| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03654-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16042-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35827-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-130SS | |||
![Western Blot: Elk-1 Antibody (10U8X1) [NBP3-15631] - Western blot analysis of extracts of various cell lines, using Elk-1 antibody (NBP3-15631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Elk-1-Antibody-10U8X1-Western-Blot-NBP3-15631-img0001.jpg "Western Blot: Elk-1 Antibody (10U8X1) [NBP3-15631] - Western blot analysis of extracts of various cell lines, using Elk-1 antibody (NBP3-15631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunoprecipitation: Elk-1 Antibody (10U8X1) [NBP3-15631] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Elk-1 antibody (NBP3-15631). Western blot was performed from the immunoprecipitate using Elk-1 antibody (NBP3-15631) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Elk-1-Antibody-10U8X1-Immunoprecipitation-NBP3-15631-img0002.jpg)
![Western Blot: HIP1 Related Antibody [NBP3-03654] - Analysis of extracts of various cell lines, using HIP1 Related antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/HIP1-Related-Antibody-Western-Blot-NBP3-03654-img0001.jpg "Western Blot: HIP1 Related Antibody [NBP3-03654] - Analysis of extracts of various cell lines, using HIP1 Related antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Western Blot: beta-Catenin [p Ser552] Antibody [NBP3-05441] - Western blot analysis of extracts of C2C12 cells, using beta-Catenin pAb (NBP3-05441) at 1:1000 dilution or beta-Catenin antibody (A11932).C2C12 cell lysates were treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/beta-Catenin-[p-Ser552]-Antibody-Western-Blot-NBP3-05441-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin [p Ser552] Antibody [NBP3-05441] - Western blot analysis of extracts of C2C12 cells, using beta-Catenin pAb (NBP3-05441) at 1:1000 dilution or beta-Catenin antibody (A11932).C2C12 cell lysates were treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.")
![<b>Biological Strategies Validation. </b>Western Blot: beta-Catenin [p Ser552] Antibody [NBP3-05441] - Western blot analysis of extracts of various cell lines, using beta-Catenin [p Ser552] antibody (NBP3-05441) at 1:1000 dilution. C2C12 cell lysates were treated by CIP at 37C for 1 hour. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight or treated by CIP at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/beta-Catenin-[p-Ser552]-Antibody-Western-Blot-NBP3-05441-img0001.jpg)
![Western Blot: MNX1/HLXB9 Antibody [NBP3-16042] - Western blot analysis of extracts of Rat liver, using MNX1/HLXB9 antibody (NBP3-16042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Western-Blot-NBP3-16042-img0001.jpg "Western Blot: MNX1/HLXB9 Antibody [NBP3-16042] - Western blot analysis of extracts of Rat liver, using MNX1/HLXB9 antibody (NBP3-16042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: MNX1/HLXB9 Antibody [NBP3-16042] - Western blot analysis of extracts of various cell lines, using MNX1/HLXB9 antibody (NBP3-16042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MNX1-HLXB9-Antibody-Western-Blot-NBP3-16042-img0002.jpg)
![Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of lysates from Rat heart, using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-33221_rabbit-uba3-mab-2d2j6-91220241512139.jpg "Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of lysates from Rat heart, using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: UBA3 Antibody (2D2J6) [NBP3-33221] - Western blot analysis of various lysates using UBA3 Rabbit mAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33221_rabbit-uba3-mab-2d2j6-912202414123886.jpg)
![Western Blot: CEND1 Antibody [NBP3-35827] - Western blot analysis of various lysates using CEND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35827_rabbit-cend1-pab-1012202411334026.jpg "Western Blot: CEND1 Antibody [NBP3-35827] - Western blot analysis of various lysates using CEND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Western Blot: CEND1 Antibody [NBP3-35827] - Western blot analysis of various lysates using CEND1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35827_rabbit-cend1-pab-1012202411404065.jpg)
![Western Blot: Tau [p Ser416] Antibody [NB110-93503] - Rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416(control). Imunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/Tau-[p-Ser416]-Antibody-Western-Blot-NB110-93503-img0001.jpg "Western Blot: Tau [p Ser416] Antibody [NB110-93503] - Rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416(control). Imunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).")
![Western Blot: Synaptotagmin 1 [p Thr202] Antibody [NB300-235] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Thr202 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Thr202]-Antibody-Western-Blot-NB300-235-img0001.jpg "Western Blot: Synaptotagmin 1 [p Thr202] Antibody [NB300-235] - Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Thr202 synaptotagmin antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.")
![Immunohistochemistry: Synaptotagmin-1 [p Thr202] Antibody [NB300-235] - Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Thr202 in red and PSD95 in green. Photo courtesy of Gang Liu.](http://images.novusbio.com/fullsize/Synaptotagmin-1-[p-Thr202]-Antibody-Immunohistochemistry-NB300-235-img0002.jpg)
![Western Blot: Dynamin Antibody [NB100-61094] - Western Blot of rat cortex lysate using NB100-61094 at 1:1000.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Western-Blot-NB100-61094-img0002.jpg "Western Blot: Dynamin Antibody [NB100-61094] - Western Blot of rat cortex lysate using NB100-61094 at 1:1000.")
![Western Blot: Dynamin Antibody [NB100-61094] - Rat cortex lysate showing labeling of the ~95k Dynamin protein.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Western-Blot-NB100-61094-img0001.jpg)
![Western Blot: Elk-1 Antibody (3H6D12) [NBP1-47553] - Analysis using ELK1 mouse mAb against truncated ELK1 recombinant protein (1) and K562 cell lysate (2).](http://images.novusbio.com/fullsize/Elk-1-Antibody-3H6D12-Western-Blot-NBP1-47553-img0002.jpg "Western Blot: Elk-1 Antibody (3H6D12) [NBP1-47553] - Analysis using ELK1 mouse mAb against truncated ELK1 recombinant protein (1) and K562 cell lysate (2).")
![Immunohistochemistry-Paraffin: Elk-1 Antibody (3H6D12) [NBP1-47553] - Analysis of human brain tumor tissue, showing nuclear and cytoplasmic localization using ELK1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Elk-1-Antibody-3H6D12-Immunohistochemistry-Paraffin-NBP1-47553-img0003.jpg)
![Western Blot: Presenilin 1 Antibody [NB200-130] - Analysis of Human Presenilin 1 / PSDR1 in androgen-stimulated metastatic adenocarcinoma LNCaP.FGC cells with Presenilin 1 antibody at a dilution of 1:500](http://images.novusbio.com/fullsize/Presenilin-1-Antibody-Western-Blot-NB200-130-img0001.jpg "Western Blot: Presenilin 1 Antibody [NB200-130] - Analysis of Human Presenilin 1 / PSDR1 in androgen-stimulated metastatic adenocarcinoma LNCaP.FGC cells with Presenilin 1 antibody at a dilution of 1:500")
![Western Blot: PER2 Antibody (JE33-79) [NBP3-32735] - Western blot analysis of PER2 on K562 cell lysates with Rabbit anti-PER2 antibody (NBP3-32735) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 137 kDa Observed band size: 180 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32735) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32735_rabbit-per2-mab-je33-79-278202416331229.jpg "Western Blot: PER2 Antibody (JE33-79) [NBP3-32735] - Western blot analysis of PER2 on K562 cell lysates with Rabbit anti-PER2 antibody (NBP3-32735) at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 137 kDa Observed band size: 180 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32735) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PER2 Antibody (JE33-79) [NBP3-32735] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PER2 antibody (NBP3-32735) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32735) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32735_rabbit-per2-mab-je33-79-29820241358556.jpg)