| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85759-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26228-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26439-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26680-50ul | |||
![Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444] - Western blot analysis of HSF1 on different lysates with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lane 4: HEK-293 cell lysate Lane 5: K-562 cell lysate Lane 6: Jurkat cell lysate Lane 7: A549 cell lysate Lane 8: HepG2 cell lysate Lane 9: LoVo cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-278202415242677.jpg "Western Blot: HSF1 Antibody (JE44-79) [NBP3-32444] - Western blot analysis of HSF1 on different lysates with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: U-2 OS cell lysate Lane 3: MCF7 cell lysate Lane 4: HEK-293 cell lysate Lane 5: K-562 cell lysate Lane 6: Jurkat cell lysate Lane 7: A549 cell lysate Lane 8: HepG2 cell lysate Lane 9: LoVo cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32444) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-29820241420917.jpg)
![Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (JE44-79) [NBP3-32444] - Immunocytochemistry analysis of HeLa cells labeling HSF1 with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-HSF1 antibody (NBP3-32444) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-2920241033401.jpg)
![Flow Cytometry: HSF1 Antibody (JE44-79) [NBP3-32444] - Flow cytometric analysis of HeLa cells labeling HSF1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32444, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32444_rabbit-hsf1-mab-je44-79-392024884655.jpg)
![Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-278202416331255.jpg "Western Blot: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Western blot analysis of p21/CIP1/CDKN1A on different lysates with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 18 kDa Observed band size: 21 kDa Exposure time: Lane 1: 1 minute; Lane 2: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32691) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29820241426265.jpg)
![Immunocytochemistry/ Immunofluorescence: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Immunocytochemistry analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A with Rabbit anti-p21/CIP1/CDKN1A/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p21/CIP1/CDKN1A antibody (NBP3-32691) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202410334083.jpg)
![Flow Cytometry: p21/CIP1/CDKN1A Antibody (PSH03-93) [NBP3-32691] - Flow cytometric analysis of RAW264.7 cells labeling p21/CIP1/CDKN1A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32691, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32691_rabbit-p21-cip1-cdkn1a-mab-psh03-93-29202416372178.jpg)
![Western Blot: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Western blot analysis of IL-7R alpha/CD127 on K562 cell lysates with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32122) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-278202415242671.jpg "Western Blot: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Western blot analysis of IL-7R alpha/CD127 on K562 cell lysates with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 52 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32122) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32122) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-29820241492652.jpg)
![Immunocytochemistry/ Immunofluorescence: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Immunocytochemistry analysis of SiHa cells labeling IL-7R alpha/CD127 with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL-7R alpha/CD127 antibody (NBP3-32122) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-29202410484826.jpg)
![Flow Cytometry: IL-7R alpha/CD127 Antibody (JE37-10) [NBP3-32122] - Flow cytometric analysis of K-562 cells labeling IL-7R alpha/CD127. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32122, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32122_rabbit-il-7r-alpha-cd127-mab-je37-10-392024884614.jpg)
![Western Blot: Serum Response Factor SRF Antibody [NBP1-71927] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-SRF antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SRF was also immunoprecipitated by rabbit anti-SRF antibody NBP1-71928, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/Serum-Response-Factor-SRF-Antibody-Western-Blot-NBP1-71927-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Serum Response Factor SRF Antibody [NBP1-71927] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 mcg) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-SRF antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. SRF was also immunoprecipitated by rabbit anti-SRF antibody NBP1-71928, which recognizes a downstream epitope.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Serum Response Factor SRF Antibody [NBP1-71927] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SRF used at a dilution of 1:1,000 (1ug/ml) (left) and used at a dilution of 1:1,000 (1ug/ml) (right) . Detection: DAB](http://images.novusbio.com/fullsize/Serum-Response-Factor-SRF-Antibody-Immunohistochemistry-Paraffin-NBP1-71927-img0002.jpg)
![Immunohistochemistry-Paraffin: CDCP1 Antibody [NBP1-85759] - Analysis in human duodenum and lymph node tissues using NBP1-85759 antibody. Corresponding CDCP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp1-85759_rabbit-polyclonal-cdcp1-antibody-immunohistochemistry-paraffin-822024134528..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDCP1 Antibody [NBP1-85759] - Analysis in human duodenum and lymph node tissues using NBP1-85759 antibody. Corresponding CDCP1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CDCP1 Antibody [NBP1-85759] - Staining of human duodenum shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-85759_rabbit-polyclonal-cdcp1-antibody-immunohistochemistry-paraffin-822024134359..jpg)
![Immunohistochemistry-Paraffin: CDCP1 Antibody [NBP1-85759] - Staining of human Fallopian tube shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-85759_rabbit-polyclonal-cdcp1-antibody-immunohistochemistry-paraffin-822024134638..jpg)
![Immunohistochemistry-Paraffin: CDCP1 Antibody [NBP1-85759] -Staining of human prostate shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp1-85759_rabbit-polyclonal-cdcp1-antibody-immunohistochemistry-paraffin-822024134758..jpg)
![Immunohistochemistry-Paraffin: SCL/Tal1 Antibody [NBP1-33757] - Mouse spleen. TAL1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-Immunohistochemistry-Paraffin-NBP1-33757-img0005.jpg "Immunohistochemistry-Paraffin: SCL/Tal1 Antibody [NBP1-33757] - Mouse spleen. TAL1 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: SCL/Tal1 Antibody [NBP1-33757] - A549 Xenograft , using TAL1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-Immunohistochemistry-Paraffin-NBP1-33757-img0007.jpg)
![Immunoprecipitation: SCL/Tal1 Antibody [NBP1-33757] - IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of TAL1 protein by 2 ug of TAL1 antibody 12% SDS-PAGE The immunoprecipitated TAL1 protein was detected by TAL1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/SCL-Tal1-Antibody-Immunoprecipitation-NBP1-33757-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SCL/Tal1 Antibody [NBP1-33757] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TAL1 antibody [C2C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33757_rabbit-polyclonal-scl-tal1-antibody-1610202419414533.jpg)
![Immunocytochemistry/Immunofluorescence: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Analysis of phosphorylated ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.](http://images.novusbio.com/fullsize/ASAP1-[p-Tyr782]-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77933-img0005.jpg "Immunocytochemistry/Immunofluorescence: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Analysis of phosphorylated ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.")
![Immunohistochemistry: ASAP1 [p Tyr782] Antibody [NBP1-77933] - Used at 20 g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.](http://images.novusbio.com/fullsize/ASAP1-[p-Tyr782]-Antibody-Immunohistochemistry-NBP1-77933-img0004.jpg)
![Immunohistochemistry: Histone H3.1 Antibody (27F2) [NBP3-26228] - Image of Histone H3.1 Antibody (27F2) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26228_rabbit-histone-h3-1-mab-27f2-2722024857331.jpg "Immunohistochemistry: Histone H3.1 Antibody (27F2) [NBP3-26228] - Image of Histone H3.1 Antibody (27F2) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: Histone H3.1 Antibody (27F2) [NBP3-26228] - Image of Histone H3.1 Antibody (27F2) diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26228_rabbit-histone-h3-1-mab-27f2-27220248471915.jpg)
![Western Blot: Histone H3.1 Antibody (27F2) [NBP3-26228] - Positive Western Blot detected in Jurkat whole cell lysate. All lanes: Histone H3.1 Antibody at 1.5ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa Observed band size: 15 KDa](http://images.novusbio.com/fullsize/nbp3-26228_rabbit-histone-h3-1-mab-27f2-282202411485848.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3.1 Antibody (27F2) [NBP3-26228] - Staining of Hela cells with Histone H3.1 Antibody (27F2) at 1:93, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26228_rabbit-histone-h3-1-mab-27f2-2822024132362.jpg)
![Flow Cytometry: Histone H3.1 Antibody (27F2) [NBP3-26228] - Overlay histogram showing Hela cells stained with Histone H3.1 Antibody (27F2) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26228_rabbit-histone-h3-1-mab-27f2-282202414183685.jpg)
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-262202415475644.jpg "Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: PARP Antibody (8C7) [NBP3-26439] - Image of PARP Antibody (8C7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-2722024850467.jpg)
![Western Blot: PARP Antibody (8C7) [NBP3-26439] - Positive Western Blot detected in: K562 whole cell lysate. All lanes: PARP Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 114 KDa Observed band size: 114 kDa](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-282202411485824.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (8C7) [NBP3-26439] - Staining of Hela Cells with PARP Antibody (8C7) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241323611.jpg)
![Flow Cytometry: PARP Antibody (8C7) [NBP3-26439] - Overlay histogram showing Jurkat cells stained with PARP Antibody (8C7) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26439_rabbit-parp-mab-8c7-28220241431270.jpg)
![Western Blot: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate (treated with EGF or Pervanadate). All lanes: LAT antibody at 2.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa.](http://images.novusbio.com/fullsize/LAT-p-Tyr191-Antibody-2E4-Western-Blot-NBP3-15162-img0005.jpg "Western Blot: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - Positive WB detected in: Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate (treated with EGF or Pervanadate). All lanes: LAT antibody at 2.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 38 KDa. Observed band size: 38 KDa.")
![Immunocytochemistry/Immunofluorescence: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - Immunofluorescence staining of Hela cells with at LAT antibody 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/LAT-p-Tyr191-Antibody-2E4-Immunocytochemistry-Immunofluorescence-NBP3-15162-img0001.jpg)
![Immunohistochemistry: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - IHC image of LAT antibody diluted at 1:100 and staining in paraffin-embedded human colon cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/LAT-p-Tyr191-Antibody-2E4-Immunohistochemistry-NBP3-15162-img0004.jpg)
![Immunohistochemistry: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - IHC image of LAT antibody diluted at 1:100 and staining in paraffin-embedded human tonsil tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/LAT-p-Tyr191-Antibody-2E4-Immunohistochemistry-NBP3-15162-img0003.jpg)
![Immunoprecipitation: LAT [p Tyr191] Antibody (2E4) [NBP3-15162] - Immunoprecipitating LAT in A549 whole cell lysate. Lane 1: Rabbit control IgG (1ug) instead of LAT antibody in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: LAT antibody (3ug) + A549 whole cell lysate (1mg). Lane 3: A549 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/LAT-p-Tyr191-Antibody-2E4-Immunoprecipitation-NBP3-15162-img0002.jpg)
![Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-2622024152247.jpg "Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.")
![Immunohistochemistry: CD44 Antibody (19H12) [NBP3-26680] - Image of CD44 Antibody (19H12) diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-262202415253312.jpg)
![Western Blot: CD44 Antibody (19H12) [NBP3-26680] - Positive Western Blot detected in: A549 whole cell lysate, MCF7 whole cell lysate, HepG2 whole cell lysate. All lanes: CD44 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 81 kDa Observed band size: 81 kDa](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-28220241136303.jpg)
![Immunocytochemistry/Immunofluorescence: CD44 Antibody (19H12) [NBP3-26680] - Staining of Hela with CD44 Antibody (19H12) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 515-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-28220241323630.jpg)
![Flow Cytometry: CD44 Antibody (19H12) [NBP3-26680] - Overlay Peak curve showing Hela cells stained with CD44 Antibody (19H12) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26680_rabbit-cd44-mab-19h12-282202414312756.jpg)
![Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20--p-Ser24]-Antibody-Western-Blot-NBP1-78102-img0001.jpg "Western Blot: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 delta20E, delta24E, non-phosphorylated, 4 - HMGN1, delta20E, delta24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1:1,000 for this experiment. The blot was processed using a 5 sec exposure time.")
![Immunohistochemistry: HMGN1/HMG14 [p Ser20, p Ser24] Antibody [NBP1-78102] - Used at 20 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/HMGN1-HMG14-[p-Ser20-p-Ser24]-Antibody-Immunohistochemistry-NBP1-78102-img0003.jpg)
