| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85759-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26216-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26439-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26542-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26379-50ul | |||
![Immunohistochemistry-Paraffin: CDCP1 Antibody [NBP1-85759] - Analysis in human duodenum and lymph node tissues using NBP1-85759 antibody. Corresponding CDCP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-85759_rabbit-polyclonal-cdcp1-antibody-immunohistochemistry-paraffin-822024134528..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDCP1 Antibody [NBP1-85759] - Analysis in human duodenum and lymph node tissues using NBP1-85759 antibody. Corresponding CDCP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDCP1 Antibody [NBP1-85759] - Staining of human duodenum shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-85759_rabbit-polyclonal-cdcp1-antibody-immunohistochemistry-paraffin-822024134359..jpg)
![Immunohistochemistry-Paraffin: CDCP1 Antibody [NBP1-85759] - Staining of human Fallopian tube shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-85759_rabbit-polyclonal-cdcp1-antibody-immunohistochemistry-paraffin-822024134638..jpg)
![Immunohistochemistry-Paraffin: CDCP1 Antibody [NBP1-85759] -Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-85759_rabbit-polyclonal-cdcp1-antibody-immunohistochemistry-paraffin-822024134758..jpg)
![Western Blot: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4C. For detection Dye light 800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature](http://images.novusbio.com/fullsize/STAT5A-p-Tyr694-Antibody-5F6-F1-Western-Blot-NBP1-77817-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4C. For detection Dye light 800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Used at a 1:500 dilution detecting Stat5 in 3T3 cells (immunofluorescent STED microscopy). Red represents Stat5 pY694 protein. Green represents tubulin.](http://images.novusbio.com/fullsize/STAT5A-p-Tyr694-Antibody-5F6-F1-Immunocytochemistry-Immunofluorescence-NBP1-77817-img0006.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: STAT5A [p Tyr694] Antibody (5F6.F1) [NBP1-77817] - Analysis of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used at a dilution of 1 to 20 ug/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/STAT5A-p-Tyr694-Antibody-5F6-F1-Immunohistochemistry-NBP1-77817-img0005.jpg)
![Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho BRCA1 (S1524) antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1524]-Antibody-Western-Blot-NB100-200-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200] - Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho BRCA1 (S1524) antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: BRCA1 [p Ser1524] Antibody [NB100-200] - Nuclear extract (50 ug) from HeLa cells mock irradiated or irradiated with 10 Gy of ionizing radiation. Antibody used at 0.5 ug/ml.](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1524]-Antibody-Western-Blot-NB100-200-img0002.jpg)
![Proximity Ligation Assay: BRCA1 [p Ser1524] Antibody [NB100-200] - Image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1524]-Antibody-Proximity-Ligation-Assay-NB100-200-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Analysis of phosphorylated ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.](http://images.novusbio.com/fullsize/ASAP1-[p-Tyr782]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77933-img0005.jpg "Immunocytochemistry/Immunofluorescence: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Analysis of phosphorylated ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.")
![Immunohistochemistry: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Used at 20 g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/ASAP1-[p-Tyr782]-Antibody-Immunohistochemistry-NBP1-77933-img0004.jpg)
![Western Blot: Serum Response Factor SRF Antibody [NBP1-71927] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-SRF antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SRF was also immunoprecipitated by rabbit anti-SRF antibody NBP1-71928, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/Serum-Response-Factor-SRF-Antibody-Western-Blot-NBP1-71927-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Serum Response Factor SRF Antibody [NBP1-71927] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-SRF antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SRF was also immunoprecipitated by rabbit anti-SRF antibody NBP1-71928, which recognizes a downstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody [NBP1-71927] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SRF used at a dilution of 1:1,000 (1ug/ml) (left) and used at a dilution of 1:1,000 (1ug/ml) (right) . Detection: DAB](http://images.novusbio.com/fullsize/Serum-Response-Factor-SRF-Antibody-Immunohistochemistry-Paraffin-NBP1-71927-img0002.jpg)
![Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20--p-Ser24]-Antibody-Western-Blot-NBP1-78102-img0001.jpg "Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.")
![Immunohistochemistry: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - Used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20-p-Ser24]-Antibody-Immunohistochemistry-NBP1-78102-img0003.jpg)
![Immunohistochemistry-Paraffin: SCL/Tal1 Antibody [NBP1-33757] - Mouse spleen. TAL1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-Immunohistochemistry-Paraffin-NBP1-33757-img0005.jpg "Immunohistochemistry-Paraffin: SCL/Tal1 Antibody [NBP1-33757] - Mouse spleen. TAL1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: SCL/Tal1 Antibody [NBP1-33757] - A549 Xenograft , using TAL1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-Immunohistochemistry-Paraffin-NBP1-33757-img0007.jpg)
![Immunoprecipitation: SCL/Tal1 Antibody [NBP1-33757] - IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TAL1 protein by 2 ug of TAL1 antibody 12% SDS-PAGE The immunoprecipitated TAL1 protein was detected by TAL1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-Immunoprecipitation-NBP1-33757-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SCL/Tal1 Antibody [NBP1-33757] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TAL1 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33757_rabbit-polyclonal-scl-tal1-antibody-1610202419414533.jpg)
![Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-262202415355731.jpg "Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: HSP90 beta Antibody (7G7) [NBP3-26216] - Image of HSP90 beta Antibody (7G7) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-26220241522415.jpg)
![Western Blot: HSP90 beta Antibody (7G7) [NBP3-26216] - Positive Western Blot detected in: HepG2 whole cell lysate, U251 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate.<br /> All lanes: HSP90 beta Antibody at 1: 2000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 84 kDa<br /> Observed band size: 80-90 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-282202411485820.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (7G7) [NBP3-26216] - Staining of HepG2 cell with HSP90 beta Antibody (7G7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 495-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-28220241312214.jpg)
![Flow Cytometry: HSP90 beta Antibody (7G7) [NBP3-26216] - Overlay Peak curve showing Hela cells stained with HSP90 beta Antibody (7G7) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26216_rabbit-hsp90-beta-mab-7g7-282202414312789.jpg)
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-262202415475644.jpg "Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-2722024850467.jpg)
![Western Blot: PARP Antibody (8C7) [NBP3-26439] - Positive Western Blot detected in: K562 whole cell lysate.<br /> All lanes: PARP Antibody at 1: 2000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 114 KDa<br /> Observed band size: 114 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-282202411485824.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (8C7) [NBP3-26439] - Staining of Hela Cells with PARP Antibody (8C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241323611.jpg)
![Flow Cytometry: PARP Antibody (8C7) [NBP3-26439] - Overlay histogram showing Jurkat cells stained with PARP Antibody (8C7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241431270.jpg)
![Immunohistochemistry: GSK-3 beta Antibody (9G11) [NBP3-26542] - Image of GSK-3 beta Antibody (9G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26542_rabbit-gsk-3-beta-mab-9g11-262202414471519.jpg "Immunohistochemistry: GSK-3 beta Antibody (9G11) [NBP3-26542] - Image of GSK-3 beta Antibody (9G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: GSK-3 beta Antibody (9G11) [NBP3-26542] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate.<br /> All lanes: GSK3 beta Antibody at 1: 1000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 47, 49 kDa<br /> Observed band size: 47 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26542_rabbit-gsk-3-beta-mab-9g11-28220241140910.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (9G11) [NBP3-26542] - Staining of Hela Cells with GSK-3 beta Antibody (9G11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26542_rabbit-gsk-3-beta-mab-9g11-282202412475130.jpg)
![Immunohistochemistry: PKN2 Antibody (20A8) [NBP3-26379] - Image of PKN2 Antibody (20A8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26379_rabbit-pkn2-mab-20a8-2722024856311.jpg "Immunohistochemistry: PKN2 Antibody (20A8) [NBP3-26379] - Image of PKN2 Antibody (20A8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: PKN2 Antibody (20A8) [NBP3-26379] - Positive Western Blot detected in: Hela whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate.<br /> All lanes: PKN2 Antibody at 1: 2000<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 113, 117, 112 kDa<br /> Observed band size: 130 kDa<br />](http://images.novusbio.com/fullsize/nbp3-26379_rabbit-pkn2-mab-20a8-282202411442639.jpg)
![Immunocytochemistry/Immunofluorescence: PKN2 Antibody (20A8) [NBP3-26379] - Staining of MCF-7 cell with PKN2 Antibody (20A8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 509-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26379_rabbit-pkn2-mab-20a8-28220241247510.jpg)
![Flow Cytometry: PKN2 Antibody (20A8) [NBP3-26379] - Overlay Peak curve showing MCF7 cells stained with PKN2 Antibody (20A8) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26379_rabbit-pkn2-mab-20a8-282202414183661.jpg)