| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26535-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34643-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26206-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26493-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26247-50ul | |||
![Immunohistochemistry-Paraffin: Casein Kinase 1 delta Antibody (C3) [NBP3-41749] - DAB staining on IHC-P; Sample: Human Cerebrum Tissue; Primary Ab: 30ug/ml NBP3-41749; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41749_mouse-casein-kinase-1-delta-mab-c3-6122024212756.jpg "Immunohistochemistry-Paraffin: Casein Kinase 1 delta Antibody (C3) [NBP3-41749] - DAB staining on IHC-P; Sample: Human Cerebrum Tissue; Primary Ab: 30ug/ml NBP3-41749; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: Casein Kinase 1 delta Antibody (C3) [NBP3-41749] - Sample: Lane1: 293T cell lysate; Lane2: Porcine Cerebrum lysate Primary Ab: 1.5ug/ml NBP3-41749; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41749_mouse-casein-kinase-1-delta-mab-c3-61220242116272.jpg)
![Western Blot: Casein Kinase 1 delta Antibody (C3) [NBP3-41749] - Sample: Lane2: Rat Cerebrum lysate; Lane2: Human Hela cells lysate; Lane3: Human K562 cells lysate; Primary Ab: 3ug/ml NBP3-41749; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41749_mouse-casein-kinase-1-delta-mab-c3-612202421373072.jpg)
![Western Blot: SMCHD1 Antibody [NBP1-49968] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Western-Blot-NBP1-49968-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMCHD1 Antibody [NBP1-49968] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-49968] - Sample: FFPE section of human testicular seminoma (left) and non-small cell lung cancer (right). Antibody: Affinity purified rabbit anti- SmcHD1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Immunohistochemistry-NBP1-49968-img0004.jpg)
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-2622024151064.jpg "Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.")
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-272202485630.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Staining of A549 with HIST1H3A [ac Lys18] Antibody (30A7) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 490-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202412493638.jpg)
![Flow Cytometry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Overlay Peak curve showing A549 cells stained with HIST1H3A [ac Lys18] Antibody (30A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202414312785.jpg)
![Western Blot: STK40 Antibody [NBP2-20519] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with STK40 antibody [N1N3] (NBP2-20519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20519_rabbit-polyclonal-stk40-antibody-910202420122082.jpg "Western Blot: STK40 Antibody [NBP2-20519] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with STK40 antibody [N1N3] (NBP2-20519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: STK40 Antibody [NBP2-20519] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/STK40-Antibody-Western-Blot-NBP2-20519-img0001.jpg)
![STK40 antibody [N1N3] detects STK40 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: STK40 protein stained by STK40 antibody [N1N3] (NBP2-20519) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-20519_rabbit-polyclonal-stk40-antibody-immunocytochemistry-immunofluorescence-2611202518281010.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/718) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-718-Immunocytochemistry-Immunofluorescence-NBP2-32864-img0005.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/718) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).")
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Flow Cytometric Analysis of human MCF-7 cells. ErbB2/Her2 Antibody (HRB2/718) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-718-Flow-Cytometry-NBP2-32864-img0003.jpg)
![Protein Array: ErbB2/Her2 Antibody (HRB2/718) [NBP2-32864] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/718) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-718-Protein-Array-NBP2-32864-img0004.jpg)
![Western Blot: PASK Antibody [NBP2-19704] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PASK-Antibody-Western-Blot-NBP2-19704-img0001.jpg "Western Blot: PASK Antibody [NBP2-19704] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 5% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: PASK Antibody [NBP2-19704] - xenograft, using PASK antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PASK-Antibody-Immunohistochemistry-Paraffin-NBP2-19704-img0002.jpg)
![Immunohistochemistry: IKK beta Antibody (3E1) [NBP3-26206] - Image of IKK beta Antibody (3E1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26206_rabbit-ikk-beta-mab-3e1-262202415475613.jpg "Immunohistochemistry: IKK beta Antibody (3E1) [NBP3-26206] - Image of IKK beta Antibody (3E1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: IKK beta Antibody (3E1) [NBP3-26206] - Positive Western Blot detected in: K562 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate. All lanes: IKK beta Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87 kDa Observed band size: 87 kDa](http://images.novusbio.com/fullsize/nbp3-26206_rabbit-ikk-beta-mab-3e1-28220241140932.jpg)
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody (3E1) [NBP3-26206] - Staining of Hela Cells with IKK beta Antibody (3E1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26206_rabbit-ikk-beta-mab-3e1-2822024132367.jpg)
![Western Blot: UCKL1 Antibody [NBP1-42680] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UCKL1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. UCKL1 was also immunoprecipitated by rabbit anti-UCKL1 antibody NBP1-42681, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/UCKL1-Antibody-Western-Blot-NBP1-42680-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: UCKL1 Antibody [NBP1-42680] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-UCKL1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. UCKL1 was also immunoprecipitated by rabbit anti-UCKL1 antibody NBP1-42681, which recognizes a downstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: UCKL1 Antibody [NBP1-42680] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-UCKL1 used at a dilution of 1:200 (1ug/ml) Detection: DAB](http://images.novusbio.com/fullsize/UCKL1-Antibody-Immunohistochemistry-NBP1-42680-img0003.jpg)
![Western Blot: SMCHD1 Antibody [NBP1-49969] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Western-Blot-NBP1-49969-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMCHD1 Antibody [NBP1-49969] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: SMCHD1 Antibody [NBP1-49969] - Sample: FFPE section of human non-small cell lung cancer (left) and testicular seminoma (right). Antibody: Affinity purified rabbit anti- SmcHD1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Immunohistochemistry-NBP1-49969-img0002.jpg)
![Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] - Immunoprecipitating ATM in PC3 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26493 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26493 (3ug) + PC3 whole cell lysate (500ug). Lane 3: PC3 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-262202413221524.jpg "Immunoprecipitation: ATM Antibody (4E11) [NBP3-26493] - Immunoprecipitating ATM in PC3 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26493 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26493 (3ug) + PC3 whole cell lysate (500ug). Lane 3: PC3 whole cell lysate (20ug).")
![Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-26220241547561.jpg)
![Immunohistochemistry: ATM Antibody (4E11) [NBP3-26493] - Image of ATM Antibody (4E11) diluted at 1:205 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-2722024845325.jpg)
![Western Blot: ATM Antibody (4E11) [NBP3-26493] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate. All lanes: ATM Antibody at 2.05ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 350 KDa Observed band size: 350 KDa](http://images.novusbio.com/fullsize/nbp3-26493_rabbit-atm-mab-4e11-28220241140928.jpg)
![Western Blot: EphA10 Antibody [NBP3-41254] - Sample: Rat Testis lysate; Primary Ab: 1ug/ml NBP3-41254; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41254_rabbit-epha10-pab-61220242195729.jpg "Western Blot: EphA10 Antibody [NBP3-41254] - Sample: Rat Testis lysate; Primary Ab: 1ug/ml NBP3-41254; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody")
![<b>Genetic Strategies Validation. </b>Western Blot: EphA10 Antibody [NBP3-41254] - Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: EPHA10 knockout K562 cell lysate; Predicted MW: 32,97,110kd ; Observed MW: 90kd; Primary Ab: 1ug/ml NBP3-41254; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;](http://images.novusbio.com/fullsize/nbp3-41254_rabbit-epha10-pab-612202421395327.jpg)
![Western Blot: EphA10 Antibody [NBP3-41254] - Sample: Human K562 cell lysate; Primary Ab: 1ug/ml NBP3-41254; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41254_rabbit-epha10-pab-6122024212766.jpg)
![Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202411351616.jpg "Western Blot: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Positive Western Blot detected in: U87 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, THP-1 whole cell lysate, A549 whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: 14-3-3 beta/alpha Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 29, 28 kDa Observed band size: 25-35 kDa")
![Immunocytochemistry/Immunofluorescence: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Staining of PC-3 cell with 14-3-3 beta/alpha Antibody (6F6) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 494-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202412493639.jpg)
![Flow Cytometry: 14-3-3 beta/alpha Antibody (6F6) [NBP3-26247] - Overlay Peak curve showing Hela cells stained with 14-3-3 beta/alpha Antibody (6F6) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26247_rabbit-14-3-3-beta-alpha-mab-6f6-282202414183655.jpg)