| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34565-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47932-0.1mg | |||
| IHC-Prediluted | NBP2-48315 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54422-100ug | |||
![Western Blot: GABA-A R alpha 2 Antibody [NBP2-36560] - Western blot of mouse brain lysates from wild type (Control) and alpha2-knockout (alpha2-K/O) animals showing specific immunolabeling of the ~51k alpha2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha2-knockout animals.](http://images.novusbio.com/fullsize/GABA-A-R-alpha-2-Antibody-Western-Blot-NBP2-36560-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GABA-A R alpha 2 Antibody [NBP2-36560] - Western blot of mouse brain lysates from wild type (Control) and alpha2-knockout (alpha2-K/O) animals showing specific immunolabeling of the ~51k alpha2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha2-knockout animals.")
![Immunoprecipitation: xCT Antibody (JE31-93) [NBP3-33014] - xCT was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33014 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33014 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: NBP3-33014 IP in HeLa cell lysate<br/> Lane 3: Rabbit IgG instead of NBP3-33014 in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 24 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-33014_rabbit-xct-mab-je31-93-26820241582617.jpg "Immunoprecipitation: xCT Antibody (JE31-93) [NBP3-33014] - xCT was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33014 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33014 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: NBP3-33014 IP in HeLa cell lysate<br/> Lane 3: Rabbit IgG instead of NBP3-33014 in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 24 seconds; ECL")
![Western blot analysis on different lysates with NBP3-33014 at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: 293T cell lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (40 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Rat brain tissue lysate (40 µg/Lane) Lane 7: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33014 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33014_-western-blot-639204597142561564.jpg)
![Western blot analysis of PC-12 cell lysates with NBP3-33014 at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-33014 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33014_-western-blot-639204598085499933.jpg)
![Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32698_mouse-p53-mab-6b5-r-29202410484875.jpg "Immunocytochemistry/ Immunofluorescence: p53 Antibody (6B5-R) [NBP3-32698] - Immunocytochemistry analysis of A431 cells labeling p53 with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-p53 antibody (NBP3-32698) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Western blot analysis on different lysates with NBP3-32698 at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HT-29 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 0.5μM doxorubicincell for 24 hours lysate Lane 5: Neuro-2a cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: Lane 1-2: 1 minute 40 seconds; Lane 3-6: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32698 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_-western-blot-639204594545626873.jpg)
![Western blot analysis on different lysates with NBP3-32698 at 1/2,000 dilution. Lane 1: A431 cell lysate Lane 2: MDA-MB-231 cell lysate Lane 3: Saos-2 cell lysate (negative) Lysates/proteins at 30 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32698 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat anti-Mouse IgG [HRP] at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32698_-western-blot-639204597793269876.jpg)
![Western Blot: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Western-Blot-NBP2-67207-img0001.jpg "Western Blot: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMKII alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Immunohistochemistry-Paraffin-NBP2-67207-img0004.jpg)
![Flow Cytometry: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Flow cytometric analysis of CaM Kinase II alpha was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Flow-Cytometry-NBP2-67207-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-CaM Kinase II alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Immunohistochemistry-Paraffin-NBP2-67207-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (M401) [NBP3-23224] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23224_mouse-nnos-mab-m401-19920231856948.jpg "Immunocytochemistry/Immunofluorescence: nNOS Antibody (M401) [NBP3-23224] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: nNOS Antibody (M401) [NBP3-23224] - Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (NBP3-23224) (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (NBP3-23225) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23224_mouse-nnos-mab-m401-19920231820296.jpg)
![Immunohistochemistry-Paraffin: TRPM2 Antibody [NBP1-33416] - Paraffin-embedded rat liver. TRPM2 antibody [N1N2-2], N-term diluted at 1:500.](http://images.novusbio.com/fullsize/TRPM2-Antibody-Immunohistochemistry-Paraffin-NBP1-33416-img0004.jpg "Immunohistochemistry-Paraffin: TRPM2 Antibody [NBP1-33416] - Paraffin-embedded rat liver. TRPM2 antibody [N1N2-2], N-term diluted at 1:500.")
![Immunohistochemistry-Paraffin: TRPM2 Antibody [NBP1-33416] - Paraffin-embedded MDAMB468 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/TRPM2-Antibody-Immunohistochemistry-Paraffin-NBP1-33416-img0003.jpg)
![Western Blot: TRPM2 Antibody [NBP1-33416] - HepG2 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TRPM2 antibody [N1N2-2], N-term (NBP1-33416) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-33416_rabbit-polyclonal-trpm2-antibody-91020242041291.jpg)
![HepG2 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with TRPM2 antibody [N1N2-2], N-term (NBP1-33416) diluted at 1:500 and competitor's antibody (ACC-043) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.<br><br>*The competitor is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp1-33416_rabbit-polyclonal-trpm2-antibody-western-blot-2611202518491318.jpg)
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD469) [NBP2-32918] - Formalin-fixed, paraffin-embedded mouse intestine tissue stained with BrdU MAb (BRD469).](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-BRD469-Immunohistochemistry-Paraffin-NBP2-32918-img0001.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD469) [NBP2-32918] - Formalin-fixed, paraffin-embedded mouse intestine tissue stained with BrdU MAb (BRD469).")
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD469) [NBP2-32918] - Formalin-fixed, paraffin-embedded Rat Kidney stained with AF488 conjugate of BrdU Monoclonal Antibody (BRD469).](http://images.novusbio.com/fullsize/Bromodeoxyuridine-BrdU-Antibody-BRD469-Immunohistochemistry-Paraffin-NBP2-32918-img0002.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (UCHL1/775) [NBP2-44795] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (UCHL1/775)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-UCHL1-775-Western-Blot-NBP2-44795-img0004.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (UCHL1/775) [NBP2-44795] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (UCHL1/775)")
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (UCHL1/775) [NBP2-44795] - Rat Cerebellum stained with Pgp9.5 Monoclonal Antibody (UCHL1/775).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-UCHL1-775-Immunohistochemistry-Paraffin-NBP2-44795-img0001.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (UCHL1/775) [NBP2-44795] - Rat Testis stained with Pgp9.5 Monoclonal Antibody (UCHL1/775).](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-UCHL1-775-Immunohistochemistry-Paraffin-NBP2-44795-img0002.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Western Blot Analysis of human Liver tissue lysate using Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381).](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-ALDH1A1-1381-Western-Blot-NBP2-53248-img0005.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Western Blot Analysis of human Liver tissue lysate using Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381).")
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Formalin-fixed, paraffin-embedded Human Testicular Carcinoma stained with ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-ALDH1A1-1381-Immunohistochemistry-Paraffin-NBP2-53248-img0002.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Western Blot of K562 and Lung lysate Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381).](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-ALDH1A1-1381-Western-Blot-NBP2-53248-img0004.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (ALDH1A1/1381) [NBP2-53248] - Formalin-fixed, paraffin-embedded Human Colon Carcinoma stained with ALDH1A1 Monoclonal Antibody (ALDH1A1/1381).](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-ALDH1A1-1381-Immunohistochemistry-Paraffin-NBP2-53248-img0001.jpg)