| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89184-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05382-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52639-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26574-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50116-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunocytochemistry-Immunofluorescence-NBP2-89184-img0001.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4c overnight. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody ( green ) and counterstained with DAPI ( blue ). Positive staining was localized to plasma membrane.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (002) [NBP2-89184] - Staining of human ERBB2 in human breast carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-002-Immunohistochemistry-Paraffin-NBP2-89184-img0002.jpg)
![Western Blot: FGFR1 [p Tyr653] Antibody [NBP3-05382] - Western blot analysis of extracts of 293T cells, using FGFR1 pAb (NBP3-05382) at 1:1000 dilution.293T cells were treated by EGF (25 ug/mL) at 37? for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/FGFR1-[p-Tyr653]-Antibody-Western-Blot-NBP3-05382-img0001.jpg "Western Blot: FGFR1 [p Tyr653] Antibody [NBP3-05382] - Western blot analysis of extracts of 293T cells, using FGFR1 pAb (NBP3-05382) at 1:1000 dilution.293T cells were treated by EGF (25 ug/mL) at 37? for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (4D5-8 (trastuzumab)) - Chimeric [NBP2-52639] - Immunohistochemical Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) (NBP2-52639). Primary antibody incubation with NBP2-52639 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/ml.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-4D5-8-trastuzumab-Chimeric-Immunohistochemistry-Paraffin-NBP2-52639-img0001.jpg "Immunohistochemistry-Paraffin: ErbB2/Her2 Antibody (4D5-8 (trastuzumab)) - Chimeric [NBP2-52639] - Immunohistochemical Anti-erbB-2 (Her-2/neu) staining of paraffin embedded human breast tissue using the rabbit-chimeric version of 4D5-8 (trastuzumab) (NBP2-52639). Primary antibody incubation with NBP2-52639 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Weak membrane staining exclusively of ductal epithelial cells may be observed. Recommended concentration, 4-6 ug/ml.")
![Immunohistochemistry: HIST1H3A [Formyl Lys122] Antibody (32C1) [NBP3-26574] - Image of HIST1H3A [Formyl Lys122] Antibody (32C1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.09% DAB.](http://images.novusbio.com/fullsize/nbp3-26574_rabbit-hist1h3a-formyl-lys-122-mab-32c1-262202414471535.jpg "Immunohistochemistry: HIST1H3A [Formyl Lys122] Antibody (32C1) [NBP3-26574] - Image of HIST1H3A [Formyl Lys122] Antibody (32C1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.09% DAB.")
![Immunohistochemistry: HIST1H3A [Formyl Lys122] Antibody (32C1) [NBP3-26574] - Image of HIST1H3A [Formyl Lys122] Antibody (32C1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.09% DAB.](http://images.novusbio.com/fullsize/nbp3-26574_rabbit-hist1h3a-formyl-lys-122-mab-32c1-26220241525332.jpg)
![Western Blot: TrkB [p Tyr705] Antibody (S04-6B1) [NBP3-14902] - Western blot detection of TrkB [p Tyr705] in Hela, A549, C6 cell lysates using TrkB [p Tyr705] Rabbit mAb (1:1000 diluted). Predicted band size: 92kDa. Observed band size: 140, 90kDa.](http://images.novusbio.com/fullsize/TrkB-p-Tyr705-Antibody-S04-6B1-Western-Blot-NBP3-14902-img0001.jpg "Western Blot: TrkB [p Tyr705] Antibody (S04-6B1) [NBP3-14902] - Western blot detection of TrkB [p Tyr705] in Hela, A549, C6 cell lysates using TrkB [p Tyr705] Rabbit mAb (1:1000 diluted). Predicted band size: 92kDa. Observed band size: 140, 90kDa.")
![Western Blot: TrkB Antibody (S01-8H1) [NBP3-19481] - Western blot detection of TrkB (pan) in Rat Brain cell using TrkB (pan) Rabbit mAb (1:1000 diluted). Predicted band size: 92kDa. Observed band size: 120-140kDa.](http://images.novusbio.com/fullsize/TrkB-Antibody-S01-8H1-Western-Blot-NBP3-19481-img0001.jpg "Western Blot: TrkB Antibody (S01-8H1) [NBP3-19481] - Western blot detection of TrkB (pan) in Rat Brain cell using TrkB (pan) Rabbit mAb (1:1000 diluted). Predicted band size: 92kDa. Observed band size: 120-140kDa.")
![Western Blot: EEF1A2 [p Ser358] Antibody [NBP2-29510] - Western blot of mouse hippocampus lysate showing specific immunolabeling of the ~ 50k eEF1A2 protein phosphorylated at Ser358. Immunolabeling is blocked by treatment of the lysate with lambda phosphatase (P-tase).](http://images.novusbio.com/fullsize/EEF1A2-[p-Ser358]-Antibody-Western-Blot-NBP2-29510-img0001.jpg "Western Blot: EEF1A2 [p Ser358] Antibody [NBP2-29510] - Western blot of mouse hippocampus lysate showing specific immunolabeling of the ~ 50k eEF1A2 protein phosphorylated at Ser358. Immunolabeling is blocked by treatment of the lysate with lambda phosphatase (P-tase).")
![Western Blot: FAM129B [p Ser683] Antibody [NBP2-60779] - Western blot of 3T3 cell lysate showing specific immunolabeling of the ~83 kDa FAM129B protein phosphorylated at Ser679/683 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).](http://images.novusbio.com/fullsize/FAM129B-[p-Ser683]-Antibody-Western-Blot-NBP2-60779-img0001.jpg "Western Blot: FAM129B [p Ser683] Antibody [NBP2-60779] - Western blot of 3T3 cell lysate showing specific immunolabeling of the ~83 kDa FAM129B protein phosphorylated at Ser679/683 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).")
![Western Blot: PDGF R beta [p Tyr1021] Antibody (JE48-54) [NBP3-32786] - Western blot analysis of PDGF R beta on different lysates with Rabbit anti-PDGF R beta antibody (NBP3-32786) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 100 ng/mL PDGF for 5 minutes whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 124 kDa Observed band size: 190 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32786) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32786_rabbit-pdgf-r-beta-p-tyr1021-mab-je48-54-288202412442998.jpg "Western Blot: PDGF R beta [p Tyr1021] Antibody (JE48-54) [NBP3-32786] - Western blot analysis of PDGF R beta on different lysates with Rabbit anti-PDGF R beta antibody (NBP3-32786) at 1/1,000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 100 ng/mL PDGF for 5 minutes whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 124 kDa Observed band size: 190 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32786) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")