| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05533-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21673-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17658-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21414-25ul | |||
![Western Blot: Fibrillarin Antibody [NBP3-05533] - Western blot analysis of different tissue and cell line lysates using Fibrillarin Antibody at a dilution of 1:2,000, in green: [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35kDa corresponds to Fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since Fibrillarin is more heavily expressed in rapidly dividing cells. The blot simultaneously was probed with rabbit pAb to UCHL1 at a dilution of 1:3,000, in red, revealing the ~25kDa UCHL1 protein in lysates of tissues containing neurons or cells with neuronal properties.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP3-05533-img0002.jpg "Western Blot: Fibrillarin Antibody [NBP3-05533] - Western blot analysis of different tissue and cell line lysates using Fibrillarin Antibody at a dilution of 1:2,000, in green: [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35kDa corresponds to Fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since Fibrillarin is more heavily expressed in rapidly dividing cells. The blot simultaneously was probed with rabbit pAb to UCHL1 at a dilution of 1:3,000, in red, revealing the ~25kDa UCHL1 protein in lysates of tissues containing neurons or cells with neuronal properties.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP3-05533] - Immunofluorescent analysis of (A) C6 rat glioma cells and (B) HEK293 human embryonic kidney cells stained with Fibrillarin Antibody at a dilution of 1:1,000 in red, in both cases costained with chicken pAb to vimentin at a dilution of 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The Fibrillarin antibody detects Fibrillarin protein localized in nucleoli while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05533-img0001.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - Fibrillarin antibody immunoprecipitates FBL protein in IP experiments. IP samples: HeLa nuclear extract. A. 35 ug HeLa nuclear extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of FBL protein by 4 ug Fibrillarin antibody. 10 % SDS-PAGE. The immunoprecipitated FBL protein was detected by Fibrillarin antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0005.jpg "Western Blot: Fibrillarin Antibody [NBP2-16492] - Fibrillarin antibody immunoprecipitates FBL protein in IP experiments. IP samples: HeLa nuclear extract. A. 35 ug HeLa nuclear extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of FBL protein by 4 ug Fibrillarin antibody. 10 % SDS-PAGE. The immunoprecipitated FBL protein was detected by Fibrillarin antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP2-16492] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibrillarin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16492-img0004.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - 30 ug Rat whole cell lysate/extract 12 % SDS-PAGE Fibrillarin antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0001.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0002.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-16492] - Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-16492-img0003.jpg)
![Western Blot: Fibrillarin Antibody (SR1545) [NBP3-21673] - Western blot analysis using NBP3-21673 in HepG2 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21673_rabbit-fibrillarin-mab-sr1545-57202317215153.jpg "Western Blot: Fibrillarin Antibody (SR1545) [NBP3-21673] - Western blot analysis using NBP3-21673 in HepG2 cell lysate.")
![Immunohistochemistry: Fibrillarin Antibody (SR1545) [NBP3-21673] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21673.](http://images.novusbio.com/fullsize/nbp3-21673_rabbit-fibrillarin-mab-sr1545-5720231754866.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (SR1545) [NBP3-21673] - Immunofluorescent analysis of Hela cells, using NBP3-21673.](http://images.novusbio.com/fullsize/nbp3-21673_rabbit-fibrillarin-mab-sr1545-113202515145423.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - Detection of Human THOC1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THOC1 antibody NBP1-47304 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP1-47304-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - Detection of Human THOC1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THOC1 antibody NBP1-47304 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- THOC1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-NBP1-47304-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP1-47304] - Detection of human THOC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-THOC1 antibody NBP1-47304 used for IP at 3 ug per reaction. THOC1 was also immunoprecipitated by another rabbit anti-THOC1 antibody. For blotting immunoprecipitated THOC1, NBP1-47304 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunoprecipitation-NBP1-47304-img0006.jpg)
![Western Blot: H1F0 Antibody (S07-0E8) [NBP3-19961] - Western blot detection of H1F0 in C6 (Lane 1), CHO-K1 (Lane 2) cell lysates using NBP3-19961 (1:1000 diluted). Predicted band size: 21kDa. Observed band size: 30kDa.](http://images.novusbio.com/fullsize/H1F0-Antibody-S07-0E8-Western-Blot-NBP3-19961-img0002.jpg "Western Blot: H1F0 Antibody (S07-0E8) [NBP3-19961] - Western blot detection of H1F0 in C6 (Lane 1), CHO-K1 (Lane 2) cell lysates using NBP3-19961 (1:1000 diluted). Predicted band size: 21kDa. Observed band size: 30kDa.")
![Immunohistochemistry-Paraffin: H1F0 Antibody (S07-0E8) [NBP3-19961] - Immunohistochemistry of H1F0 in paraffin-embedded Human Cholangiocarcinoma using NBP3-19961 at dilution 1/50](http://images.novusbio.com/fullsize/H1F0-Antibody-S07-0E8-Immunohistochemistry-Paraffin-NBP3-19961-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (S05-1A8) [NBP3-14940] - Immunofluorescence of hHpr1-p84-Thoc1 (green) in Hela cells using hHpr1-p84-Thoc1 Rabbit mAb at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-S05-1A8-Immunocytochemistry-Immunofluorescence-NBP3-14940-img0001.jpg "Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (S05-1A8) [NBP3-14940] - Immunofluorescence of hHpr1-p84-Thoc1 (green) in Hela cells using hHpr1-p84-Thoc1 Rabbit mAb at dilution 1/50, and DAPI (blue)")
![Western Blot: hHpr1-p84-Thoc1 Antibody (S05-1A8) [NBP3-14940] - Western blot in Jurkat, Hela, COS7 cell lysates using NBP3-14940 (1:1000 diluted). Predicted band size: 76KDa. Observed band size: 84KDa.](http://images.novusbio.com/fullsize/nbp3-14940_rabbit-hhpr1-p84-thoc1-mab-s05-1a8-234202415232522.jpg)
![Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (S05-1A8) [NBP3-14940] - Immunofluorescence of hHpr1-p84-Thoc1 (green) in Hela cells using NBP3-14940 at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-14940_rabbit-hhpr1-p84-thoc1-mab-s05-1a8-113202515145439.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-26151] - NBP2-26151 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-26151-img0001.jpg "Western Blot: Fibrillarin Antibody [NBP2-26151] - NBP2-26151 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Histone H1 Antibody [NBP3-17658] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Histone-H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17658-img0003.jpg "Immunocytochemistry/Immunofluorescence: Histone H1 Antibody [NBP3-17658] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: Histone H1 Antibody [NBP3-17658] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Histone-H1-Antibody-Immunohistochemistry-Paraffin-NBP3-17658-img0001.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21414_rabbit-fibrillarin-pab-45202315524516.jpg "Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil")
![Western Blot: Fibrillarin Antibody [NBP1-57272] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP1-57272-img0004.jpg "Western Blot: Fibrillarin Antibody [NBP1-57272] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml")
![Immunohistochemistry-Paraffin: Fibrillarin Antibody [NBP1-57272] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunohistochemistry-Paraffin-NBP1-57272-img0003.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-26150] - NBP2-26150 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-26150-img0001.jpg "Western Blot: Fibrillarin Antibody [NBP2-26150] - NBP2-26150 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunoprecipitation:H1F0 AntibodyNBP3-30401] -Detection of human H1F0/Histone H1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-H1F0/Histone H1.0 antibody NBP3-30401 (lot NBP3-30401-1) used for IP at 6 µg per reaction. H1F0/Histone H1.0 was also immunoprecipitated by rabbit anti-H1F0/Histone H1.0 antibody. For blotting immunoprecipitated H1F0/Histone H1.0, NBP3-30401 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30401_rabbit-h1f0-pab-30122024192471.jpg "Immunoprecipitation:H1F0 AntibodyNBP3-30401] -Detection of human H1F0/Histone H1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-H1F0/Histone H1.0 antibody NBP3-30401 (lot NBP3-30401-1) used for IP at 6 µg per reaction. H1F0/Histone H1.0 was also immunoprecipitated by rabbit anti-H1F0/Histone H1.0 antibody. For blotting immunoprecipitated H1F0/Histone H1.0, NBP3-30401 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")