| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92968-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38589-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37289-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52462-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37312-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96875-0.025mg | |||
![Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts of various cell lines, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Western-Blot-NBP2-92968-img0005.jpg "Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts of various cell lines, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Casein Kinase 1 epsilon Antibody [NBP2-92968] - Analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using Casein Kinase 1 epsilon at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% non](http://images.novusbio.com/fullsize/Casein-Kinase-1-epsilon-Antibody-Knockout-Validated-NBP2-92968-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 1 epsilon Antibody - BSA Free [NBP2-92968] - Immunofluorescence analysis of HeLa cells using [KO Validated] Casein Kinase 1 epsilon Rabbit pAb . Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92968_rabbit-polyclonal-casein-kinase-1-epsilon-antibody-422025143182.jpg%3Ft=1709606037)
![Immunohistochemistry: PERK [p Thr982] Antibody (0A1E6) [NBP3-33575] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PERK Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33575_rabbit-perk-p-thr982-mab-0a1e6-101220241243981.jpg "Immunohistochemistry: PERK [p Thr982] Antibody (0A1E6) [NBP3-33575] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PERK Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: PERK [p Thr982] Antibody (0A1E6) [NBP3-33575] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using PERK Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33575_rabbit-perk-p-thr982-mab-0a1e6-101220241252865.jpg)
![Immunohistochemistry: PERK [p Thr982] Antibody (0A1E6) [NBP3-33575] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using PERK Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33575_rabbit-perk-p-thr982-mab-0a1e6-1012202411545511.jpg)
![Immunocytochemistry/ Immunofluorescence: PASK Antibody [NBP3-38589] - Immunofluorescence analysis of L929 cells using PASK Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38589_rabbit-pask-pab-91220241519510.jpg "Immunocytochemistry/ Immunofluorescence: PASK Antibody [NBP3-38589] - Immunofluorescence analysis of L929 cells using PASK Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: PASK Antibody [NBP3-38589] - Immunohistochemistry analysis of paraffin-embedded Rat testis using PASK Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38589_rabbit-pask-pab-912202415111846.jpg)
![Western Blot: PASK Antibody [NBP3-38589] - Western blot analysis of various lysates using PASK Rabbit pAb at 1:3000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38589_rabbit-pask-pab-912202415121385.jpg)
![Immunocytochemistry/ Immunofluorescence: PASK Antibody [NBP3-38589] - Immunofluorescence analysis of C6 cells using PASK Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38589_rabbit-pask-pab-912202414112876.jpg)
![Immunohistochemistry: PASK Antibody [NBP3-38589] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PASK Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38589_rabbit-pask-pab-91220241581275.jpg)
![Western Blot: Fyn Antibody (2A10) [NBP2-37588] - Western blot analysis using FYN mAb against human FYN (AA: 7-176) recombinant protein. (Expected MW is 44.3 kDa)](http://images.novusbio.com/fullsize/Fyn-Antibody-2A10-Western-Blot-NBP2-37588-img0002.jpg "Western Blot: Fyn Antibody (2A10) [NBP2-37588] - Western blot analysis using FYN mAb against human FYN (AA: 7-176) recombinant protein. (Expected MW is 44.3 kDa)")
![Immunocytochemistry/Immunofluorescence: Fyn Antibody (2A10) [NBP2-37588] - Immunofluorescence analysis of U251 cells using FYN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Fyn-Antibody-2A10-Immunocytochemistry-Immunofluorescence-NBP2-37588-img0003.jpg)
![Immunohistochemistry: Fyn Antibody (2A10) [NBP2-37588] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using FYN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Fyn-Antibody-2A10-Immunohistochemistry-NBP2-37588-img0005.jpg)
![Flow Cytometry: Fyn Antibody (2A10) [NBP2-37588] - Flow cytometric analysis of Hela cells using FYN mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Fyn-Antibody-2A10-Flow-Cytometry-NBP2-37588-img0004.jpg)
![ELISA: Fyn Antibody (2A10) [NBP2-37588] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Fyn-Antibody-2A10-ELISA-NBP2-37588-img0001.jpg)
![Western Blot: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis using CD247 mAb against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Western-Blot-NBP1-42561-img0011.jpg "Western Blot: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis using CD247 mAb against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of K562 cells using anti-CD247 mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Immunocytochemistry-Immunofluorescence-NBP1-42561-img0009.jpg)
![Immunohistochemistry-Paraffin: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of human Thymus tissues using anti-CD247 mouse mAb](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Immunohistochemistry-Paraffin-NBP1-42561-img0010.jpg)
![Flow Cytometry: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of Jurkat cells using anti-CD247 mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/CD3-zeta-Antibody-4B10-Flow-Cytometry-NBP1-42561-img0012.jpg)
![Western Blot: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis using CSF1R mAb against HEK293 (1) and CSF1R (AA: 344-497)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-Western-Blot-NBP2-37289-img0003.jpg "Western Blot: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis using CSF1R mAb against HEK293 (1) and CSF1R (AA: 344-497)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis of prostate cancer tissues using CSF1R mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-Immunohistochemistry-Paraffin-NBP2-37289-img0007.jpg)
![Flow Cytometry: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis of HepG2 cells using CSF1R mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-Flow-Cytometry-NBP2-37289-img0004.jpg)
![Western Blot: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis using CSF1R mAb against human CSF1R (AA: 344-497) recombinant protein. (Expected MW is 43.3 kDa)](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-Western-Blot-NBP2-37289-img0002.jpg)
![Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9H2) [NBP2-37289] - Analysis of cervical cancer tissues using CSF1R mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-Immunohistochemistry-Paraffin-NBP2-37289-img0008.jpg)
![ELISA: M-CSF R/CD115 Antibody (6B9H2) [NBP2-37289] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/M-CSF-R-CD115-Antibody-6B9H2-ELISA-NBP2-37289-img0001.jpg)
![Western Blot: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Analysis using ErbB4/Her4 mAb against human ERBB4 (AA: 1159-1308) recombinant protein. (Expected MW is 43.3 kDa).](http://images.novusbio.com/fullsize/ErbB4-Her4-Antibody-5G6B4-Western-Blot-NBP2-52462-img0008.jpg "Western Blot: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Analysis using ErbB4/Her4 mAb against human ERBB4 (AA: 1159-1308) recombinant protein. (Expected MW is 43.3 kDa).")
![Immunohistochemistry-Paraffin: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Analysis of paraffin-embedded cervical cancer tissues using ERBB4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ErbB4-Her4-Antibody-5G6B4-Immunohistochemistry-Paraffin-NBP2-52462-img0003.jpg)
![Flow Cytometry: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Analysis of Hela cells using ERBB4 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ErbB4-Her4-Antibody-5G6B4-Flow-Cytometry-NBP2-52462-img0002.jpg)
![Western Blot: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Analysis using ERBB4 mAb against HEK293 (1) and ERBB4 -hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ErbB4-Her4-Antibody-5G6B4-Western-Blot-NBP2-52462-img0006.jpg)
![ELISA: ErbB4/Her4 Antibody (5G6B4) [NBP2-52462] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/ErbB4-Her4-Antibody-5G6B4-ELISA-NBP2-52462-img0001.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0004.jpg "Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mouse mAb against Hela (1), HEK293 (2), A431 (3), MCF-7 (4), RAW264.7 (5), Cos7 (6), C6 (7), Jurkat (8) and NIH/3T3 (9) cell lysate.")
![Immunohistochemistry: p38 Antibody (10B11C8) [NBP2-37312] - Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using MAPK14 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Immunohistochemistry-NBP2-37312-img0005.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mAb against human MAPK14 recombinant protein. (Expected MW is 32.6 kDa)](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0002.jpg)
![Western Blot: p38 Antibody (10B11C8) [NBP2-37312] - Western blot analysis using MAPK14 mAb against HEK293 (1) and MAPK14 (AA: 299-360)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-Western-Blot-NBP2-37312-img0003.jpg)
![ELISA: p38 Antibody (10B11C8) [NBP2-37312] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/p38-Antibody-10B11C8-ELISA-NBP2-37312-img0001.jpg)
![Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Western-Blot-NB110-96875-img0008.jpg "Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: p38 alpha Antibody (9F12) [NB110-96875] - Immunohistochemistry analysis using Mouse Anti-p38 alpha Monoclonal Antibody, Clone 9F12 (NB110-96875). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 alpha Monoclonal Antibody (NB110-96875) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Immunohistochemistry-NB110-96875-img0009.jpg)
![Immunocytochemistry: SGK3 Antibody - BSA Free [NB100-59014] - Immunohistochemical analysis of brain tissue, senile plaques caused by Alzheimer's.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunocytochemistry-NB100-59014-img0004.jpg "Immunocytochemistry: SGK3 Antibody - BSA Free [NB100-59014] - Immunohistochemical analysis of brain tissue, senile plaques caused by Alzheimer's.")
![Immunohistochemistry-Paraffin: SGK3 Antibody - BSA Free [NB100-59014] - Anti-SGK3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59014-img0010.jpg)
![Immunohistochemistry-Paraffin: SGK3 Antibody - BSA Free [NB100-59014] - Anti-SGK3 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59014-img0003.jpg)
![Immunohistochemistry: SGK3 Antibody - BSA Free [NB100-59014] - Immunohistochemical analysis of the brain, hypothalamus. Neurons in the adjacent basal nucleus of Meynert](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-NB100-59014-img0005.jpg)
![Immunohistochemistry: SGK3 Antibody - BSA Free [NB100-59014] - Immunohistochemical analysis of brain, substantia nigra.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-NB100-59014-img0006.jpg)
![Immunohistochemistry-Paraffin: SGK3 Antibody - BSA Free [NB100-59014] - Anti-SGK3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59014-img0009.jpg)
![Immunohistochemistry-Paraffin: SGK3 Antibody - BSA Free [NB100-59014] - Anti-SGK3 antibody IHC of human pancreas, islet. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/SGK3-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB100-59014-img0008.jpg)
![Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-298202414333627.jpg "Immunohistochemistry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32228) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Immunocytochemistry analysis of HeLa cells labeling Casein Kinase 2 alpha with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Casein Kinase 2 alpha antibody (NBP3-32228) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-2920241192348.jpg)
![Flow Cytometry: Casein Kinase 2 alpha Antibody (PSH04-54) [NBP3-32228] - Flow cytometric analysis of HeLa cells labeling Casein Kinase 2 alpha.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32228, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32228_rabbit-casein-kinase-2-alpha-mab-psh04-54-29202416453220.jpg)
![Western blot analysis on different lysates with NBP3-32228 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 45 kDa Observed band size: 40 kDa Exposure time: Lane 1-7 (left): 40 seconds; Lane 1-7 (right): 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32228 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32228_-western-blot-639204597488042700.jpg)
![ELISA: Human ROCK2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40359] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40359_human-rock2-ready-to-use-elisa-kit-colorimetric-2110202415305296.png "ELISA: Human ROCK2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40359] - Standard Curve Reference")