350	NBP2-73472UV
DyLight 405	NBP2-73472V
DyLight 488	NBP2-73472G
DyLight 550	NBP2-73472R
DyLight 594	NBP2-73472DL594
DyLight 650	NBP2-73472C
DyLight 680	NBP2-73472FR
DyLight 755	NBP2-73472IR
FITC	NBP2-73472F
HRP	NBP2-73472H
Janelia Fluor 525	NBP2-73472JF525
Janelia Fluor 549	NBP2-73472JF549
Janelia Fluor 646	NBP2-73472JF646
Janelia Fluor 669	NBP2-73472JF669
PE	NBP2-73472PE
PerCP	NBP2-73472PCP
mFluor Violet 450 SE	NBP2-73472MFV450
mFluor Violet 500 SE	NBP2-73472MFV500
mFluor Violet 610 SE	NBP2-73472MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73472

compare

(select up to 3 products from this page to compare)

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Kit Type

Lysate Type

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Actin Alpha 1 Cardiac Muscle Antibody (22D3) - BSA Free

Human ESC Marker Antibody Pack

OCT4 Antibody (486) - Azide and BSA Free

Pax6 Antibody - BSA Free

Cytochrome c Antibody

hnRNP K Antibody

CD34 Antibody (ICO-115)

Vimentin Antibody - BSA Free

MAT2B Antibody - BSA Free

Myelin PLP Antibody - BSA Free

Profilin 1 Antibody (OTI1D5)

PLK1 Antibody (OTI1D4)