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![Western Blot: Chk1 Antibody [NBP3-30982] - Western analysis of Chk1 protein in the indicated cell lines. Sheep polyclonal antibody was used at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-30982_sheep-chk1-pab-811202420592127.jpg "Western Blot: Chk1 Antibody [NBP3-30982] - Western analysis of Chk1 protein in the indicated cell lines. Sheep polyclonal antibody was used at 1:500 dilution.")
![Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32058) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: Jurkat cell lysateLane 3: 293T cell lysateLane 4: MCF7 cell lysateLane 5: K-562 cell lysateLane 6: COS-1 cell lysateLane 7: NIH/3T3 cell lysateLane 8: C2C12 cell lysateLane 9: PC-12 cell lysateLane 10: Mouse brain tissue lysateLane 11: Rat brain tissue lysateLane 12: Mouse kidney tissue lysateLane 13: Mouse colon tissue lysateLysates/proteins at 30 µg/Lane.Predicted band size: 44 kDaObserved band size: 47 kDaExposure time: 42 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32058_rabbit-atg4b-mab-psh02-53-3920248453542.jpg "Western Blot: ATG4B Antibody (PSH02-53) [NBP3-32058] - Western blot analysis of ATG4B on different lysates with Rabbit anti-ATG4B antibody (NBP3-32058) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: Jurkat cell lysateLane 3: 293T cell lysateLane 4: MCF7 cell lysateLane 5: K-562 cell lysateLane 6: COS-1 cell lysateLane 7: NIH/3T3 cell lysateLane 8: C2C12 cell lysateLane 9: PC-12 cell lysateLane 10: Mouse brain tissue lysateLane 11: Rat brain tissue lysateLane 12: Mouse kidney tissue lysateLane 13: Mouse colon tissue lysateLysates/proteins at 30 µg/Lane.Predicted band size: 44 kDaObserved band size: 47 kDaExposure time: 42 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: p38 alpha Antibody [NBP3-23232] - Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38 alpha (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38 alpha (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23232_rabbit-p38-alpha-pab-199202312403713.jpg "Western Blot: p38 alpha Antibody [NBP3-23232] - Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38 alpha (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38 alpha (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).")
![Western Blot: Catenin alpha 1 [p Tyr148] Antibody [NBP3-23196] - Western blot analysis of rat PC12 cells treated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti- -Catenin monoclonal (lanes 1 & 2), anti-Catenin alpha 1 (Tyr-148) phospho-specific (lanes 3 & 4), or anti-Catenin alpha 1 (a.a. 143-153) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23196_rabbit-catenin-alpha-1-p-tyr148-pab-1992023185692.jpg "Western Blot: Catenin alpha 1 [p Tyr148] Antibody [NBP3-23196] - Western blot analysis of rat PC12 cells treated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti- -Catenin monoclonal (lanes 1 & 2), anti-Catenin alpha 1 (Tyr-148) phospho-specific (lanes 3 & 4), or anti-Catenin alpha 1 (a.a. 143-153) (lanes 5 & 6).")
![Western Blot: Catenin alpha 1 Antibody [NBP3-23184] - Western blot analysis of rat PC12 cells treated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti- -Catenin monoclonal (lanes 1 & 2), anti-Catenin alpha 1 (Tyr-148) phospho-specific (lanes 3 & 4), or anti-Catenin alpha 1 (a.a. 143-153) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23184_rabbit-catenin-alpha-1-pab-199202312523617.jpg "Western Blot: Catenin alpha 1 Antibody [NBP3-23184] - Western blot analysis of rat PC12 cells treated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti- -Catenin monoclonal (lanes 1 & 2), anti-Catenin alpha 1 (Tyr-148) phospho-specific (lanes 3 & 4), or anti-Catenin alpha 1 (a.a. 143-153) (lanes 5 & 6).")
![Western Blot: EEF2K [p Ser445] Antibody [NBP3-23311] - Western blot image of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1-6), then some lanes of the blot were treated with lambda phosphatase (lanes 2, 4 & 6). The blot was probed with rabbit polyclonals anti-EEF2K (C-terminus) (lanes 1 & 2), anti-EEF2K (Ser-445) phospho-specific (lanes 3 & 4), and anti-EEF2K (Ser-398) phospho-specific (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23311_rabbit-eef2k-p-ser445-pab-19920231410147.jpg "Western Blot: EEF2K [p Ser445] Antibody [NBP3-23311] - Western blot image of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1-6), then some lanes of the blot were treated with lambda phosphatase (lanes 2, 4 & 6). The blot was probed with rabbit polyclonals anti-EEF2K (C-terminus) (lanes 1 & 2), anti-EEF2K (Ser-445) phospho-specific (lanes 3 & 4), and anti-EEF2K (Ser-398) phospho-specific (lanes 5 & 6).")
![Western Blot: RelB Antibody [NBP1-22980] - Whole cell lysate from Jurkat, HeLa and mouse NIH3T3 cells. RelB was also immunoprecipitated by rabbit anti-RelB antibody NBP1-22979.](http://images.novusbio.com/fullsize/RelB-Antibody-Western-Blot-NBP1-22980-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: RelB Antibody [NBP1-22980] - Whole cell lysate from Jurkat, HeLa and mouse NIH3T3 cells. RelB was also immunoprecipitated by rabbit anti-RelB antibody NBP1-22979.")