| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24691SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92241-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73030 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30423-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26441-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34141-25ul | |||
![Immunocytochemistry/Immunofluorescence: MNX1/HLXB9 Antibody [NBP2-24691] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MNX1/HLXB9 NBP2-24691 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24691-img0003.jpg "Immunocytochemistry/Immunofluorescence: MNX1/HLXB9 Antibody [NBP2-24691] - MCF7 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MNX1/HLXB9 NBP2-24691 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunocytochemistry/Immunofluorescence: MNX1/HLXB9 Antibody [NBP2-24691] - Neuro2a cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MNX1/HLXB9 NBP2-24691 at 1 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24691-img0004.jpg)
![Western Blot: MNX1/HLXB9 Antibody [NBP2-24691] - Analysis of MNX1/HLXB9 in MOLT-4 cell lysates in the 1) absence and 2) presence of immunizing peptide using this antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Western-Blot-NBP2-24691-img0002.jpg)
![Immunohistochemistry-Paraffin: MNX1/HLXB9 Antibody [NBP2-24691] - Analysis of MNX1/HLXB9 in FFPE mouse pancreas tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Immunohistochemistry-Paraffin-NBP2-24691-img0001.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249432833.jpg "Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249415999.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-212024943553.jpg)
![Western Blot: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - Various extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249374992.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249374949.jpg)
![Western Blot: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249432886.jpg)
![Immunocytochemistry/Immunofluorescence: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in ice-cold MeOH for 5 min. Green: MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249342758.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody (HL1471) - Azide and BSA Free [NBP3-25558] - MC1 Receptor antibody [HL1471] detects MC1 Receptor protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded A375 xenograft . MC1 Receptor stained by MC1 Receptor antibody [HL1471] (NBP3-25558) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-2120249362712.jpg)
![Frozen-sectioned mouse cerebellum.Green: MC1 Receptor stained by MC1 Receptor antibody [HL1471] diluted at 1:100.Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-immunohistochemistry-frozen-119202513498.png)
![<b>Independent Antibodies Validation. </b>Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:50000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-141120251843244.jpg)
![<b>Independent Antibodies Validation. </b>Various tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-1411202518433316.jpg)
![<b>Independent Antibodies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Non-transfected (-) and transfected (+) unboiled A375 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1471] (NBP3-25558) diluted at 1:5000 and competitor's antibody (Highly Cited Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25558_rabbit-melanocortin-1-r-mc1r-mab-hl1471-azide-bsa-free-western-blot-14112025183376.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-92241] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PANX1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-92241-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Pannexin-1 Antibody [NBP1-92241] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PANX1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Pannexin-1 Antibody [NBP1-92241] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92241-img0010.jpg)
![Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human small intestine shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-92241-img0016.jpg)
![Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-92241-img0012.jpg)
![Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-92241-img0014.jpg)
![Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-92241] - Staining of human pancreas shows negative to very weak positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-92241-img0015.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-59672] - Human Jurkat cell lysate Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0010.jpg "Western Blot: Pannexin-1 Antibody [NBP1-59672] - Human Jurkat cell lysate Antibody Dilution: 1.0 ug/ml.")
![Immunohistochemistry: Pannexin-1 Antibody [NBP1-59672] - Paraffin Embedded Tissue: Human neural cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-NBP1-59672-img0008.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-59672] - Tissue: Hela whole cell lysates](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0004.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-59672] - PANX1 Antibody Titration: 0.2-1 ug/ml Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0001.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-59672] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-59672] - Mouse Retina and Knockout PANX-1 Mouse Tissue.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-59672-img0003.jpg)
![Immunohistochemistry: Pannexin-1 Antibody [NBP1-59672] - Human Intestine](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-NBP1-59672-img0007.jpg)
![Western Blot: Noggin Antibody (OTI1H8) [NBP1-47881] - Noggin Antibody (1H8) [NBP1-47881] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Noggin monoclonal antibody.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Western-Blot-NBP1-47881-img0012.jpg "Western Blot: Noggin Antibody (OTI1H8) [NBP1-47881] - Noggin Antibody (1H8) [NBP1-47881] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Noggin monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Noggin Antibody (OTI1H8) [NBP1-47881] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Noggin.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Immunocytochemistry-NBP1-47881-img0007.jpg)
![Immunohistochemistry-Paraffin: Noggin Antibody (1H8) [NBP1-47881] - Staining of paraffin-embedded Human Ovary tissue using anti-Noggin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Immunohistochemistry-Paraffin-NBP1-47881-img0009.jpg)
![Western Blot: Noggin Antibody (1H8) [NBP1-47881] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Noggin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Noggin.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Western-Blot-NBP1-47881-img0010.jpg)
![Western Blot: Noggin Antibody (1H8) [NBP1-47881] - Noggingin antibody (1H8) at 1:40000 + Recombinant human Noggingin.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Western-Blot-NBP1-47881-img0011.jpg)
![Immunohistochemistry-Paraffin: Noggin Antibody (1H8) [NBP1-47881] - Staining of paraffin-embedded Human lung tissue using anti-Noggin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Noggin-Antibody-1H8-Immunohistochemistry-Paraffin-NBP1-47881-img0008.jpg)
![Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1470] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-2592025864.png "Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1470] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:50000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251831322.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251828111.jpg)
![<b>Independent Antibodies Validation. </b>Various tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251831212.jpg)
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Non-transfected (-) and transfected (+) unboiled A375 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:5000 and competitor's antibody (Highly Cited Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251836367.jpg)
![Immunocytochemistry/Immunofluorescence: Lgi4 Antibody [NBP2-30423] - Staining of human cell line SK-MEL-30 shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/Lgi4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-30423-img0002.jpg "Immunocytochemistry/Immunofluorescence: Lgi4 Antibody [NBP2-30423] - Staining of human cell line SK-MEL-30 shows localization to the Golgi apparatus.")
![Immunohistochemistry-Paraffin: Lgi4 Antibody [NBP2-30423] -Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/antibody/nbp2-30423_rabbit-polyclonal-lgi4-antibody-immunohistochemistry-paraffin-127202494139..jpg)
![Immunohistochemistry-Paraffin: Lgi4 Antibody [NBP2-30423] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-30423_rabbit-polyclonal-lgi4-antibody-immunohistochemistry-paraffin-127202494239..jpg)
![Immunohistochemistry-Paraffin: Lgi4 Antibody [NBP2-30423] -Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/antibody/nbp2-30423_rabbit-polyclonal-lgi4-antibody-immunohistochemistry-paraffin-127202494335..jpg)
![Immunohistochemistry-Paraffin: Lgi4 Antibody [NBP2-30423] -Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-30423_rabbit-polyclonal-lgi4-antibody-immunohistochemistry-paraffin-127202494433..jpg)
![Western Blot: MNX1/HLXB9 Antibody [NBP3-13412] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MNX1/HLXB9 antibody (NBP3-13412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Western-Blot-NBP3-13412-img0003.jpg "Western Blot: MNX1/HLXB9 Antibody [NBP3-13412] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MNX1/HLXB9 antibody (NBP3-13412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Frozen: MNX1/HLXB9 Antibody [NBP3-13412] - MNX1/HLXB9 antibody detects MNX1/HLXB9 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Green: MNX1/HLXB9 stained by MNX1/HLXB9 antibody (NBP3-13412) diluted at 1:300. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Immunohistochemistry-Frozen-NBP3-13412-img0004.jpg)
![Western Blot: MNX1/HLXB9 Antibody [NBP3-13412] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MNX1/HLXB9 antibody (NBP3-13412) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Western-Blot-NBP3-13412-img0001.jpg)
![Western Blot: MNX1/HLXB9 Antibody [NBP3-13412] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MNX1/HLXB9 antibody (NBP3-13412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Western-Blot-NBP3-13412-img0002.jpg)
![Western Blot: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Western blot analysis of Pannexin-1 on different lysates with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C2C12 cell lysate (20 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32714) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-278202415212479.jpg "Western Blot: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Western blot analysis of Pannexin-1 on different lysates with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: U-87 MG cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: C2C12 cell lysate (20 ug/Lane) Lane 8: Rat brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Predicted band size: 48 kDa Observed band size: 50 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32714) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32714) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-298202413585529.jpg)
![Immunocytochemistry/ Immunofluorescence: Pannexin-1 Antibody (JE38-97) [NBP3-32714] - Immunocytochemistry analysis of 293T cells labeling Pannexin-1 with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pannexin-1 antibody (NBP3-32714) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32714_rabbit-pannexin-1-mab-je38-97-292024111486.jpg)
![Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-262202414225215.jpg "Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.")
![Immunohistochemistry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Image of Pannexin-1 Antibody (28G5) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.1:50% DAB.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-2722024847192.jpg)
![Immunocytochemistry/Immunofluorescence: Pannexin-1 Antibody (28G5) [NBP3-26441] - Staining of U251 with Pannexin-1 Antibody (28G5) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 502-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-282202412493632.jpg)
![Flow Cytometry: Pannexin-1 Antibody (28G5) [NBP3-26441] - Overlay Peak curve showing A549 cells stained with Pannexin-1 Antibody (28G5) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26441_rabbit-pannexin-1-mab-28g5-282202414312766.jpg)
![Western Blot: Noggin Antibody (A9) [NBP3-41765] - Sample: Mouse Placenta lysate Primary Ab: 1ug/ml NBP3-41765; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41765_mouse-noggin-mab-a9-61220242151879.jpg "Western Blot: Noggin Antibody (A9) [NBP3-41765] - Sample: Mouse Placenta lysate Primary Ab: 1ug/ml NBP3-41765; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: Noggin Antibody (A9) [NBP3-41765] - DAB staining on IHC-P; Samples: Human Glioma Tissue; Primary Ab: 30ug/ml NBP3-41765; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41765_mouse-noggin-mab-a9-612202421121379.jpg)
![Western Blot: Noggin Antibody (A9) [NBP3-41765] - Sample: Rat Placenta lysate; Primary Ab: 2ug/ml NBP3-41765; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41765_mouse-noggin-mab-a9-612202421395335.jpg)
![Immunohistochemistry-Paraffin: Noggin Antibody [NBP2-34141] - Staining of human prostate shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Noggin-Antibody-Immunohistochemistry-Paraffin-NBP2-34141-img0008.jpg "Immunohistochemistry-Paraffin: Noggin Antibody [NBP2-34141] - Staining of human prostate shows no positivity in glandular cells as expected.")
![Immunohistochemistry-Paraffin: Noggin Antibody [NBP2-34141] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/Noggin-Antibody-Immunohistochemistry-Paraffin-NBP2-34141-img0005.jpg)
![Immunohistochemistry-Paraffin: Noggin Antibody [NBP2-34141] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Noggin-Antibody-Immunohistochemistry-Paraffin-NBP2-34141-img0006.jpg)
![Immunohistochemistry-Paraffin: Noggin Antibody [NBP2-34141] - Staining of human placenta shows strong cytoplasmic positivity in decidual cells.](http://images.novusbio.com/fullsize/Noggin-Antibody-Immunohistochemistry-Paraffin-NBP2-34141-img0007.jpg)