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![Immunohistochemistry-Paraffin: HUNK Antibody [NLS7095] - Analysis of anti-HUNK / B19 antibody with human breast, carcinoma.](http://images.novusbio.com/fullsize/HUNK-Antibody-Immunohistochemistry-Paraffin-NLS7095-img0002.jpg "Immunohistochemistry-Paraffin: HUNK Antibody [NLS7095] - Analysis of anti-HUNK / B19 antibody with human breast, carcinoma.")
![Immunohistochemistry-Paraffin: HUNK Antibody [NLS7095] - Analysis of anti-HUNK / B19 antibody with breast at 8 ug/ml.](http://images.novusbio.com/fullsize/HUNK-Antibody-Immunohistochemistry-Paraffin-NLS7095-img0001.jpg)
![Immunohistochemistry-Paraffin: TAO Kinase 1 Antibody - BSA Free [NBP1-50166] - Analysis of anti-TAOK1 antibody with human brain, cortex at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/TAO-Kinase-1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-50166-img0002.jpg "Immunohistochemistry-Paraffin: TAO Kinase 1 Antibody - BSA Free [NBP1-50166] - Analysis of anti-TAOK1 antibody with human brain, cortex at concentration 5 ug/ml.")
![Immunohistochemistry-Paraffin: TAO Kinase 1 Antibody - BSA Free [NBP1-50166] - Analysis of anti-TAOK1 antibody with human brain, cortex at concentration 5 ug/ml.](http://images.novusbio.com/fullsize/TAO-Kinase-1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NBP1-50166-img0001.jpg)
![Western Blot: CD3 zeta [p Tyr111] Antibody (EM-55) [NBP2-37709] - Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY111 mCD3zeta (clone EM-55).](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr111-Antibody-EM-55-Western-Blot-NBP2-37709-img0003.jpg "Western Blot: CD3 zeta [p Tyr111] Antibody (EM-55) [NBP2-37709] - Cell lyzates of HEK293T/17 cells transiently transfected with expression vectors harboring genes for mCD3zeta wild type and six different mCD3zeta mutants, where particular ITAM tyrosines were substituted with phenylalanines, were prepared. Subsequently the lysates (non-reducing conditions) were immunoblotted with mouse mAb anti-pY111 mCD3zeta (clone EM-55).")
![Western Blot: CD3 zeta [p tyr 111] Antibody (EM-55) [NBP2-37709] - Analysis in lysates of HEK293T/17 cells transiently transfected with expression vector harboring gene for mCD3zeta. Lane 1) Unstimulated cells, Lane 2) Pervanadate-stimulated cells](http://images.novusbio.com/fullsize/CD3-zeta-p-tyr-111-Antibody-EM-55-Western-Blot-NBP2-37709-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193] - Detection of phosphorylated CD3 zeta (EM-26; light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min. Total CD3 zeta indicated in green, actin cytoskeleton in red.](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr72-Antibody-EM-26-Immunocytochemistry-Immunofluorescence-NBP2-62193-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193] - Detection of phosphorylated CD3 zeta (EM-26; light blue) in immunological synapse formed between the lymph node naive T cells from AND TCR transgenic mice and DCEK cells loaded with MCC peptide, after 20 min. Total CD3 zeta indicated in green, actin cytoskeleton in red.")
![Western Blot: CD3 zeta [p Tyr72] Antibody (EM-26) [NBP2-62193] - Reactivity of the monoclonal antibodies EM-26 (anti-CD3 zeta phospho-Tyr72) and EM-17 (anti-CD3 zeta phospho-Tyr153) with phosphorylated particular human CD3 zeta mutants. The Y1F and Y6F mutatants lack phosphotyrosine 72 and 153, respectively.](http://images.novusbio.com/fullsize/CD3-zeta-p-Tyr72-Antibody-EM-26-Western-Blot-NBP2-62193-img0001.jpg)
![Western Blot: p38 alpha [p Tyr323] Antibody [NBP3-23249] - A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38 alpha (a.a. 319-328) (lanes 1 & 2) or anti-p38 alpha (Tyr-323) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23249_rabbit-p38-alpha-p-tyr323-pab-19920231410135.jpg "Western Blot: p38 alpha [p Tyr323] Antibody [NBP3-23249] - A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38 alpha (a.a. 319-328) (lanes 1 & 2) or anti-p38 alpha (Tyr-323) (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: p38 alpha [p Tyr323] Antibody [NBP3-23249] - Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38 alpha MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23249_rabbit-p38-alpha-p-tyr323-pab-19920231911472.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] - Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI 3-Kinase p85 alpha (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).](http://images.novusbio.com/fullsize/nbp3-23296_mouse-pi-3-kinase-p85-alpha-mab-m253-199202312523621.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] - Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI 3-Kinase p85 alpha (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).")
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p85 alpha Antibody (M253) [NBP3-23296] - Immunocytochemical labeling of PI 3-Kinase p85 alpha in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal PI 3-Kinase p85 alpha (C-terminal region) antibody (NBP3-23296), then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23296_mouse-pi-3-kinase-p85-alpha-mab-m253-199202318202914.jpg)
![Western Blot: MARK2 Antibody [NBP1-71890] - Whole cell lysate from 293T (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-MARK2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. MARK2 was also immunoprecipitated by rabbit anti-MARK2 antibody NBP1-71889, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/MARK2-Antibody-Western-Blot-NBP1-71890-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: MARK2 Antibody [NBP1-71890] - Whole cell lysate from 293T (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-MARK2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. MARK2 was also immunoprecipitated by rabbit anti-MARK2 antibody NBP1-71889, which recognizes an upstream epitope.")
![Western Blot: STK40 Antibody [NBP1-33391] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with serine/threonine kinase 40 antibody [N3C3] diluted at 1:5000.](http://images.novusbio.com/fullsize/STK40-Antibody-Western-Blot-NBP1-33391-img0006.jpg "Western Blot: STK40 Antibody [NBP1-33391] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with serine/threonine kinase 40 antibody [N3C3] diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: STK40 Antibody [NBP1-33391] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/STK40-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33391-img0003.jpg)
![Western Blot: STK40 Antibody [NBP1-33391] - Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 10% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/STK40-Antibody-Western-Blot-NBP1-33391-img0004.jpg)
![Western Blot: MST3 Antibody [NBP1-31575] - Sample(30ug whole cell lysate)A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MST3-Antibody-Western-Blot-NBP1-31575-img0003.jpg "Western Blot: MST3 Antibody [NBP1-31575] - Sample(30ug whole cell lysate)A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 10% SDS PAGE, antibody diluted at 1:1000.")
![STK24 antibody [N1C1] detects STK24 protein at cytosol on HSC-3 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HSC-3 xenograft. STK24 antibody [N1C1] (NBP1-31575) dilution: 1:500. Antigen Retrieval: (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-31575_rabbit-polyclonal-mst3-antibody-immunohistochemistry-141120251830221.jpg)
![Immunoprecipitation: ILK Antibody (6A5) [NBP3-26198] - Immunoprecipitating ILK in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26198 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26198 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26198_rabbit-ilk-mab-6a5-26220241322150.jpg "Immunoprecipitation: ILK Antibody (6A5) [NBP3-26198] - Immunoprecipitating ILK in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26198 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26198 (2ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).")
![Western Blot: ILK Antibody (6A5) [NBP3-26198] - Positive Western Blot detected in: Hela whole cell lysate, HL60 whole cell lysate, A549 whole cell lysate, Mouse kidney tissue All lanes: ILK Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 45, 37 kDa Observed band size: 51 kDa](http://images.novusbio.com/fullsize/nbp3-26198_rabbit-ilk-mab-6a5-28220241152518.jpg)
![Immunocytochemistry/Immunofluorescence: ILK Antibody (6A5) [NBP3-26198] - Staining of MCF7 Cells with ILK Antibody (6A5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26198_rabbit-ilk-mab-6a5-28220241323625.jpg)
![Western Blot: MAP3K9 Antibody [NBP1-33704] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K9 antibody diluted by 1:500.](http://images.novusbio.com/fullsize/MAP3K9-Antibody-Western-Blot-NBP1-33704-img0004.jpg "Western Blot: MAP3K9 Antibody [NBP1-33704] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K9 antibody diluted by 1:500.")
![Immunocytochemistry/Immunofluorescence: MAP3K9 Antibody [NBP1-33704] - Analysis of paraformaldehyde-fixed A549, using MAP3K9 (NBP1-33704) antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MAP3K9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33704-img0002.jpg)
![Western Blot: MAP3K9 Antibody [NBP1-33704] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/MAP3K9-Antibody-Western-Blot-NBP1-33704-img0003.jpg)
![Immunohistochemistry-Paraffin: IRAK1BP1 Antibody [NBP1-31863] - Paraffin-embedded rat intestine. IRAK1BP1 antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/IRAK1BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-31863-img0003.jpg "Immunohistochemistry-Paraffin: IRAK1BP1 Antibody [NBP1-31863] - Paraffin-embedded rat intestine. IRAK1BP1 antibody [N1C3] diluted at 1:500.")
![Immunohistochemistry-Paraffin: IRAK1BP1 Antibody [NBP1-31863] - Paraffin-embedded placenta. IRAK1BP1 antibody [N1C3] dilution: 1:100.](http://images.novusbio.com/fullsize/IRAK1BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-31863-img0001.jpg)
![Immunohistochemistry-Paraffin: IRAK1BP1 Antibody [NBP1-31863] - Paraffin-embedded mouse kidney. IRAK1BP1 antibody [N1C3]) diluted at 1:500.](http://images.novusbio.com/fullsize/IRAK1BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-31863-img0002.jpg)