| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-29680-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21519-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-81871-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22076-50ul | |||
![Western Blot: DNA-PKcs [p Thr2609] Antibody [NB600-897] - Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNAPK inhibitor + IR, Lane 4: MO59J (DNAPK-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNAPK inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.](http://images.novusbio.com/fullsize/DNA-PKcs-[p-Thr2609]-Antibody-Western-Blot-NB600-897-img0005.jpg "Western Blot: DNA-PKcs [p Thr2609] Antibody [NB600-897] - Lane 1: Fus1 untreated, Lane 2: Fus1 IR (20Gy, 4h), Lane 3: Fus1 DNAPK inhibitor + IR, Lane 4: MO59J (DNAPK-) untreated, Lane 5: MO59J IR, Lane 6: Fus1 untreated, Lane 7: Fus1 IR (20Gy, 4h), Lane 8: Fus1 DNAPK inhibitor + IR, Lane 9: MO59J untreated, Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.")
![Western Blot results of Rabbit DNA-PKcs [p Thr2609] Antibody. Lane 1: Molecular Weight Ladder. Lane 2: DNA Pkc phosphor control. Lane 3: DNA Pkc control. Lane 4: BSA. Load: 10ug. Primary Antibody: Rabbit Anti-DNA Pkcs pT609 at 1ug/mL overnight at 4C. Secondary Antibody: Goat anti-Rabbit HRP](http://images.novusbio.com/fullsize/nb600-897_rabbit-polyclonal-dna-pkcs-p-thr2609-antibody-25520231328193.jpg)
![Western Blot: CDC16 [p Thr580] Antibody [NB600-889] - Analysis of a band 72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.](http://images.novusbio.com/fullsize/CDC16-[p-Thr580]-Antibody-Western-Blot-NB600-889-img0004.jpg "Western Blot: CDC16 [p Thr580] Antibody [NB600-889] - Analysis of a band 72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.")
![Immunohistochemistry-Paraffin: LRP-6 Antibody (C4) [NBP3-41918] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 20ug/ml NBP3-41918; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41918_mouse-lrp-6-mab-c4-612202421373021.jpg "Immunohistochemistry-Paraffin: LRP-6 Antibody (C4) [NBP3-41918] - DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 20ug/ml NBP3-41918; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: LRP-6 Antibody (C4) [NBP3-41918] - DAB staining on IHC-P; Sample: Human Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-41918; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41918_mouse-lrp-6-mab-c4-61220242055489.jpg)
![Western Blot: Rad9 [p Ser1260] Antibody [NBP1-77981] - Affinity purified Rad9 [p Ser1260] antibody used at a 1:200 dilution incubated overnight at 4C to detect Rad9 by Western blot. Lanes loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 by treatment with ATP & Rad53 kinase. Rad53 kinase autophosphorylates & appears cross reactive as it is detected on the blot as 90 & 110 kDa bands (asterisk). Detection occurred using a 1:5,000 IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan & process the image.](http://images.novusbio.com/fullsize/Rad9-[p-Ser1260]-Antibody-Western-Blot-NBP1-77981-img0003.jpg "Western Blot: Rad9 [p Ser1260] Antibody [NBP1-77981] - Affinity purified Rad9 [p Ser1260] antibody used at a 1:200 dilution incubated overnight at 4C to detect Rad9 by Western blot. Lanes loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 by treatment with ATP & Rad53 kinase. Rad53 kinase autophosphorylates & appears cross reactive as it is detected on the blot as 90 & 110 kDa bands (asterisk). Detection occurred using a 1:5,000 IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan & process the image.")
![N/A: Rad9 [p Ser1260] Antibody [NBP1-77981]](http://images.novusbio.com/fullsize/Rad9-[p-Ser1260]-Antibody-N-A-NBP1-77981-img0002.jpg)
![Western Blot: STAT5A [Asym-dimethyl Arg31] Antibody [NBP2-44185] - Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide. Load: 100, 10, or 1 picomolar as indicated.](http://images.novusbio.com/fullsize/STAT5A-[Asym-dimethyl-Arg31]-Antibody-Western-Blot-NBP2-44185-img0001.jpg "Western Blot: STAT5A [Asym-dimethyl Arg31] Antibody [NBP2-44185] - Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide. Load: 100, 10, or 1 picomolar as indicated.")
![Dot blot of Rabbit STAT5A [Asym-dimethyl Arg31] Antibody - BSA Free. Antigen: non-modified, monomethylated and asymmetric dimethylated forms of the immunizing peptide. Load: 100, 10, or 1 picomolar as indicated. Primary antibody: STAT5A [Asym-dimethyl Arg31] Antibody - BSA Free at 1:1000 for 90 min at RT. (Date: 11/04/14; Exposure time: 82 seconds)](http://images.novusbio.com/fullsize/nbp2-44185_rabbit-polyclonal-stat5a-asym-dimethyl-arg31-antibody-187202581879.jpg)
![Western Blot: Myosin Phosphatase [p Thr853] Antibody (SA19) [NBP2-29680] - Untreated HEK293 cell lysate (lane 1) and HEK293 cell lysate phosphorylated by ROKalpha /ROCKII (lane 2) were probed with anti-phospho-MyPT1 (Thr850), clone SA19 (1:1000 dilution).](http://images.novusbio.com/fullsize/Myosin-Phosphatase-p-Thr853-Antibody-SA19-Western-Blot-NBP2-29680-img0001.jpg "Western Blot: Myosin Phosphatase [p Thr853] Antibody (SA19) [NBP2-29680] - Untreated HEK293 cell lysate (lane 1) and HEK293 cell lysate phosphorylated by ROKalpha /ROCKII (lane 2) were probed with anti-phospho-MyPT1 (Thr850), clone SA19 (1:1000 dilution).")
![Immunohistochemistry-Paraffin: LBP Antibody (C1) [NBP3-41480] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 10ug/ml NBP3-41480; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41480_mouse-lbp-mab-c1-612202421243262.jpg "Immunohistochemistry-Paraffin: LBP Antibody (C1) [NBP3-41480] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 10ug/ml NBP3-41480; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: LBP Antibody (C2) [NBP3-41601] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 10ug/ml NBP3-41601; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41601_mouse-lbp-mab-c2-61220242137303.jpg "Immunohistochemistry-Paraffin: LBP Antibody (C2) [NBP3-41601] - DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 10ug/ml NBP3-41601; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: LRRK2 [p Ser 935] Antibody (SR1453) [NBP3-21519] - Western blot analysis using NBP3-21519 in WT-LRRK2 cell lysate treated LRRK2.](http://images.novusbio.com/fullsize/nbp3-21519_rabbit-lrrk2-p-ser-935-mab-sr1453-572023152611.jpg "Western Blot: LRRK2 [p Ser 935] Antibody (SR1453) [NBP3-21519] - Western blot analysis using NBP3-21519 in WT-LRRK2 cell lysate treated LRRK2.")
![Western Blot: Cortactin [p Tyr466] Antibody [NB100-81871] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using Cortactin(Phospho-Tyr466) antibody](http://images.novusbio.com/fullsize/Cortactin-[p-Tyr466]-Antibody-Western-Blot-NB100-81871-img0001.jpg "Western Blot: Cortactin [p Tyr466] Antibody [NB100-81871] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using Cortactin(Phospho-Tyr466) antibody")
![Western Blot: Cortactin [p Tyr466] Antibody [NB100-81871] - Analysis of extracts from JK cells, treated with H2O2 or calf intestinal phosphatase (CIP), using Cortactin (Phospho-Tyr466) Antibody.](http://images.novusbio.com/fullsize/Cortactin-[p-Tyr466]-Antibody-Western-Blot-NB100-81871-img0003.jpg)
![Western Blot: TrkB Antibody (SR2271) [NBP3-22076] - Western blot analysis using NBP3-22076 in mouse brain lysate.](http://images.novusbio.com/fullsize/nbp3-22076_rabbit-trkb-mab-sr2271-5720231531213.jpg "Western Blot: TrkB Antibody (SR2271) [NBP3-22076] - Western blot analysis using NBP3-22076 in mouse brain lysate.")