| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77269-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77270-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82619-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-99606-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57365-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38089-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89828-50ul | |||
![Western Blot: Nicastrin Antibody [NBP1-77269] - Mouse brain tissue lysate with Nicastrin antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Western-Blot-NBP1-77269-img0002.jpg "Western Blot: Nicastrin Antibody [NBP1-77269] - Mouse brain tissue lysate with Nicastrin antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Nicastrin Antibody [NBP1-77269] - Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target protein (100x) Nicastrin. Overlay images indicate the possible co-localization of VDR/Nicastrin on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0188605) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77269-img0004.jpg)
![Immunohistochemistry-Paraffin: Nicastrin Antibody [NBP1-77269] - Human brain tissue with Nicastrin antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Immunohistochemistry-Paraffin-NBP1-77269-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77269] - Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77269_rabbit-polyclonal-nicastrin-antibody-2711202420175178.jpg)
![Western Blot: Nicastrin Antibody [NBP1-77270] - Human brain tissue lysate with Nicastrin antibody at (A) 0.5 and (B) 1ug/ml.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Western-Blot-NBP1-77270-img0002.jpg "Western Blot: Nicastrin Antibody [NBP1-77270] - Human brain tissue lysate with Nicastrin antibody at (A) 0.5 and (B) 1ug/ml.")
![Immunohistochemistry: Nicastrin Antibody - BSA Free [NBP1-77270] - Immunohistochemistry of Nicastrin in human brain tissue with Nicastrin antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77270_rabbit-polyclonal-nicastrin-antibody-271120241928886.jpg)
![Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77270] - Immunofluorescence of Nicastrin in mouse brain tissue with Nicastrin antibody at 20 ug/ml.Green: Nicastrin Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77270_rabbit-polyclonal-nicastrin-antibody-2711202420141926.gif)
![Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77270] - Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77270_rabbit-polyclonal-nicastrin-antibody-2711202419333434.jpg)
![Western Blot: PQBP1 Antibody [NBP1-82619] - Analysis in control (vector only transfected HEK293T lysate) and PQBP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/PQBP1-Antibody-Western-Blot-NBP1-82619-img0004.jpg "Western Blot: PQBP1 Antibody [NBP1-82619] - Analysis in control (vector only transfected HEK293T lysate) and PQBP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: PQBP1 Antibody [NBP1-82619] - Staining of human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PQBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82619-img0003.jpg)
![Immunohistochemistry-Paraffin: PQBP1 Antibody [NBP1-82619] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells and islet cells.](http://images.novusbio.com/fullsize/PQBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-82619-img0002.jpg)
![Western Blot: PQBP1 Antibody [NBP1-47271] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47271 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47270, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/PQBP1-Antibody-Western-Blot-NBP1-47271-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PQBP1 Antibody [NBP1-47271] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47271 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47270, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: PQBP1 Antibody [NBP1-47271] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 used at a dilution of 1:200 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit](http://images.novusbio.com/fullsize/PQBP1-Antibody-Immunohistochemistry-NBP1-47271-img0003.jpg)
![Western Blot: PQBP1 Antibody [NBP1-47270] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47270 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47271, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/PQBP1-Antibody-Western-Blot-NBP1-47270-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PQBP1 Antibody [NBP1-47270] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PQBP1 antibody NBP1-47270 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PQBP1 was also immunoprecipitated by rabbit anti-PQBP1 antibody NBP1-47271, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: PQBP1 Antibody [NBP1-47270] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-PQBP1 used at a dilution of 1:1,000 (1 ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit](http://images.novusbio.com/fullsize/PQBP1-Antibody-Immunohistochemistry-NBP1-47270-img0003.jpg)
![Western Blot: PQBP1 Antibody [NBP2-99606] - Anti-PQBP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 38 kDa](http://images.novusbio.com/fullsize/PQBP1-Antibody-Western-Blot-NBP2-99606-img0003.jpg "Western Blot: PQBP1 Antibody [NBP2-99606] - Anti-PQBP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 38 kDa")
![Immunocytochemistry/Immunofluorescence: PQBP1 Antibody [NBP2-99606] - Immunofluorescence staining of PQBP1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99606 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus .](http://images.novusbio.com/fullsize/nbp2-99606_rabbit-polyclonal-pqbp1-antibody-2352025154436.png)
![Immunocytochemistry/Immunofluorescence: PQBP1 Antibody (3H7) [H00010084-M15] - Analysis of monoclonal antibody to PQBP1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PQBP1-Antibody-3H7-Immunocytochemistry-Immunofluorescence-H00010084-M15-img0002.jpg "Immunocytochemistry/Immunofluorescence: PQBP1 Antibody (3H7) [H00010084-M15] - Analysis of monoclonal antibody to PQBP1 on HeLa cell. Antibody concentration 10 ug/ml")
![Sandwich ELISA: PQBP1 Antibody (3H7) [H00010084-M15] - Detection limit for recombinant GST tagged PQBP1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PQBP1-Antibody-3H7-Sandwich-ELISA-H00010084-M15-img0003.jpg)
![Western Blot: PQBP1 Antibody (1A11) [H00010084-M01] - Analysis of PQBP1 expression in transfected 293T cell line by PQBP1 monoclonal antibody (M01), clone 1A11.Lane 1: PQBP1 transfected lysate(30.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PQBP1-Antibody-1A11-Western-Blot-H00010084-M01-img0002.jpg "Western Blot: PQBP1 Antibody (1A11) [H00010084-M01] - Analysis of PQBP1 expression in transfected 293T cell line by PQBP1 monoclonal antibody (M01), clone 1A11.Lane 1: PQBP1 transfected lysate(30.5 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: PQBP1 Antibody (1A11) [H00010084-M01] - Detection limit for recombinant GST tagged PQBP1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PQBP1-Antibody-1A11-Sandwich-ELISA-H00010084-M01-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Nicastrin Antibody [NBP2-57365] - Staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57365-img0001.jpg "Immunocytochemistry/Immunofluorescence: Nicastrin Antibody [NBP2-57365] - Staining of human cell line U-2 OS shows localization to cytosol.")
![Western Blot: PQBP1 Antibody [NBP3-38089] - Western blot analysis of various lysates using PQBP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38089_rabbit-pqbp1-pab-912202415143642.jpg "Western Blot: PQBP1 Antibody [NBP3-38089] - Western blot analysis of various lysates using PQBP1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.")
![Western Blot: Nicastrin Antibody (019) [NBP2-89828] - Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell LysateLane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:78 kDa Observed band size:170 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Nicastrin-Antibody-019-Western-Blot-NBP2-89828-img0001.jpg "Western Blot: Nicastrin Antibody (019) [NBP2-89828] - Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell LysateLane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:78 kDa Observed band size:170 kDa (We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Nicastrin Antibody (019) [NBP2-89828] - Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-NCSTN rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NCSTN rabbit monoclonal antibody,at 1:150 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 78 kDa Observed band size: 130 kDa](http://images.novusbio.com/fullsize/Nicastrin-Antibody-019-Immunoprecipitation-NBP2-89828-img0002.jpg)