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![Immunoprecipitation:ATG13 AntibodyNBP3-30274] -Detection of human ATG13 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATG13 antibody NBP3-30274 (lot NBP3-30274-1) used for IP at 6 µg per reaction. ATG13 was also immunoprecipitated by rabbit anti-ATG13 antibody BL20881. For blotting immunoprecipitated ATG13, NBP3-30274 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30274_rabbit-atg13-pab-301220241229398.jpg "Immunoprecipitation:ATG13 AntibodyNBP3-30274] -Detection of human ATG13 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ATG13 antibody NBP3-30274 (lot NBP3-30274-1) used for IP at 6 µg per reaction. ATG13 was also immunoprecipitated by rabbit anti-ATG13 antibody BL20881. For blotting immunoprecipitated ATG13, NBP3-30274 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot:[NBP3-29192]-Detection of human and mouse CDC37 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CDC37 antibody used for WB at 0. 4 µg/ml and 1 µg/ml and used for IP at 3 µg/mg lysate. CDC37 was also immunoprecipitated by rabbit anti-CDC37 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.](http://images.novusbio.com/fullsize/nbp3-29192_rabbit-cdc37-pab-231202512101677.jpg "Western Blot:[NBP3-29192]-Detection of human and mouse CDC37 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CDC37 antibody used for WB at 0. 4 µg/ml and 1 µg/ml and used for IP at 3 µg/mg lysate. CDC37 was also immunoprecipitated by rabbit anti-CDC37 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 10 seconds.")
![Western Blot: RB1 [p Ser780] Antibody [NBP3-13308] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RB1 (phospho Ser780) antibody (NBP3-13308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RB1-[p-Ser780]-Antibody-Western-Blot-NBP3-13308-img0001.jpg "Western Blot: RB1 [p Ser780] Antibody [NBP3-13308] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RB1 (phospho Ser780) antibody (NBP3-13308) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1139) antibody (NBP3-12918) diluted at 1:10000.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1139]-Antibody-Western-Blot-NBP3-12918-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1139) antibody (NBP3-12918) diluted at 1:10000.")
![<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - ErbB2 (phospho Tyr1139) antibody detects ErbB2 (phospho Tyr1139) protein by western blot analysis. Un-treated (-) and treated (+, 200ng/ml Trichostatin A treatment for 15min) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2 (phospho Tyr1139) antibody (NBP3-12918) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1139]-Antibody-Western-Blot-NBP3-12918-img0001.jpg)
![Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.](http://images.novusbio.com/fullsize/ERK2-Knockout-A549-Cell-Lysate-Western-Blot-NBP3-18632-img0001.jpg "Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.")
![Immunoprecipitation:EZH2/KMT6 AntibodyNBP3-30183] -Detection of human EZH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EZH2 antibody (NBP3-30183 lot 2) used for IP at 6 µg per reaction. EZH2 was also immunoprecipitated by a previous lot of this antibody (NBP3-30183 lot 1) and a second.inst a different epitope of EZH2 (A302-196A). For blotting immunoprecipitated EZH2, NBP3-30183 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30183_rabbit-ezh2-kmt6-pab-30122024138248.jpg "Immunoprecipitation:EZH2/KMT6 AntibodyNBP3-30183] -Detection of human EZH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EZH2 antibody (NBP3-30183 lot 2) used for IP at 6 µg per reaction. EZH2 was also immunoprecipitated by a previous lot of this antibody (NBP3-30183 lot 1) and a second.inst a different epitope of EZH2 (A302-196A). For blotting immunoprecipitated EZH2, NBP3-30183 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry:[NBP3-29326]-Detection of human Phospho SMC1 by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Mock treatment or lambda phosphatase-treatment. Antibody: Affinity purified rabbit anti-Phospho-SMC1.](http://images.novusbio.com/fullsize/nbp3-29326_rabbit-smc1-pab-2312025129512.jpg "Immunohistochemistry:[NBP3-29326]-Detection of human Phospho SMC1 by immunohistochemistry. Samples: FFPE sections of human prostate carcinoma. Mock treatment or lambda phosphatase-treatment. Antibody: Affinity purified rabbit anti-Phospho-SMC1.")
![Western Blot:[NBP3-29063]-Detection of human and mouse NPM1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody used for WB at 0. 04 µg/ml and 0. 1 µg/ml and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29063_rabbit-npm1-pab-23120251204028.jpg "Western Blot:[NBP3-29063]-Detection of human and mouse NPM1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody used for WB at 0. 04 µg/ml and 0. 1 µg/ml and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.")
![Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-western-blot-2942025165210..jpg "Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Rabbit Tau [p Thr217] mAb (HL1276) [NBP3-45156] - Indirect ELISA analysis was performed by coating the plate with Tau peptide and Tau (p Thr217) peptide (1000-15.63 ng/mL). Coated peptides were probed with Tau (p Thr217) antibody [HL1276] (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-elisa-2942025164816..jpg)
