| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89184-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90077-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98814-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50171-0.025ml | |||
![Western Blot: HIST1H3A [ac Lys9] Antibody (464) [NBP2-43733] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr).B. 30 ug HeLa whole cell lysate/extract (0.4 uM Thicostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H3K9ac (acetyl Lys9) antibody [464].](http://images.novusbio.com/fullsize/HIST1H3A-ac-Lys9-Antibody-464-Western-Blot-NBP2-43733-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIST1H3A [ac Lys9] Antibody (464) [NBP2-43733] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr).B. 30 ug HeLa whole cell lysate/extract (0.4 uM Thicostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H3K9ac (acetyl Lys9) antibody [464].")
![<b>Biological Strategies Validation. </b>Histone H3K9ac (acetyl Lys9) antibody [GT464] detects Histone H3K9ac (acetyl Lys9) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H3K9ac (acetyl Lys9) protein stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] (NBP2-43733) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-43733_mouse-monoclonal-hist1h3a-ac-lys9-antibody-464-immunocytochemistry-immunofluorescence-2611202518413911.jpg)
![Western Blot: PIM1 Antibody (S08-8D5) [NBP3-14963] - Western blot detection of PIM1 in Hela, A549, HL-60 cell lysates using PIM1 Rabbit mAb (1:1000 diluted). Predicted band size: 45kDa. Observed band size: 34kDa.](http://images.novusbio.com/fullsize/PIM1-Antibody-S08-8D5-Western-Blot-NBP3-14963-img0001.jpg "Western Blot: PIM1 Antibody (S08-8D5) [NBP3-14963] - Western blot detection of PIM1 in Hela, A549, HL-60 cell lysates using PIM1 Rabbit mAb (1:1000 diluted). Predicted band size: 45kDa. Observed band size: 34kDa.")
![Immunohistochemistry-Paraffin: PIM1 Antibody (S08-8D5) [NBP3-14963] - Immunohistochemistry of PIM1 in paraffin-embedded Human tonsil using PIM1 Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/PIM1-Antibody-S08-8D5-Immunohistochemistry-Paraffin-NBP3-14963-img0002.jpg)
![Western Blot: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1221/Tyr1222) antibody (NBP3-13232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1222--p-Tyr1221]-Antibody-Western-Blot-NBP3-13232-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1222, p Tyr1221] Antibody [NBP3-13232] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1221/Tyr1222) antibody (NBP3-13232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Western blot analysis of HGFR/c-MET on different lysates with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32198) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32198_mouse-hgfr-c-met-mab-a9a4-r-278202416331273.jpg "Western Blot: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Western blot analysis of HGFR/c-MET on different lysates with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/500 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HT-29 cell lysate Lane 4: Hela cell lysate Lane 5: LO2 cell lysate Lane 6: SK-Br-3 cell lysate (Negative) Lysates/proteins at 30 ug/Lane. Predicted band size: 156 kDa Observed band size: 145 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32198) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HGFR/c-MET Antibody (A9A4-R) [NBP3-32198] - Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue with Mouse anti-HGFR/c-MET antibody (NBP3-32198) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32198) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32198_mouse-hgfr-c-met-mab-a9a4-r-29820241416182.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunocytochemistry-Immunofluorescence-NBP2-89184-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of human ERBB2 in human breast carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunohistochemistry-Paraffin-NBP2-89184-img0002.jpg)
![Western Blot: CD44 Antibody (065) [NBP2-90077] - Lane A: HL60 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:82 kDaObserved band size:82 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/CD44-Antibody-065-Western-Blot-NBP2-90077-img0001.jpg "Western Blot: CD44 Antibody (065) [NBP2-90077] - Lane A: HL60 Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:82 kDaObserved band size:82 kDa(We are unsure as to the identity of these extra bands.)")
![Western Blot: ErbB2/Her2 Antibody (PD00-99) [NBP3-32423] - Western blot analysis of ErbB2/Her2 on SK-Br-3 cell lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32423) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32423) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32423_rabbit-erbb2-her2-mab-pd00-99-278202415242676.jpg "Western Blot: ErbB2/Her2 Antibody (PD00-99) [NBP3-32423] - Western blot analysis of ErbB2/Her2 on SK-Br-3 cell lysates with Rabbit anti-ErbB2/Her2 antibody (NBP3-32423) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 138 kDa Observed band size: 180 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32423) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ErbB2/Her2 Antibody (PD00-99) [NBP3-32423] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-ErbB2/Her2 antibody (NBP3-32423) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32423) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32423_rabbit-erbb2-her2-mab-pd00-99-298202414333621.jpg)
![Western Blot: PIK3CA Antibody [NBP2-98814] - Lane A: Mouse heart tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:124 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/PIK3CA-Antibody-Western-Blot-NBP2-98814-img0002.jpg "Western Blot: PIK3CA Antibody [NBP2-98814] - Lane A: Mouse heart tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:124 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: PIK3CA Antibody [NBP2-98814] - Immunofluorescence staining of PIK3CA in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98814 antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-98814_rabbit-polyclonal-pik3ca-antibody-235202511444496.png)
![Immunoprecipitation: HGF R/c-MET Antibody (17) [NBP2-50171] - Immunoprecipitation of SNU-5 cell lysates using anti-c-Met [17]. Detection was performed by antibodies SC-10 and AF276.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-17-Immunoprecipitation-NBP2-50171-img0001.jpg "Immunoprecipitation: HGF R/c-MET Antibody (17) [NBP2-50171] - Immunoprecipitation of SNU-5 cell lysates using anti-c-Met [17]. Detection was performed by antibodies SC-10 and AF276.")