| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98814-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89184-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50171-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26681-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-48005-0.1mg | |||
![Western Blot: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Western blot analysis of HGFR/c-MET on different lysates with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/500 dilution.<br/><br/> Lane 1: A549 cell lysate<br/> Lane 2: HepG2 cell lysate<br/> Lane 3: HT-29 cell lysate<br/> Lane 4: Hela cell lysate<br/> Lane 5: LO2 cell lysate<br/> Lane 6: SK-Br-3 cell lysate (Negative)<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 156 kDa<br/> Observed band size: 145 kDa<br/><br/> Exposure time: 1 minute;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32198) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32198_mouse-hgfr-c-met-mab-a9a4-r-278202416331273.jpg "Western Blot: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Western blot analysis of HGFR/c-MET on different lysates with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/500 dilution.<br/><br/> Lane 1: A549 cell lysate<br/> Lane 2: HepG2 cell lysate<br/> Lane 3: HT-29 cell lysate<br/> Lane 4: Hela cell lysate<br/> Lane 5: LO2 cell lysate<br/> Lane 6: SK-Br-3 cell lysate (Negative)<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 156 kDa<br/> Observed band size: 145 kDa<br/><br/> Exposure time: 1 minute;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32198) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/400 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32198) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32198_mouse-hgfr-c-met-mab-a9a4-r-29820241416182.jpg)
![Western Blot: PIK3CA Antibody [NBP2-98814] - Lane A: Mouse heart tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:124 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Western-Blot-NBP2-98814-img0002.jpg "Western Blot: PIK3CA Antibody [NBP2-98814] - Lane A: Mouse heart tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:124 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody [NBP2-98814] - Immunofluorescence staining of PIK3CA in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98814 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98814_rabbit-polyclonal-pik3ca-antibody-235202511444496.png)
![Western Blot: ErbB2/Her2 Antibody (PD00-99) [NBP3-32423] - Western blot analysis of ErbB2/Her2 on SK-Br-3 cell lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32423) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 138 kDa<br/> Observed band size: 180 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32423) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32423_rabbit-erbb2-her2-mab-pd00-99-278202415242676.jpg "Western Blot: ErbB2/Her2 Antibody (PD00-99) [NBP3-32423] - Western blot analysis of ErbB2/Her2 on SK-Br-3 cell lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32423) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 138 kDa<br/> Observed band size: 180 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 6% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32423) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ErbB2/Her2 Antibody (PD00-99) [NBP3-32423] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-ErbB2/Her2 antibody (NBP3-32423) at 1/4,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32423) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32423_rabbit-erbb2-her2-mab-pd00-99-298202414333621.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunocytochemistry-Immunofluorescence-NBP2-89184-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of human ERBB2 in human breast carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunohistochemistry-Paraffin-NBP2-89184-img0002.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (24D2) [NBP3-09136] - Separation of MCF-7 cells stained using anti-human CD340 (24D2) purified antibody (concentration in sample 5,0 ug/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-24D2-Flow-Cytometry-NBP3-09136-img0001.jpg "Flow Cytometry: ErbB2/Her2 Antibody (24D2) [NBP3-09136] - Separation of MCF-7 cells stained using anti-human CD340 (24D2) purified antibody (concentration in sample 5,0 ug/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).")
![Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Immunocytochemistry analysis of A431 cells treated with or without 100ng/mL EGF for 10 minutes labeling ErbB2/Her2 with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-29202410484847.jpg "Immunocytochemistry/ Immunofluorescence: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Immunocytochemistry analysis of A431 cells treated with or without 100ng/mL EGF for 10 minutes labeling ErbB2/Her2 with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ErbB2/Her2 antibody (NBP3-32768) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: ErbB2/Her2 [p Tyr1139] Antibody (JE60-90) [NBP3-32768] - Flow cytometric analysis of A431 cells treated with or without 100ng/mL EGF for 10 minutes labeling ErbB2/Her2.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32768, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32768_rabbit-erbb2-her2-p-tyr1139-mab-je60-90-2920241637217.jpg)
![Immunoprecipitation: HGF R/c-MET Antibody (17) [NBP2-50171] - Immunoprecipitation of SNU-5 cell lysates using anti-c-Met [17]. Detection was performed by antibodies SC-10 and AF276.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-17-Immunoprecipitation-NBP2-50171-img0001.jpg "Immunoprecipitation: HGF R/c-MET Antibody (17) [NBP2-50171] - Immunoprecipitation of SNU-5 cell lysates using anti-c-Met [17]. Detection was performed by antibodies SC-10 and AF276.")
![Immunohistochemistry-Paraffin: Ret Antibody (C1) [NBP3-42078] - DAB staining on IHC-P; Sample: Mouse Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-42078; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42078_mouse-ret-mab-c1-612202421331684.jpg "Immunohistochemistry-Paraffin: Ret Antibody (C1) [NBP3-42078] - DAB staining on IHC-P; Sample: Mouse Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-42078; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Western Blot: Ret Antibody (C1) [NBP3-42078] - Sample: K562 cell lysate Primary Ab: 3ug/ml NBP3-42078; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42078_mouse-ret-mab-c1-61220242126298.jpg)
![Immunohistochemistry: CD44 Antibody (4B7) [NBP3-26681] - Image of CD44 Antibody (4B7) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26681_rabbit-cd44-mab-4b7-27220248203910.jpg "Immunohistochemistry: CD44 Antibody (4B7) [NBP3-26681] - Image of CD44 Antibody (4B7) diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: CD44 Antibody (4B7) [NBP3-26681] - Positive Western Blot detected in Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate.<br /> All lanes: CD44 Antibody at 1.25ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 82 KDa<br /> Observed band size: 82 KDa<br />](http://images.novusbio.com/fullsize/nbp3-26681_rabbit-cd44-mab-4b7-2822024114098.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/273) [NBP2-44984] - ICC/IF staining of MCF-7 cells with followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-273-Immunofluorescence-NBP2-44984-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/273) [NBP2-44984] - ICC/IF staining of MCF-7 cells with followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).")