| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89196-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26333-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24781SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89802-25ul | |||
![Western Blot: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Coronin-1a (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Coronin-1a.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Western-Blot-NBP2-71680-img0002.jpg "Western Blot: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Coronin-1a (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Coronin-1a.")
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Coronin-1a.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71680-img0006.jpg)
![Flow Cytometry: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of Jurkat cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71680-img0005.jpg)
![Western Blot: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Coronin-1a monoclonal antibody.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Western-Blot-NBP2-71680-img0001.jpg)
![Flow Cytometry: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Coronin-1a antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71680-img0004.jpg)
![CyTOF-ready: Coronin-1a Antibody (OTI1A5) - Azide and BSA Free [NBP2-71680] - Analysis of Hela cells, using anti-Coronin-1a antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-OTI1A5-Azide-and-BSA-Free-CyTOF-ready-NBP2-71680-img0003.jpg)
![Western Blot: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIP.](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Western-Blot-NBP2-74848-img0001.jpg "Western Blot: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIP.")
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0007.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0002.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0003.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0004.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0005.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0006.jpg)
![Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/VIP-Antibody-OTI1E8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74848-img0008.jpg)
![Western Blot: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of MTCO2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysateLane 2: THP-1 cell lysate Lane 3: Hela cell lysate Lane 4: human lung tissue lysate Lane 5: human placenta tissue lysate](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Western-Blot-NBP2-67617-img0006.jpg "Western Blot: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of MTCO2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysateLane 2: THP-1 cell lysate Lane 3: Hela cell lysate Lane 4: human lung tissue lysate Lane 5: human placenta tissue lysate")
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0004.jpg)
![Immunohistochemistry-Paraffin: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunohistochemistry-Paraffin-NBP2-67617-img0005.jpg)
![Flow Cytometry: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Flow-Cytometry-NBP2-67617-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MT-CO2 Antibody (SC06-88) [NBP2-67617] - Staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-SC06-88-Immunocytochemistry-Immunofluorescence-NBP2-67617-img0003.jpg)
![Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Western-Blot-NBP2-75531-img0006.jpg "Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate")
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0005.jpg)
![Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Flow-Cytometry-NBP2-75531-img0001.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human colon cancer tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0002.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human breast tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0003.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded mouse epididymis tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0004.jpg)
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414262648.jpg "Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32385, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414333665.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202410334092.jpg)
![Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202416372147.jpg)
![Western Blot: Glut1 Antibody (SA0377) [NBP3-32385] - Western blot analysis of Glut1 on different lysates with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/50,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: HT-29 cell lysate (no heat) Lane 3: HepG2 cell lysate (no heat) Lane 4: NIH/3T3 cell lysate (no heat) Lane 5: L-929 cell lysate (no heat) Lane 6: Mouse brain tissue lysate (no heat) Lane 7: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: Lane 1-7 (left): 20 seconds; Lane 1-7 (right): 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-3920248453511.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0002.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human brain with rabbit monoclonal antibody (1:300).")
![Immunohistochemistry-Paraffin: SOX2 Antibody (017) [NBP2-89196] - Staining of human SOX2 in human testis with rabbit monoclonal antibody (1:300).](http://images.novusbio.com/fullsize/SOX2-Antibody-017-Immunohistochemistry-Paraffin-NBP2-89196-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] - Staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-JF10-30-Immunocytochemistry-Immunofluorescence-NBP2-66994-img0002.jpg "Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] - Staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.")
![Flow Cytometry: Complement C3 Antibody (JF10-30) [NBP2-66994] - Analysis of HepG2 cells with C3 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Complement-C3-Antibody-JF10-30-Flow-Cytometry-NBP2-66994-img0001.jpg)
![Flow Cytometry: Complement C3 Antibody (JF10-30) [NBP2-66994] - Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4c for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4 c. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-flow-cytometry-1542024105623..jpg)
![Western Blot: Complement C3 Antibody (JF10-30) [NBP2-66994] -Western blot analysis of C3 on different lysates with Rabbit anti-C3 antibody at 1/1,000 dilution.Lane 1: Human liver tissue lysateLane 2: HepG2 cell lysateLane 3: HeLa cell lysateLane 4: Mouse liver tissue lysateLane 5: Mouse spleen tissue lysateLane 6: Rat liver tissue lysateLane 7: Rat spleen tissue lysateLysates/proteins at 30 µg/Lane.Predicted band size: 187 kDaObserved band size: 108/37 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-western-blot-1542024105739..jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] -Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-C3 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4 c. Goat Anti-Mouse IgG H&L (iFluor™ ) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-immunocytochemistry-immunofluorescence-15420241102..jpg)
![Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-288202414191539.jpg "Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33093) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-29820241433367.jpg)
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33093, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-298202414304144.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-2920241114843.jpg)
![Flow Cytometry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Flow cytometric analysis of Hela cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33093, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-392024884612.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-288202414233110.jpg "Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241435230.jpg)
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33140, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241492693.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29202410484841.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33140, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-392024884679.jpg)
![Western Blot: Syntenin 1 Antibody (19C1) [NBP3-26333] - Positive Western Blot detected in: HT29 whole cell lysate, U87 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HEPG2 whole cell lysate. All lanes: Syntenin 1 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 32 kDa Observed band size: 32 kDa](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-28220241147217.jpg)
![Immunocytochemistry/Immunofluorescence: Syntenin 1 Antibody (19C1) [NBP3-26333] - Staining of Hela with Syntenin 1 Antibody (19C1) at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 521-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-28220241249364.jpg)
![Flow Cytometry: Syntenin 1 Antibody (19C1) [NBP3-26333] - Overlay Peak curve showing HepG2 cells stained with Syntenin 1 Antibody (19C1) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26333_rabbit-syntenin-1-mab-19c1-282202414312716.jpg)
![Western Blot: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - Western blot analysis of PGP9.5 / UCHL-1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using PGP9.5 / UCHL-1 antibody at 0.2 ug/ml.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NBP2-24781-img0005.jpg "Western Blot: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - Western blot analysis of PGP9.5 / UCHL-1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using PGP9.5 / UCHL-1 antibody at 0.2 ug/ml.")
![Immunohistochemistry-Paraffin: PGP9.5 / UCHL-1 Antibody [NBP2-24781] - IHC analysis of human brain, cortex, using PGP9.5 / UCHL-1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24781-img0003.jpg)
![Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP1-89802] - Immunohistochemical staining of human stomach shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/GAT-1-SLC6A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89802-img0016.jpg "Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP1-89802] - Immunohistochemical staining of human stomach shows no positivity in glandular cells as expected.")
![Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP1-89802] - Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/GAT-1-SLC6A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89802-img0012.jpg)
![Immunohistochemistry-Paraffin: GAT-1/SLC6A1 Antibody [NBP1-89802] - Immunohistochemical staining of human cerebellum shows moderate to strong membranous positivity in neuropil of molecular layer and in Purkinje cell layer.](http://images.novusbio.com/fullsize/GAT-1-SLC6A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89802-img0013.jpg)
